Cited by 1,218Advanced Fluorescence Microscopy TechniquesNeuroscience and Neuropharmacology ResearchNeuroinflammation and Neurodegeneration Mechanisms

Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration

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Schmued, Larry C, Hopkins, Keri J·2000·Brain Research

Abstract

Fluoro-Jade B, like its predecessor Fluoro-Jade, is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. However, Fluoro-Jade B has an even greater specific affinity for degenerating neurons. This notion is supported by the conspicuous staining of degenerating neuronal elements with minimal background staining. This improved signal-to-noise ratio means that fine neuronal processes including distal dendrites, axons and axon terminals can be more readily detected and documented. Although the staining time and dye concentration are reduced, the method is as rapid, simple and reliable as the original Fluoro-Jade technique. Like Fluoro-Jade, Fluoro-Jade B is compatible with a number of other labeling procedures including immunofluorescent and fluorescent Nissl techniques.

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Authors
Schmued, L. C., & Hopkins, K. J.
Affiliations
National Center for Toxicological Research (US)
Corresponding author
Schmued, Larry C
Venue
Brain Research
Publisher
Elsevier BV
Published
2000-08
Volume
874
Issue
2
Pages
123-130
ISSN
0006-8993 (ISSN-L)
OpenAlex
W2081319561
Cited by
1,218
References
6
Citations by year
2026: 11 · 2025: 9 · 2024: 13 · 2023: 25 · 2022: 24 · 2021: 35 · 2020: 35 · 2019: 46 · 2018: 39 · 2017: 49 · 2016: 53 · 2015: 56 · 2014: 52 · 2013: 70 · 2012: 84
FWCI
16.63
Type
journal article
Language
en
Open access
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
  • Zenodo (CERN European Organization for Nuclear Research) (submittedVersion, public-domain)
Funders
Oak Ridge Institute for Science and Education
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
Keywords
Fluoro-Jade B, neurodegeneration, fluorescent staining, signal-to-noise ratio, dendrites, axons, axon terminals
MeSH terms
Animals, Brain, Fluoresceins, Fluorescent Antibody Technique, Fluorescent Dyes, Kainic Acid, Male, Nerve Degeneration, Neurons, Staining and Labeling, Rats, Sprague-Dawley, Rats

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