Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Striatum After Various Durations of Transient Cerebral Ischemia in Gerbils
Abstract
Ischemic damage occurs well in vulnerable regions of the brain, including the hippocampus and striatum. In the present study, we examined neuronal damage/death and glial changes in the striatum 4 days after 5, 10, 15 and 20 min of transient cerebral ischemia using the gerbil. Spontaneous motor activity was increased with the duration time of ischemia-reperfusion (I-R). To examine neuronal damage, we used Fluoro-Jade B (F-J B, a marker for neuronal degeneration) histofluorescence staining. F-J B positive cells were detected only in the 20 min ischemia-group, not in the other groups. In addition, we examined gliosis of astrocytes and microglia using anti-glial fibrillary acidic protein (GFAP) and anti- ionized calcium-binding adapter molecule 1 (Iba-1), respectively. In the 5 min ischemia-group, GFAP-immunoreactive astrocytes were distinctively increased in number, and the immunoreactivity was stronger than that in the sham-group. In the 10, 15 and 20 min ischemia-groups, GFAP-immunoreactivity was more increased with the duration of I-R. On the other hand, the immunoreactivity and the number of Iba-1-immunoreactive microglia were distinctively increased in the 5 and 10 min ischemia-groups. In the 15 min ischemia-group, cell bodies of microglia were largest, and the immunoreactivity was highest; however, in the 20 min ischemia-group, the immunoreactivity was low compared to the 15 min ischemia-group. The results of western blotting for GFAP and Iba-1 were similar to the immunohistochemical data. In brief, these findings showed that neuronal death could be detected only in the 20 min ischemia-group 4 days after I-R, and the change pattern of astrocytes and microglia were apparently different according to the duration time of I-R.
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Fluoro-Jade B specifically stains degenerating neurons by binding to a currently unknown, most likely poly-amine, basic substance in the neuron.
“Fluoro-Jade B as anionic dye specifically stains degenerating neurons by binding to a currently unknown, most likely a poly-amine, basic substance in the neuron.”
From Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress (2014) · cites this paper as [59]
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Retracted
- Authors
- Ohk, T. G., Yoo, K.-Y., Park, S. M., Shin, B. N., Kim, I. H., Park, J. H., Ahn, H. C., Lee, Y. J., Kim, M. J., Kim, T. Y., Won, M.-H., & Cho, J. H.
- Affiliations
- Hallym University Sacred Heart Hospital (KR)
- Gangneung–Wonju National University (KR)
- Kangwon National University (KR)
- Rural Development Administration (KR)
- Corresponding author
- Won, Moo-Ho
- Cho, Jun Hwi
- ORCID
- Venue
- Neurochemical Research
- Published
- 2012-01-05
- Volume
- 37
- Issue
- 4
- Pages
- 826-834
- ISSN
- 0364-3190 (ISSN-L)
- 1573-6903
- OpenAlex
- W1989746202
- Cited by
- 29
- References
- 52
- Citations by year
- 2024: 2 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 4 · 2014: 2 · 2013: 5 · 2012: 2
- FWCI
- 2.08
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Neurochemical Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Health Sciences › Medicine › Epidemiology › Acute Ischemic Stroke Management
- Keywords
- transient cerebral ischemia, ischemia-reperfusion, striatum, neuronal injury, neuronal cell death, gliosis, astrocytes, microglia, glial fibrillary acidic protein, Iba1, Fluoro-Jade B, gerbils, ischemic time, spontaneous motor activity
- MeSH terms
- Animals, Ischemic Attack, Transient, Corpus Striatum, Fluoresceins, Fluorescent Dyes, Gerbillinae, Gliosis, Male, Motor Activity, Neurons, Organic Chemicals, Time Factors
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