The TOM complex is involved in the release of superoxide anion from mitochondria
Abstract
Available data indicate that superoxide anion (O(2)(*-) ) is released from mitochondria, but apart from VDAC (voltage dependent anion channel), the proteins involved in its transport across the mitochondrial outer membrane still remain elusive. Using mitochondria of the yeast Saccharomyces cerevisiae mutant depleted of VDAC (Deltapor1 mutant) and the isogenic wild type, we studied the role of the TOM complex (translocase of the outer membrane) in the efflux of O(2)(*-) from the mitochondria. We found that blocking the TOM complex with the fusion protein pb(2)-DHFR decreased O(2)(*-) release, particularly in the case of Deltapor1 mitochondria. We also observed that the effect of the TOM complex blockage on O(2)(*-) release from mitochondria coincided with the levels of O(2)(*-) release as well as with levels of Tom40 expression in the mitochondria. Thus, we conclude that the TOM complex participates in O(2)(*-) release from mitochondria.
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More recent data from mutant yeast strains lacking VDAC indicate that the translocase of the outer membrane complex participates in superoxide release from mitochondria in the absence of VDAC.
“More recent data from mutant yeast strains lacking VDAC indicate that the translocase of the outer membrane complex participates in superoxide release from mitochondria in the absence of VDAC (55).”
From Redox regulation of mitochondrial function (2012) · cites this paper as [55]
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- Authors
- Budzińska, M., Gałgańska, H., Karachitos, A., Wojtkowska, M., & Kmita, H.
- Affiliations
- Adam Mickiewicz University in Poznań (PL)
- Corresponding author
- Kmita, Hanna
- Venue
- Journal of Bioenergetics and Biomembranes
- Published
- 2009-08
- Volume
- 41
- Issue
- 4
- Pages
- 361-367
- ISSN
- 0145-479X (ISSN-L)
- 1573-6881
- OpenAlex
- W2075102823
- Cited by
- 31
- References
- 44
- Citations by year
- 2024: 3 · 2022: 1 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 1.47
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Bioenergetics and Biomembranes (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Forskningsrådet om Hälsa, Arbetsliv och Välfärd
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › ATP Synthase and ATPases Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- TOM complex, superoxide, mitochondrial outer membrane, voltage-dependent anion channel, Tom40, Saccharomyces cerevisiae, mitochondrial respiration
- MeSH terms
- Mitochondrial Precursor Protein Import Complex Proteins, Carrier Proteins, Saccharomyces cerevisiae, Superoxides, Ion Channel Gating, Saccharomyces cerevisiae Proteins, Mitochondrial Membranes
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