Cited by 137RNA Interference and Gene DeliveryNanoparticle-Based Drug DeliveryAdvancements in Transdermal Drug Delivery

Mannan-Modified Solid Lipid Nanoparticles for Targeted Gene Delivery to Alveolar Macrophages

Yu, Wangyang, Liu, Chunxi, Liu, Yu, Zhang, Na, Xu, Wenfang·2010·Pharmaceutical Research

Abstract

Purpose Cationic solid lipid nanoparticles (SLN) have established themselves during the past decades. They can efficiently bind DNA directly via ionic interaction and mediate gene transfection. One major problem with SLN is the lack of cell-targeting ability. In the present study, a mannan-based PE-grafted ligand was synthesized and used for the surface modification of DNA-loaded cationic SLN to prepare Man-SLN-DNA.Methods For in vitro test, the cytotoxicity and transfection investigation was carried out on murine macrophage cell line RAW 264.7. For in vivo evaluation, Man-SLN-DNA was delivered into the lung of the rats, and the alveolar macrophages (AM) were isolated for the fluorescence determination of transfection efficiency.Results When compared with non-modified SLN-DNA and Lipofectamine 2000-DNA, Man-SLN-DNA produced the highest gene expressions, especially in vivo.Conclusion These results demonstrated the active targeting ability of this kind of mannan-modified DNA-loaded vehicles, which may have great potential for targeted gene delivery.

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Crossref · 10.1007/s11095-010-0149-z

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Authors
Yu, W., Liu, C., Liu, Y., Zhang, N., & Xu, W.
Affiliations
Shandong University (CN)
Corresponding author
Zhang, Na
Venue
Pharmaceutical Research
Published
2010-04-27
Volume
27
Issue
8
Pages
1584-1596
ISSN
  • 0724-8741 (ISSN-L)
  • 1573-904X
OpenAlex
W2008296427
Cited by
137
References
39
Citations by year
2026: 1 · 2025: 3 · 2024: 7 · 2023: 8 · 2022: 15 · 2021: 9 · 2020: 14 · 2019: 8 · 2018: 6 · 2017: 7 · 2016: 11 · 2015: 10 · 2014: 10 · 2013: 7 · 2012: 9
FWCI
3.64
Type
journal article
Language
en
Open access
closed
Locations
  • Pharmaceutical Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advancements in Transdermal Drug Delivery
Keywords
solid lipid nanoparticles, mannan, cationic liposomes, gene delivery, alveolar macrophages, targeted gene delivery, surface modification, DNA transfection, pulmonary gene delivery, RAW 264.7 macrophages
MeSH terms
Animals, Cell Line, Cell Survival, Concanavalin A, Ligands, Lipids, Male, Mannans, Phosphatidylethanolamines, Genetic Therapy, Molecular Structure, Drug Delivery Systems, Macrophages, Alveolar, Rats, Wistar, Cell Line, Tumor, Microscopy, Electron, Transmission, Mice, Rats, Nanoparticles

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