Cited by 29Neuroinflammation and Neurodegeneration MechanismsNeurological Disease Mechanisms and TreatmentsAcute Ischemic Stroke Management

Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Striatum After Various Durations of Transient Cerebral Ischemia in Gerbils

Ohk, Taek Geun, Yoo, Ki-Yeon, Park, Seung Min, Shin, Bich Na, Kim, In Hye, Park, Joon Ha, Ahn, Hee Cheol, Lee, Young Joo +4 more·2012·Neurochemical Research

Abstract

Ischemic damage occurs well in vulnerable regions of the brain, including the hippocampus and striatum. In the present study, we examined neuronal damage/death and glial changes in the striatum 4 days after 5, 10, 15 and 20 min of transient cerebral ischemia using the gerbil. Spontaneous motor activity was increased with the duration time of ischemia-reperfusion (I-R). To examine neuronal damage, we used Fluoro-Jade B (F-J B, a marker for neuronal degeneration) histofluorescence staining. F-J B positive cells were detected only in the 20 min ischemia-group, not in the other groups. In addition, we examined gliosis of astrocytes and microglia using anti-glial fibrillary acidic protein (GFAP) and anti- ionized calcium-binding adapter molecule 1 (Iba-1), respectively. In the 5 min ischemia-group, GFAP-immunoreactive astrocytes were distinctively increased in number, and the immunoreactivity was stronger than that in the sham-group. In the 10, 15 and 20 min ischemia-groups, GFAP-immunoreactivity was more increased with the duration of I-R. On the other hand, the immunoreactivity and the number of Iba-1-immunoreactive microglia were distinctively increased in the 5 and 10 min ischemia-groups. In the 15 min ischemia-group, cell bodies of microglia were largest, and the immunoreactivity was highest; however, in the 20 min ischemia-group, the immunoreactivity was low compared to the 15 min ischemia-group. The results of western blotting for GFAP and Iba-1 were similar to the immunohistochemical data. In brief, these findings showed that neuronal death could be detected only in the 20 min ischemia-group 4 days after I-R, and the change pattern of astrocytes and microglia were apparently different according to the duration time of I-R.

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Crossref · 10.1007/s11064-011-0678-9

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Authors
Ohk, T. G., Yoo, K.-Y., Park, S. M., Shin, B. N., Kim, I. H., Park, J. H., Ahn, H. C., Lee, Y. J., Kim, M. J., Kim, T. Y., Won, M.-H., & Cho, J. H.
Affiliations
  • Hallym University Sacred Heart Hospital (KR)
  • Gangneung–Wonju National University (KR)
  • Kangwon National University (KR)
  • Rural Development Administration (KR)
Corresponding author
  • Won, Moo-Ho
  • Cho, Jun Hwi
Venue
Neurochemical Research
Published
2012-01-05
Volume
37
Issue
4
Pages
826-834
ISSN
  • 0364-3190 (ISSN-L)
  • 1573-6903
OpenAlex
W1989746202
Cited by
29
References
52
Citations by year
2024: 2 · 2021: 3 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 4 · 2014: 2 · 2013: 5 · 2012: 2
FWCI
2.08
Type
journal article
Language
en
Open access
closed
Locations
  • Neurochemical Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
  • Health Sciences › Medicine › Epidemiology › Acute Ischemic Stroke Management
Keywords
transient cerebral ischemia, ischemia-reperfusion, striatum, neuronal injury, neuronal cell death, gliosis, astrocytes, microglia, glial fibrillary acidic protein, Iba1, Fluoro-Jade B, gerbils, ischemic time, spontaneous motor activity
MeSH terms
Animals, Ischemic Attack, Transient, Corpus Striatum, Fluoresceins, Fluorescent Dyes, Gerbillinae, Gliosis, Male, Motor Activity, Neurons, Organic Chemicals, Time Factors

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