Cited by 484Bone Tissue Engineering MaterialsHydrogels: synthesis, properties, applicationsTissue Engineering and Regenerative Medicine

Preparation of porous scaffolds by using freeze-extraction and freeze-gelation methods

Ho, Ming-Hua, Kuo, Pei-Yun, Hsieh, Hsyue-Jen, Hsien, Tzu-Yang, Hou, Lein-Tuan, Lai, Juin-Yih, Wang, Da-Ming·2004·Biomaterials

Abstract

Freeze-fixation and freeze-gelation methods are presented in this paper which can be used to prepare highly porous scaffolds without using the time and energy consuming freeze-drying process. The porous structure was generated during the freeze of a polymer solution, following which either the solvent was extracted by a non-solvent or the polymer was gelled under the freezing condition; thus, the porous structure would not be destructed during the subsequent drying stage. Compared with the freeze-drying method, the presented methods are time and energy-saving, with less residual solvent, and easier to be scaled up. Besides, the problem of formation of surface skin can be resolved and the limitation of using solvent with low boiling point can be lifted by the presented methods. With the freeze-extraction and freeze-gelation methods, porous PLLA, PLGA, chitosan and alginate scaffolds were successfully fabricated. In addition to the presentation of the morphologies of the fabricated scaffolds, preliminary data of cell culture on them are as well included in the present work.

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Crossref · 10.1016/s0142-9612(03)00483-6

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26 of 26 references verified0 citation errors · 0 not checkable

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Authors
Ho, M.-H., Kuo, P.-Y., Hsieh, H.-J., Hsien, T.-Y., Hou, L.-T., Lai, J.-Y., & Wang, D.-M.
Affiliations
  • National Taiwan University (TW)
  • Environmental Protection Administration (TW)
Venue
Biomaterials
Publisher
Elsevier BV
Published
2004-01
Volume
25
Issue
1
Pages
129-138
ISSN
0142-9612 (ISSN-L)
OpenAlex
W2057723544
Cited by
484
References
26
Citations by year
2026: 6 · 2025: 8 · 2024: 15 · 2023: 14 · 2022: 16 · 2021: 19 · 2020: 20 · 2019: 30 · 2018: 33 · 2017: 34 · 2016: 34 · 2015: 31 · 2014: 33 · 2013: 23 · 2012: 25
FWCI
5.77
Type
journal article
Language
en
Open access
closed
Locations
  • Biomaterials (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Science Council
  • National Taiwan University
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
Keywords
porous scaffolds, freeze extraction, freeze-gelation, freeze-drying alternative, polymer gelation, poly(L-lactic acid), PLGA, chitosan, alginate, scaffold fabrication
MeSH terms
Polylactic Acid-Polyglycolic Acid Copolymer, Alginates, Animals, Biocompatible Materials, Cell Adhesion, Cell Division, Cell Line, Chitin, Crystallization, Freezing, Gels, Hexuronic Acids, Osteoblasts, Polyesters, Polyglycolic Acid, Polymers, Surface Properties, Porosity, Cell Culture Techniques, Lactic Acid, Glucuronic Acid, Tissue Engineering, Chitosan, Rats

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