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The effect of thermotherapy using magnetic nanoparticles on rat malignant glioma
Abstract
Thermotherapy using magnetic nanoparticles is a new technique for interstitial hyperthermia and thermoablation based on magnetic field-induced excitation of biocompatible superparamagnetic nanoparticles. To evaluate the potential of this technique for minimally invasive treatment, we carried out a systematic analysis of its effects on experimental glioblastoma multiforme in a rat tumor model. Tumors were induced by implantation of RG-2-cells into the brains of 120 male Fisher rats. Animals were randomly allocated to 10 groups of 12 rats each, including controls. Animals received two thermotherapy treatments following a single intratumoral injection of two different magnetic fluids (dextran- or aminosilane-coated iron-oxide nanoparticles). Treatment was carried out on days four and six after tumor induction using an alternating magnetic field applicator system operating at a frequency of 100 kHz and variable field strength of 0-18 kA/m. The effectiveness of treatment was determined by the survival time of the animals and histopathological examinations of the brain and the tumor.Thermotherapy with aminosilane-coated nanoparticles led up to 4.5-fold prolongation of survival over controls, while the dextran-coated particles did not indicate any advantage. Intratumoral deposition of the aminosilane-coated particles was found to be stable, allowing for serial thermotherapy treatments without repeated injection. Histological and immunohistochemical examinations after treatment revealed large necrotic areas close to particle deposits, a decreased proliferation rate and a reactive astrogliosis adjacent to the tumor.Thus, localized interstitial thermotherapy with magnetic nanoparticles has an antitumoral effect on malignant brain tumors. This method is suitable for clinical use and may be a novel strategy for treating malignant glioma, which cannot be treated successfully today. The optimal treatment schedules and potential combinations with other therapies need to be defined in further studies.
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“In addition, MRI for evaluation of tumor progression cannot be properly performed after MFH, because of the artifacts caused by the very high amounts of SPION that accumulate in the lesion [219].”
From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [219]
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Metadata
- Authors
- Jordan, A., Scholz, R., Maier-Hauff, K., van Landeghem, F. K. H., Waldoefner, N., Teichgraeber, U., Pinkernelle, J., Bruhn, H., Neumann, F., Thiesen, B., von Deimling, A., & Felix, R.
- Affiliations
- Charité – Universitätsmedizin Berlin (DE)
- Corresponding author
- Jordan, Andreas
- ORCID
- Bruhn, Harald
- Venue
- Journal of Neuro-Oncology
- Published
- 2005-11-29
- Volume
- 78
- Issue
- 1
- Pages
- 7-14
- ISSN
- 0167-594X (ISSN-L)
- 1573-7373
- OpenAlex
- W2093807603
- Cited by
- 459
- References
- 23
- Citations by year
- 2026: 5 · 2025: 10 · 2024: 11 · 2023: 17 · 2022: 19 · 2021: 30 · 2020: 23 · 2019: 20 · 2018: 31 · 2017: 30 · 2016: 20 · 2015: 28 · 2014: 32 · 2013: 29 · 2012: 28
- FWCI
- 10.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Neuro-Oncology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Regional Development Fund
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Physical Sciences › Engineering › Biomedical Engineering › Ultrasound and Hyperthermia Applications
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- magnetic nanoparticles, heat therapy, glioblastoma, high-grade glioma, interstitial hyperthermia, thermal ablation, superparamagnetic iron oxide nanoparticles, dextran-coated nanoparticles, alternating magnetic field, intratumoral injection, tumor necrosis, astrocyte activation, rat tumor model, survival
- MeSH terms
- Animals, Brain Neoplasms, Glioma, Hyperthermia, Induced, Immunohistochemistry, Magnetic Resonance Imaging, Magnetics, Male, Rats, Inbred F344, Survival Analysis, Nanostructures, Rats
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