Coupling of gelatin to inner surfaces of pore walls in spongy alginate-based scaffolds facilitates the adhesion, growth and differentiation of human bone marrow mesenchymal stromal cells

Petrenko, Yu. A.; Ivanov, R. V.; Petrenko, A. Yu.; Lozinsky, V. I.

doi:10.1007/s10856-011-4323-6

Cited by 78Bone Tissue Engineering MaterialsHydrogels: synthesis, properties, applicationsTissue Engineering and Regenerative Medicine

Coupling of gelatin to inner surfaces of pore walls in spongy alginate-based scaffolds facilitates the adhesion, growth and differentiation of human bone marrow mesenchymal stromal cells

Petrenko, Yu. A., Ivanov, R. V., Petrenko, A. Yu., Lozinsky, V. I.·2011·Journal of Materials Science Materials in Medicine

DOI 10.1007/s10856-011-4323-6

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Abstract

We have developed a novel wide-pore scaffold for cell 3D culturing, based on the technology of freeze-drying of Ca-alginate and gelatin. Two different preparation methodologies were compared: (i) freeze-drying of Na-alginate + gelatin mixed solution followed by the incubation of dried polymer in saturated ethanolic solution of CaCl₂; (ii) freeze-drying of the Na-alginate solution followed by the chemical "activation" of polysaccharide core with divinylsulfone with subsequent gelatin covalent attachment to the inner surfaces of pore walls. The scaffolds produced using the first approach did not provide adhesion and proliferation of human bone marrow mesenchymal stromal cells (MSCs). Conversely, the second approach allowed to obtain scaffolds with a high adherence ability for the cells. When cultured within the latter type of scaffold, MSCs proliferated and were able to differentiate into adipogenic, osteogenic and chondrogenic cell lineages, in response to specific induction stimuli. The results indicate that Ca-alginate wide-pore scaffolds with covalently attached gelatin could be useful for stem cell-based bone, cartilage and adipose tissue engineering.

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Other studies demonstrated that porous scaffolds allowed hBMSCs to proliferate more easily and adhere to scaffold surfaces.
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Cryostructuring of polymer systems. 47. Preparation of wide porous gelatin-based cryostructurates in sterilizing organic media and assessment of the suitability of thus formed matrices as spongy scaffolds for 3D cell culturing
Lozinsky, V. I., Kulakova, V. K., Ivanov, R. V., Petrenko, O., Rogulska, O. Y., & Petrenko, Y. (2017) · e-Polymers · doi:10.1515/epoly-2017-0151 · Cited by 22 · see citing works
“As for the polymeric matrices that are formed as a result of cryogenic procedures employed for the fabrication of such macroporous polymeric scaffolds, there are two their types: cryogels (27–30, 57–59), when the buildup of the 3D polymeric network and its simultaneous crosslinking via chemical or physical bonds occur…”
Background
Alginate Processing Routes to Fabricate Bioinspired Platforms for Tissue Engineering and Drug Delivery
Guarino, V., Altobelli, R., Sala, F. D., Borzacchiello, A., & Ambrosio, L. (2017) · Springer series in biomaterials science and engineering · doi:10.1007/978-981-10-6910-9_4 · Cited by 22 · see citing works
Fabrication and characterization of an antibacterial chitosan-coated allantoin-loaded NaCMC/SA skin scaffold for wound healing applications
Haki, M., Shamloo, A., Eslami, S.-S., Mir-Mohammad-Sadeghi, F., Maleki, S., & Hajizadeh, A. (2023) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2023.127051 · Cited by 20 · see citing works
Preparation of Chitosan Cryostructurates with Controlled Porous Morphology and Their Use as 3D-Scaffolds for the Cultivation of Animal Cells
Sazhnev, N. A., Drozdova, M. G., Rodionov, I. A., Кильдеева, Н. Р., Викторовна, Б. Т., Арнольдовна, М. Е., & Lozinsky, V. I. (2018) · Applied Biochemistry and Microbiology · doi:10.1134/s0003683818050162 · Cited by 20 · see citing works
Influence of succinylation of a wide-pore albumin cryogels on their properties, structure, biodegradability, and release dynamics of dioxidine loaded in such spongy carriers
Lozinsky, V. I., Shchekoltsova, A. O., Sinitskaya, E. S., Vernaya, O. I., Нуждина, А. В., Bakeeva, I. V., Ezernitskaya, M. G., Semenov, A. M., Shabatina, T. I., & Melnikov, M. Y. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.05.251 · Cited by 18 · see citing works
Cryochemical synthesis and antibacterial activity of hybrid nanocomposites based on dioxidine containing Ag and Cu nanoparticles incorporated in biopolymer cryostructurates
Vernaya, O. I., Шабатин, В. П., Нуждина, А. В., Zvukova, N. D., Khvatov, D. I., Semenov, A. M., Лозинский, В. И., Shabatina, T. I., & Melnikov, M. Y. (2017) · Russian Chemical Bulletin · doi:10.1007/s11172-017-1996-0 · Cited by 17 · see citing works
The enhancement of differentiating adipose derived mesenchymal stem cells toward hepatocyte like cells using gelatin cryogel scaffold
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Generation of bone grafts using cryopreserved mesenchymal stromal cells and macroporous collagen‐nanohydroxyapatite cryogels
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Microstructural, mechanical, and histological evaluation of modified alginate-based scaffolds
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“It was shown that this approach is very effective for obtaining spongy alginate cryostructurates [12] that were used as a polymeric base of the wide-pore scaffolds for the three-dimensional cultivation, differentiation, and cryopreservation of stem cells [33,34], as well as were employed as the carriers for the medica…”
Method
Gels as Precursors of Porous Matrices for Use in Foods: a Review
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Biocompatible cryogels: preparation and application
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Induction of Osteogenesis in Rat Bone Tissue Using Cryogenically Structured Porous 3D Materials Containing a Bioregulator
Krasnov, M. S., Шайхалиев, А. И., Korshakov, E. V., Efimenko, M. V., Soloshenkov, P. P., Davidova, T. R., Zvukova, N. D., Sinitskaya, E. S., Yamskova, V. P., Yamskov, I. A., et al. (2019) · Bulletin of Experimental Biology and Medicine · doi:10.1007/s10517-019-04657-z · Cited by 10 · see citing works
“It has also been shown that these materials are effective as macroporous scaffolds for 3D culturing of various cell lines [1,2,10,13].”
Background
Three-dimensional matrixes of natural and synthetic origin for cell biotechnology
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From Biomedical Applications of Alginate towards CVD Implications Linked to COVID-19
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Calotropis gigantea incorporated alginate dialdehyde-gelatin hydrogels for cartilage tissue regeneration in Osteoarthritis
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Alginate-based bionanocomposites in tissue engineering
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Advances for Treatment of Knee OC Defects
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“84 This process is verified in this study for chitosan as the base polymer of the chondral scaffold.”
Background
Biomimetic Gelatin Nanocomposite as a Scaffold for Bone Tissue Repair
Khan, M. N., Hasan, M. M., Islam, M. S., Biswas, S., Rashid, T. U., Mallik, A. K., Zaman, A., Sharmeen, S., Haque, P., & Rahman, M. M. (2017) · Handbook of Composites from Renewable Materials · doi:10.1002/9781119441632.ch166 · Cited by 4 · see citing works
Engineered Fibrous NiTi Scaffolds with Cultured Hepatocytes for Liver Regeneration in Rats
Kokorev, O. V., Marchenko, E. S., Хлусов, И. А., Volinsky, A. A., Yasenchuk, Y. F., & Monogenov, A. N. (2023) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.2c01268 · Cited by 3 · see citing works
TRANSPLANTATION OF CRYOPRESERVED FETAL LIVER CELLS SEEDED INTO MACROPOROUS ALGINATE-GELATIN SCAFFOLDS IN RATS WITH LIVER FAILURE
Grizay, D. V., Lebedinsky, A. S., Ochenashko, O. V., Rogulska, O. Y., Petrenko, Y. А., Lozinsky, V. I., Ivanov, R. V., & Petrenko, O. (2015) · Russian Journal of Transplantology and Artificial Organs · doi:10.15825/1995-1191-2015-3-50-57 · Cited by 3 · see citing works
Changes in Rat Bone Tissue at the Site of the Defect In Vivo under the Effect of a Cryogenically Structured Albumin Sponge Containing a Bioregulator
Krasnov, M. S., Шайхалиев, А. И., Korshakov, E. V., Gasbanov, G. A., Korgoloev, R. S., Sinitskaya, E. S., Sidorsky, E. V., Yamskova, V. P., & Lozinsky, V. I. (2021) · Bulletin of Experimental Biology and Medicine · doi:10.1007/s10517-021-05160-0 · Cited by 3 · see citing works
Production of Synthetic Cryogels and the Study of Porosity Thereof
Savina, I. N., & Galaev, I. Y. (2016) · CRC Press eBooks · doi:10.1201/b19676-5 · Cited by 3 · see citing works
Cryogels and Related Research: A Glance over the Past Few Decades
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Isolation of Human Dental Pulp Stem Cells and Their Characteristics Before and After Cryopreservation
Mazur, S. P., Rogulska, O. Y., Revenko, O. B., Волкова, Н. А., & Petrenko, O. (2021) · Problems of Cryobiology and Cryomedicine · doi:10.15407/cryo31.01.058 · Cited by 2 · see citing works
PHYSICAL MODELING OF BRIQUETTING PROCESSES ON THE BASIS OF THE WASTES OF POLYDISPERSE COKE PARTICLES AND CRYOGELS OF A POLYVINYL ALCOHOL
Anatol’evich, G. P., Reschetilowski, V. P., Манжай, В. Н., Zyatikov, P., & Васильевич, С. В. (2018) · Vestnik Tomskogo gosudarstvennogo universiteta Matematika i mekhanika · doi:10.17223/19988621/53/7 · Cited by 2 · see citing works
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Choice of Vitriﬁcation Mode for Macroporous Matrices Seeded with Mesenchymal Stromal Cells
Trufanova, N. A., Kovalenko, I., & Petrenko, O. (2020) · Problems of Cryobiology and Cryomedicine · doi:10.15407/cryo30.01.077 · Cited by 1 · see citing works
Cryopreservation of mesenchymal stem cells within macroporous matrices after sucrose pretreatment
Trufanova, N. A., Rogulska, O. Y., Semenchenko, O. A., Mishchenko, O., & Petrenko, O. (2025) · Problems of Cryobiology and Cryomedicine · doi:10.15407/cryo35.02.103 · Cited by 1 · see citing works
Modulation of mesenchymal stromal cells properties by the microenvironment in 3D culture
Petrenko, O., Rogulska, O. Y., Trufanova, N. A., Trufanov, O., Hubenia, O., Revenko, O. B., & Cherkashina, D. V. (2023) · ScienceRise Biological Science · doi:10.15587/2519-8025.2023.288082 · Cited by 1 · see citing works
“However, chemically coupled gelatin to the surface of pores imparted adhesiveness, accompanied by cell proliferation [9].”
BackgroundMethodInfluential
Bioengineering of Mesenchymal-Stromal-Cell-Based 3D Constructs with Different Cell Organizations
Trufanova, N. A., Trufanov, O., Божок, Г. А., Oberemok, R., Revenko, O. B., & Petrenko, O. (2025) · doi:10.3390/engproc2024081008 · Cited by 1 · see citing works
“Macroporous sponge-like scaffolds from adhesive materials provide wide opportunities for cell attachment, interactions, migration, and proliferation [9].”
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BackgroundMethodInfluential
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Authors: Petrenko, Y. A., Ivanov, R. V., Petrenko, A. Y., & Lozinsky, V. I.
Affiliations: Russian Academy of Sciences (RU)
ORCID: Lozinsky, V. I.
Venue: Journal of Materials Science Materials in Medicine
Publisher: Springer Science and Business Media LLC
Published: 2011-04-28
Volume: 22
Issue: 6
Pages: 1529-1540
ISSN: 0957-4530 (ISSN-L)
1573-4838
DOI: 10.1007/s10856-011-4323-6
OpenAlex: W1982180375
Cited by: 78
References: 53
Citations by year: 2025: 2 · 2023: 6 · 2022: 6 · 2021: 10 · 2020: 5 · 2019: 2 · 2018: 7 · 2017: 8 · 2016: 6 · 2015: 6 · 2014: 10 · 2013: 4 · 2012: 4
FWCI: 3.79
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Journal of Materials Science Materials in Medicine (publishedVersion)
PubMed (publishedVersion)
Topics: Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords: freeze-drying, cell adhesion, cell proliferation, osteogenic differentiation, adipogenesis, chondrogenic differentiation, tissue engineering, bone tissue engineering, cartilage tissue engineering, adipose tissue engineering
MeSH terms: Adult, Alginates, Bone Marrow Cells, Cell Adhesion, Cell Differentiation, Cells, Cultured, Elasticity, Gelatin, Hexuronic Acids, Humans, Surface Properties, Porosity, Stromal Cells, Cell Culture Techniques, Coated Materials, Biocompatible, Glucuronic Acid, Cell Proliferation, Tissue Scaffolds, Mesenchymal Stem Cells

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Coupling of gelatin to inner surfaces of pore walls in spongy alginate-based scaffolds facilitates the adhesion, growth and differentiation of human bone marrow mesenchymal stromal cells

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