Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration
Open accessAbstract
Fluoro-Jade B, like its predecessor Fluoro-Jade, is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. However, Fluoro-Jade B has an even greater specific affinity for degenerating neurons. This notion is supported by the conspicuous staining of degenerating neuronal elements with minimal background staining. This improved signal-to-noise ratio means that fine neuronal processes including distal dendrites, axons and axon terminals can be more readily detected and documented. Although the staining time and dye concentration are reduced, the method is as rapid, simple and reliable as the original Fluoro-Jade technique. Like Fluoro-Jade, Fluoro-Jade B is compatible with a number of other labeling procedures including immunofluorescent and fluorescent Nissl techniques.
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Fluoro-Jade B specifically stains degenerating neurons by binding to a currently unknown, most likely poly-amine, basic substance in the neuron.
“Fluoro-Jade B as anionic dye specifically stains degenerating neurons by binding to a currently unknown, most likely a poly-amine, basic substance in the neuron.”
From Aggrecan, link protein and tenascin-R are essential components of the perineuronal net to protect neurons against iron-induced oxidative stress (2014) · cites this paper as [60]
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Schmued, L. C., Albertson, C., & Slikker, W. (1997) · Brain Research · doi:10.1016/s0006-8993(96)01387-x · Cited by 995 · see citing works
Self-citationSilver Impregnation of Degenerating Axons in the Central Nervous System: A Modified Technic
Nauta, W. J. H., & Gygax, P. A. (1954) · Stain Technology · doi:10.3109/10520295409115448 · Cited by 836 · see citing works
Silver Impregnation of Degenerating Axons in the Central Nervous System: A Modified Technic
Nauta, W. J. H., & Gygax, P. A. (1993) · Birkhäuser Boston eBooks · doi:10.1007/978-1-4684-7920-1_3 · Cited by 730 · see citing works
Kainic acid: a powerful neurotoxic analogue of glutamate
Olney, J. W., Rhee, V., & Ho, O. L. (1974) · Brain Research · doi:10.1016/0006-8993(74)90640-4 · Cited by 706 · see citing works
de Olmos, J. S., Beltramino, C. A., & Lorenzo, S. D. O. D. (1994) · Neurotoxicology and Teratology · doi:10.1016/0892-0362(94)90033-7 · Cited by 237 · see citing works
Systemic administration of MPTP induces thalamic neuronal degeneration in mice
Freyaldenhoven, T. E., Ali, S. F., & Schmued, L. C. (1997) · Brain Research · doi:10.1016/s0006-8993(97)00045-0 · Cited by 84 · see citing works
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Glucagon-like peptide-1 receptor is involved in learning and neuroprotection
During, M. J., Cao, L., Zuzga, D. S., Francis, J. S., Fitzsimons, H. L., Jiao, X., Bland, R. J., Klugmann, M., Banks, W. A., Drucker, D. J., et al. (2003) · Nature Medicine · doi:10.1038/nm919 · Cited by 851 · see citing works
Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice
Lasagna-Reeves, C. A., Castillo-Carranza, D. L., Sengupta, U., Clos, A. L., Jackson, G. R., & Kayed, R. (2011) · Molecular Neurodegeneration · doi:10.1186/1750-1326-6-39 · Cited by 633 · see citing works
Tiwari, S. K., Agarwal, S., Seth, B., Yadav, A., Nair, S., Bhatnagar, P., Karmakar, M., Kumari, M., Chauhan, L. K. S., Patel, D. K., et al. (2014) · ACS Nano · doi:10.1021/nn405077y · Cited by 558 · see citing works
Suh, S. W., Gum, E. T., Hamby, A. M., Chan, P. H., & Swanson, R. A. (2007) · Journal of Clinical Investigation · doi:10.1172/jci30077 · Cited by 419 · see citing works
Synchronous neuronal activity is a signal for axonal sprouting after cortical lesions in the adult
Carmichael, S. T., & Chesselet, M.-F. (2002) · The Journal of Neuroscience · doi:10.1523/jneurosci.22-14-06062.2002 · Cited by 371 · see citing works
Neurogenesis in adolescent brain is potently inhibited by ethanol
Crews, F. T., Mdzinarishvili, A., Kim, D., He, J., & Nixon, K. (2006) · Neuroscience · doi:10.1016/j.neuroscience.2005.08.090 · Cited by 265 · see citing works
Lodato, S., Rouaux, C., Quast, K. B., Jantrachotechatchawan, C., Studer, M., Hensch, T. K., & Arlotta, P. (2011) · Neuron · doi:10.1016/j.neuron.2011.01.015 · Cited by 244 · see citing works
Barrett, R. M., Malvaez, M., Kramar, E., Matheos, D. P., Arrizon, A., Cabrera, S. M., Lynch, G., Greene, R. W., & Wood, M. A. (2011) · Neuropsychopharmacology · doi:10.1038/npp.2011.61 · Cited by 243 · see citing works
Dinocourt, C., Petanjek, Z., Freund, T. F., Ben‐Ari, Y., & Esclapez, M. (2003) · Journal of Comparative Neurology · doi:10.1002/cne.10622 · Cited by 208 · see citing works
Melchor, J. P., Pawlak, R., & Strickland, S. (2003) · The Journal of Neuroscience · doi:10.1523/jneurosci.23-26-08867.2003 · Cited by 202 · see citing works
Indole‐3‐propionic acid attenuates neuronal damage and oxidative stress in the ischemic hippocampus
Hwang, I. K., Yoo, K., Li, H., Park, O. K., Lee, C. H., Choi, J. H., Jeong, Y., Lee, Y. L., Kim, Y., Kwon, Y., et al. (2009) · Journal of Neuroscience Research · doi:10.1002/jnr.22030 · Cited by 186 · see citing works
Suttkus, A., Rohn, S., Weigel, S., Glöckner, P., Arendt, T., & Morawski, M. (2014) · Cell Death & Disease · doi:10.1038/cddis.2014.25 · Cited by 168 · see citing works
Kainate Seizures Cause Acute Dendritic Injury and Actin Depolymerization<i>In Vivo</i>
Zeng, L.-H., Xu, L., Rensing, N. R., Sinatra, P. M., Rothman, S. M., & Wong, M. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.0983-07.2007 · Cited by 158 · see citing works
Pfarr, S., Meinhardt, M. W., Klee, M. L., Hansson, A. C., Vengeliene, V., Schonig, K., Bartsch, D., Hope, B. T., Spanagel, R., & Sommer, W. H. (2015) · Journal of Neuroscience · doi:10.1523/jneurosci.0684-15.2015 · Cited by 143 · see citing works
Volosin, M., Trotter, C., Cragnolini, A., Kenchappa, R. S., Light, M., Hempstead, B. L., Carter, B. D., & Friedman, W. J. (2008) · The Journal of Neuroscience · doi:10.1523/jneurosci.2841-08.2008 · Cited by 149 · see citing works
A molecular pathway of neurodegeneration linking α-synuclein to ApoE and Aβ peptides
Gallardo, G., Schlüter, O. M., & Südhof, T. C. (2008) · Nature Neuroscience · doi:10.1038/nn2058 · Cited by 145 · see citing works
Potter, K. A., Buck, A. C., Self, W. K., Callanan, M. E., Sunil, S., & Capadona, J. R. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.05.035 · Cited by 129 · see citing works
Puente, E. C., Silverstein, J., Bree, A. J., Musikantow, D. R., Wozniak, D. F., Maloney, S., Daphna-Iken, D., & Fisher, S. J. (2010) · Diabetes · doi:10.2337/db09-1495 · Cited by 103 · see citing works
Obesity exacerbates chemically induced neurodegeneration
Sriram, K., Benkovic, S. A., Miller, D. B., & O’Callaghan, J. P. (2002) · Neuroscience · doi:10.1016/s0306-4522(02)00306-8 · Cited by 78 · see citing works
Blast TBI models, neuropathology, and implications for seizure risk.
Kovacs, S. K., Leonessa, F., & Ling, G. S. F. (2014) · Frontiers in Neurology · doi:10.3389/fneur.2014.00047 · Cited by 71 · see citing works
Origin of the Red-Shifted Optical Spectra Recorded for Aza-BODIPY Dyes
Karlsson, J. K. G., & Harriman, A. (2016) · The Journal of Physical Chemistry A · doi:10.1021/acs.jpca.6b01278 · Cited by 68 · see citing works
Vigil, F. A., Bozdemir, E., Bugay, V., Chun, S. H., Hobbs, M., Sanchez, I., Hastings, S. D., Veraza, R. J., Holstein, D. M., Sprague, S. M., et al. (2020) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1177/0271678x19857818 · Cited by 51 · see citing works
Cippitelli, A., Zook, M., Bell, L., Damadzic, R., Eskay, R. L., Schwandt, M., & Heilig, M. (2010) · Neurobiology of Learning and Memory · doi:10.1016/j.nlm.2010.09.006 · Cited by 44 · see citing works
Hwang, I. K., Lee, C. H., Li, H., Yoo, K.-Y., Choi, J. H., Kim, D. W., Kim, D.-W., Suh, H.-W., & Won, M.-H. (2008) · Neurochemical Research · doi:10.1007/s11064-007-9584-6 · Cited by 40 · see citing works
Kaur, D., Rajagopalan, S., Chinta, S., Kumar, J., Di Monte, D., Cherny, R. A., & Andersen, J. K. (2007) · Brain Research · doi:10.1016/j.brainres.2006.03.006 · Cited by 36 · see citing works
Unsain, N., Montroull, L. E., & Mascó, D. H. (2009) · Journal of Neurochemistry · doi:10.1111/j.1471-4159.2009.06342.x · Cited by 32 · see citing works
Ohk, T. G., Yoo, K.-Y., Park, S. M., Shin, B. N., Kim, I. H., Park, J. H., Ahn, H. C., Lee, Y. J., Kim, M. J., Kim, T. Y., et al. (2012) · Neurochemical Research · doi:10.1007/s11064-011-0678-9 · Cited by 29 · see citing works
Jin, J., Kang, H.-M., Jung, J., Jeong, J.-W., & Park, C. (2014) · NeuroReport · doi:10.1097/wnr.0000000000000046 · Cited by 21 · see citing works
- Authors
- Schmued, L. C., & Hopkins, K. J.
- Affiliations
- National Center for Toxicological Research (US)
- Corresponding author
- Schmued, Larry C
- ORCID
- Schmued, Larry C
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 2000-08
- Volume
- 874
- Issue
- 2
- Pages
- 123-130
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W2081319561
- Cited by
- 1,218
- References
- 6
- Citations by year
- 2026: 11 · 2025: 9 · 2024: 13 · 2023: 25 · 2022: 24 · 2021: 35 · 2020: 35 · 2019: 46 · 2018: 39 · 2017: 49 · 2016: 53 · 2015: 56 · 2014: 52 · 2013: 70 · 2012: 84
- FWCI
- 16.63
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, public-domain)
- Funders
- Oak Ridge Institute for Science and Education
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Keywords
- Fluoro-Jade B, neurodegeneration, fluorescent staining, signal-to-noise ratio, dendrites, axons, axon terminals
- MeSH terms
- Animals, Brain, Fluoresceins, Fluorescent Antibody Technique, Fluorescent Dyes, Kainic Acid, Male, Nerve Degeneration, Neurons, Staining and Labeling, Rats, Sprague-Dawley, Rats
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