A peptide targeting inflammatory CNS lesions in the EAE rat model of multiple sclerosis
Abstract
Multiple sclerosis is characterized by inflammatory lesions dispersed throughout the central nervous system (CNS) leading to severe neurological handicap. Demyelination, axonal damage, and blood brain barrier alterations are hallmarks of this pathology, whose precise processes are not fully understood. In the experimental autoimmune encephalomyelitis (EAE) rat model that mimics many features of human multiple sclerosis, the phage display strategy was applied to select peptide ligands targeting inflammatory sites in CNS. Due to the large diversity of sequences after phage display selection, a bioinformatics procedure called "PepTeam" designed to identify peptides mimicking naturally occurring proteins was used, with the goal to predict peptides that were not background noise. We identified a circular peptide CLSTASNSC called "Ph48" as an efficient binder of inflammatory regions of EAE CNS sections including small inflammatory lesions of both white and gray matter. Tested on human brain endothelial cells hCMEC/D3, Ph48 was able to bind efficiently when these cells were activated with IL1β to mimic inflammatory conditions. The peptide is therefore a candidate for further analyses of the molecular alterations in inflammatory lesions.
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Chronic neuroinflammation caused by immune disorders triggers the accumulation of misfolded proteins in nervous system cells and peripheral nerves, further leading to neuronal death.
“Chronic neuroinflammation caused by immune disorders triggers the accumulation of misfolded proteins in nervous system cells and peripheral nerves (76), further leading to neuronal death (77).”
From Modulation of the gut–brain axis via the gut microbiota: a new era in treatment of amyotrophic lateral sclerosis (2023) · cites this paper as [77]
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- Authors
- Boiziau, C., Nikolski, M., Mordelet, E., Aussudre, J., Vargas-Sanchez, K., & Petry, K. G.
- Affiliations
- Université de Bordeaux (FR)
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Boiziau, Claudine
- Venue
- Inflammation
- Published
- 2018-03-07
- Volume
- 41
- Issue
- 3
- Pages
- 932-947
- ISSN
- 0360-3997 (ISSN-L)
- 1573-2576
- OpenAlex
- W2789676979
- Cited by
- 10
- References
- 65
- Citations by year
- 2025: 1 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 1
- FWCI
- 0.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Inflammation (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- ANR
- INSERM
- ARSEP
- Conseil regional d'Aquitaine
- Topics
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chemical Synthesis and Analysis
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- multiple sclerosis, experimental autoimmune encephalomyelitis, peptide ligands, phage display, demyelination, blood-brain barrier, brain endothelial cells
- MeSH terms
- Animals, Binding Sites, Cells, Cultured, Central Nervous System, Encephalomyelitis, Autoimmune, Experimental, Humans, Inflammation, Multiple Sclerosis, Peptides, Peptide Library, Endothelial Cells, Rats
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