Effects of different cross-linking conditions on the properties of genipin-cross-linked chitosan/collagen scaffolds for cartilage tissue engineering
Abstract
A cross-linking reagent is required to improve mechanical strength and degradation properties of biopolymers for tissue engineering. To find the optimal preparative method, we prepared diverse genipin-cross-linked chitosan/collagen scaffolds using different genipin concentrations and various cross-linking temperatures and cross-linking times. The compressive strength increased with the increasing of genipin concentration from 0.1 to 1.0%, but when concentration exceeded 1.0%, the compressive strength decreased. Similarly, the compressive strength increased with the increasing of temperature from 4 to 20°C, but when temperature reached 37°C, the compressive strength decreased. Showing a different trend from the above two factors, the effect of cross-linking time on the compressive strength had a single increasing tendency. The other results also demonstrated that the pore size, degradation rate and swelling ratio changed significantly with different cross-linking conditions. Based on our study, 1.0% genipin concentration, 20°C cross-linking temperature and longer cross-linking time are recommended.
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Mineralized collagen is one of the main structural components in natural bone and consists of hydroxyapatite (HA).
“Mineralized collagen is one of the main structural components in the natural bone that consists of hydroxyapatite (HA) [53].”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [53]
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- Authors
- Bi, L., Cao, Z., Hu, Y., Song, Y., Yu, L., Yang, B., Mu, J., Huang, Z., & Han, Y.
- Affiliations
- Xijing Hospital (CN)
- First Affiliated Hospital of Chinese PLA General Hospital (CN)
- Corresponding author
- Han, Yisheng
- Venue
- Journal of Materials Science: Materials in Medicine
- Published
- 2010-11-05
- Volume
- 22
- Issue
- 1
- Pages
- 51-62
- ISSN
- 0957-4530 (ISSN-L)
- 1573-4838
- OpenAlex
- W2073027474
- Cited by
- 215
- References
- 44
- Citations by year
- 2026: 6 · 2025: 15 · 2024: 10 · 2023: 16 · 2022: 18 · 2021: 16 · 2020: 26 · 2019: 22 · 2018: 17 · 2017: 10 · 2016: 10 · 2015: 13 · 2014: 9 · 2013: 9 · 2012: 14
- FWCI
- 3.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Materials Science Materials in Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Science Foundation
- Topics
- Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- genipin, chitosan, collagen, cartilage tissue engineering, compressive strength, crosslinking temperature, crosslinking time, pore size, degradation rate, swelling ratio
- MeSH terms
- Animals, Biocompatible Materials, Biomechanical Phenomena, Cartilage, Cells, Cultured, Collagen, Cross-Linking Reagents, Dose-Response Relationship, Drug, Female, Materials Testing, Rabbits, Temperature, Time Factors, Porosity, Compressive Strength, Tissue Engineering, Iridoids, Chitosan, Tissue Scaffolds, Iridoid Glycosides
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