SIRT1 activation attenuates palmitate induced apoptosis in C2C12 muscle cells
Abstract
Background Type 2 diabetes is characterized by insulin resistance, which manifests mainly in skeletal muscles. SIRT1 has been found to play a role in the insulin signaling pathway. However, the molecular underpinnings of SIRT1's function in palmitate fatty acid-induced apoptosis still need to be better understood. Methods In this research, skeletal muscle cells are treated with palmitate to be insulin resistant. It is approached that SIRT1 is downregulated in C2C12 muscle cells during palmitate-induced apoptosis and that activating SIRT1 mitigates this effect. Results Based on these findings, palmitate-induced apoptosis suppressed mitochondrial biogenesis by lowering PGC-1 expression, while SIRT1 overexpression boosted. The SIRT1 inhibitor sirtinol, on the other hand, decreased mitochondrial biogenesis under the same conditions. This research also shows that ROS levels rise in the conditions necessary for apoptosis induction by palmitate, and ROS inhibitors can mitigate this effect. This work demonstrated that lowering ROS levels by boosting SIRT1 expression inhibited apoptotic induction in skeletal muscle cells. Conclusion This study's findings suggested that SIRT1 can improve insulin resistance in type 2 diabetes by slowing the rate of lipo-apoptosis and boosting mitochondrial biogenesis, among other benefits.
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Myeloma cells have the capacity to reprogram normal bone marrow adipocytes, giving them the capacity to resorb bone in myeloma patients who are in remission.
“According to Liu et al.‘s study, myeloma cells have the capacity to reprogram normal BM adipocytes, giving them the capacity to resorb bone in myeloma patients who are in remission [282, 283].”
From Mesenchymal stromal cells in bone marrow niche of patients with multiple myeloma: a double-edged sword (2025) · cites this paper as [283]
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- Authors
- Taheripak, G., Sabeti, N., Najar, N., Razavi, Z., Saharkhiz, S., & Alipourfard, I.
- Affiliations
- Iran University of Medical Sciences (IR)
- Islamic Azad University, Tehran (IR)
- University of Ottawa (CA)
- Institute of Physical Chemistry (PL)
- Corresponding author
- Alipourfard, Iraj
- Venue
- Molecular Biology Reports
- Published
- 2024-02-24
- Volume
- 51
- Issue
- 1
- Pages
- 354-354
- ISSN
- 0301-4851 (ISSN-L)
- 1573-4978
- OpenAlex
- W4392131481
- Cited by
- 33
- References
- 44
- Citations by year
- 2026: 5 · 2025: 16 · 2024: 12
- FWCI
- 9.18
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Molecular Biology Reports (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- SIRT1, palmitate, apoptosis, C2C12 muscle cells, skeletal muscle, insulin resistance, type 2 diabetes, mitochondrial biogenesis, PGC-1, reactive oxygen species, sirtinol
- MeSH terms
- Cell Line, Diabetes Mellitus, Type 2, Humans, Insulin, Insulin Resistance, Palmitates, Apoptosis, Reactive Oxygen Species, Muscle, Skeletal, Muscle Fibers, Skeletal, Sirtuin 1
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