Cited by 215Collagen: Extraction and CharacterizationHydrogels: synthesis, properties, applicationsBone Tissue Engineering Materials

Effects of different cross-linking conditions on the properties of genipin-cross-linked chitosan/collagen scaffolds for cartilage tissue engineering

Bi, Long, Cao, Zheng, Hu, Yunyu, Song, Yang, Yu, Long, Yang, Bo, Mu, Jihong, Huang, Zhaosong +1 more·2011·Journal of Materials Science: Materials in Medicine

Abstract

A cross-linking reagent is required to improve mechanical strength and degradation properties of biopolymers for tissue engineering. To find the optimal preparative method, we prepared diverse genipin-cross-linked chitosan/collagen scaffolds using different genipin concentrations and various cross-linking temperatures and cross-linking times. The compressive strength increased with the increasing of genipin concentration from 0.1 to 1.0%, but when concentration exceeded 1.0%, the compressive strength decreased. Similarly, the compressive strength increased with the increasing of temperature from 4 to 20°C, but when temperature reached 37°C, the compressive strength decreased. Showing a different trend from the above two factors, the effect of cross-linking time on the compressive strength had a single increasing tendency. The other results also demonstrated that the pore size, degradation rate and swelling ratio changed significantly with different cross-linking conditions. Based on our study, 1.0% genipin concentration, 20°C cross-linking temperature and longer cross-linking time are recommended.

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Crossref · 10.1007/s10856-010-4177-3

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Authors
Bi, L., Cao, Z., Hu, Y., Song, Y., Yu, L., Yang, B., Mu, J., Huang, Z., & Han, Y.
Affiliations
  • Xijing Hospital (CN)
  • First Affiliated Hospital of Chinese PLA General Hospital (CN)
Corresponding author
Han, Yisheng
Venue
Journal of Materials Science: Materials in Medicine
Published
2010-11-05
Volume
22
Issue
1
Pages
51-62
ISSN
  • 0957-4530 (ISSN-L)
  • 1573-4838
OpenAlex
W2073027474
Cited by
215
References
44
Citations by year
2026: 6 · 2025: 15 · 2024: 10 · 2023: 16 · 2022: 18 · 2021: 16 · 2020: 26 · 2019: 22 · 2018: 17 · 2017: 10 · 2016: 10 · 2015: 13 · 2014: 9 · 2013: 9 · 2012: 14
FWCI
3.56
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Materials Science Materials in Medicine (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Science Foundation
Topics
  • Physical Sciences › Materials Science › Biomaterials › Collagen: Extraction and Characterization
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Keywords
genipin, chitosan, collagen, cartilage tissue engineering, compressive strength, crosslinking temperature, crosslinking time, pore size, degradation rate, swelling ratio
MeSH terms
Animals, Biocompatible Materials, Biomechanical Phenomena, Cartilage, Cells, Cultured, Collagen, Cross-Linking Reagents, Dose-Response Relationship, Drug, Female, Materials Testing, Rabbits, Temperature, Time Factors, Porosity, Compressive Strength, Tissue Engineering, Iridoids, Chitosan, Tissue Scaffolds, Iridoid Glycosides

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