Cited by 33Sirtuins and Resveratrol in MedicineAdipose Tissue and MetabolismMetabolism, Diabetes, and Cancer

SIRT1 activation attenuates palmitate induced apoptosis in C2C12 muscle cells

Taheripak, Gholamreza, Sabeti, Niusha, Najar, Naba, Razavi, Zahrasadat, Saharkhiz, Saber, Alipourfard, Iraj·2024·Molecular Biology Reports

Abstract

Background Type 2 diabetes is characterized by insulin resistance, which manifests mainly in skeletal muscles. SIRT1 has been found to play a role in the insulin signaling pathway. However, the molecular underpinnings of SIRT1's function in palmitate fatty acid-induced apoptosis still need to be better understood. Methods In this research, skeletal muscle cells are treated with palmitate to be insulin resistant. It is approached that SIRT1 is downregulated in C2C12 muscle cells during palmitate-induced apoptosis and that activating SIRT1 mitigates this effect. Results Based on these findings, palmitate-induced apoptosis suppressed mitochondrial biogenesis by lowering PGC-1 expression, while SIRT1 overexpression boosted. The SIRT1 inhibitor sirtinol, on the other hand, decreased mitochondrial biogenesis under the same conditions. This research also shows that ROS levels rise in the conditions necessary for apoptosis induction by palmitate, and ROS inhibitors can mitigate this effect. This work demonstrated that lowering ROS levels by boosting SIRT1 expression inhibited apoptotic induction in skeletal muscle cells. Conclusion This study's findings suggested that SIRT1 can improve insulin resistance in type 2 diabetes by slowing the rate of lipo-apoptosis and boosting mitochondrial biogenesis, among other benefits.

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Crossref · 10.1007/s11033-024-09250-w

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  • Myeloma cells have the capacity to reprogram normal bone marrow adipocytes, giving them the capacity to resorb bone in myeloma patients who are in remission.

    “According to Liu et al.‘s study, myeloma cells have the capacity to reprogram normal BM adipocytes, giving them the capacity to resorb bone in myeloma patients who are in remission [282, 283].”

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Authors
Taheripak, G., Sabeti, N., Najar, N., Razavi, Z., Saharkhiz, S., & Alipourfard, I.
Affiliations
  • Iran University of Medical Sciences (IR)
  • Islamic Azad University, Tehran (IR)
  • University of Ottawa (CA)
  • Institute of Physical Chemistry (PL)
Corresponding author
Alipourfard, Iraj
Venue
Molecular Biology Reports
Published
2024-02-24
Volume
51
Issue
1
Pages
354-354
ISSN
  • 0301-4851 (ISSN-L)
  • 1573-4978
OpenAlex
W4392131481
Cited by
33
References
44
Citations by year
2026: 5 · 2025: 16 · 2024: 12
FWCI
9.18
Type
journal article
Language
en
Open access
closed
Locations
  • Molecular Biology Reports (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Geriatrics and Gerontology › Sirtuins and Resveratrol in Medicine
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords
SIRT1, palmitate, apoptosis, C2C12 muscle cells, skeletal muscle, insulin resistance, type 2 diabetes, mitochondrial biogenesis, PGC-1, reactive oxygen species, sirtinol
MeSH terms
Cell Line, Diabetes Mellitus, Type 2, Humans, Insulin, Insulin Resistance, Palmitates, Apoptosis, Reactive Oxygen Species, Muscle, Skeletal, Muscle Fibers, Skeletal, Sirtuin 1

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