Combined use of Multiple Methodologies for the Measurement of Total Antioxidant Capacity in UK Commercially Available Vegetable Juices
Abstract
Substantial evidence exists to support the hypothesis that high fruit and vegetable consumption, rich in antioxidants, can reduce the incidence of several disease states. The aim of this study was to compare the results obtained by six spectrophotometric biochemical methods including the ferric reducing antioxidant power (FRAP), 2, 2-diphenyl-1-picryhydrazyl (DPPH•), 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS•⁺), copper (II) reducing capacity (CUPRAC) and Cerium (IV) reducing antioxidant capacity (CERAC) assays as well as Folin-Ciocalteu method (FC) for the measurement of total antioxidant capacity (TAC) and total polyphenols (TP) in different commercially available vegetable juices. There was a significant positive correlation between the results obtained for FRAP, ABTS•⁺, CUPRAC, CERAC and FC (0.68 ≤ r ≤ 0.96, P < 0.01). DPPH• was only correlated with CERAC (r = 0.66, P < 0.01). Beetroot juice had the highest TAC and TP regardless of the method of analysis.
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Beetroot juice contains other compounds that may be independently bioactive or act synergistically with nitrate, including antioxidants (betaine and vitamins) and polyphenols, resveratrol and quercetin.
“In addition to nitrate, beetroot juice also contains other compounds that may be independently bioactive or that may act synergistically with nitrate, including antioxidants (betaine and vitamins) and the polyphenols, resveratrol and quercetin [45].”
From Dietary Nitrate Supplementation and Exercise Performance (2014) · cites this paper as [45]
Any possible harmful effect of nitrosation is very effectively inhibited by the antioxidants that accompany nitrate in vegetables.
“It is pertinent to note that any possible harmful effect of nitrosation (which assumes the presence of secondary amines in the saliva or stomach) is very effectively inhibited [77] by the antioxidants that accompany nitrate in vegetables [45].”
From Dietary Nitrate Supplementation and Exercise Performance (2014) · cites this paper as [45]
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Dietary Nitrate Supplementation and Exercise Performance
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- Authors
- Wootton-Beard, P. C., & Ryan, L.
- Affiliations
- Oxford Brookes University (GB)
- Corresponding author
- Ryan, Lisa
- Venue
- Plant Foods for Human Nutrition
- Published
- 2012-04-27
- Volume
- 67
- Issue
- 2
- Pages
- 142-147
- ISSN
- 0033-5134 (ISSN-L)
- 0921-9668
- 1573-9104
- OpenAlex
- W1965270519
- Cited by
- 25
- References
- 35
- Citations by year
- 2024: 3 · 2023: 2 · 2022: 3 · 2021: 1 · 2019: 4 · 2018: 1 · 2017: 2 · 2016: 2 · 2015: 3 · 2014: 1 · 2013: 3
- FWCI
- 1.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Plant Foods for Human Nutrition (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food and Agricultural Sciences
- Keywords
- total antioxidant capacity, total polyphenols, vegetable juice, commercial fruit juices, FRAP, DPPH radical scavenging, ABTS radical scavenging, CUPRAC, Folin-Ciocalteu assay, beetroot juice, fruit and vegetable intake
- MeSH terms
- Antioxidants, Beverages, Biphenyl Compounds, Chromans, United Kingdom, Picrates, Spectrophotometry, Sulfonic Acids, Vegetables, Fluorescence Recovery After Photobleaching, Benzothiazoles, Polyphenols
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