Somatic Embryogenesis in Vaccinium vitis-Idaea L.: A High-Fidelity Micropropagation Approach Producing True-to-Type Plants with Enhanced Phytochemical Profiles
Open accessAbstract
The “superfruit” lingonberry ( Vaccinium vitis-idaea L.) is well known for its enormous health benefits and rich phytochemical contents. However, its regeneration and propagation face significant challenges due to the woody nature of the plant and poor proliferation rates using conventional methods. By using juvenile leaf explants of two V. vitis-idaea genotypes, this study introduces a novel and efficient somatic embryogenesis (SE) protocol for lingonberry micropropagation. The berry basal medium supplemented with 5.5 µM thidiazuron was optimized for the SE protocol where 92% embryogenic callus induction was achieved. The effectiveness of plantlet regeneration was greatly increased by employing 4.0 µM zeatin. Scanning electron microscopy study and histological analyses confirmed the development, maturation, and structural integrity of somatic embryos. Clonal fidelity study between the donor and regenerated plants by expressed sequence tag-simple sequence repeat (EST-SSR) and genomic simple sequence repeat (GSSR) ensured the genetic stability and maintenance of trueness-to-type of the SE micropropagules. The SE-regenerated plants showed higher levels of flavonoids, anthocyanins, and antioxidant activity than donor plants, indicating their improved nutritional and medicinal potential. Furthermore, SE-regenerated plants possessed elevated levels of antioxidant enzymes like catalase and superoxide dismutase, which enhanced their ability to withstand stress. The findings from this study provide a scalable, reliable, and sustainable approach to lingonberry micropropagation, combining genetic fidelity with enhanced phytochemical profiles.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Kundu et al. developed a high-fidelity somatic embryogenesis protocol in Vaccinium vitis-idaea; regenerated plants retained genetic uniformity and showed increased flavonoids, anthocyanins, and antioxidant activity compared to donor plants.
“Kundu et al. developed a high-fidelity somatic embryogenesis protocol in Vaccinium vitis-idaea (lingonberry).This study revealed that the regenerated plants retained genetic uniformity while also showing increased concentrations of flavonoids, anthocyanins, and antioxidant activity in comparison to the original donor plants [17].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [17]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
The number of known plants species in the world and its annual increase
Christenhusz, M. J. M., & Byng, J. W. (2016) · Phytotaxa · doi:10.11646/phytotaxa.261.3.1 · Cited by 2,128 · see citing works
Oxygen Stress and Superoxide Dismutases
Scandalios, J. G. (1993) · PLANT PHYSIOLOGY · doi:10.1104/pp.101.1.7 · Cited by 1,700 · see citing works
Somatic Embryogenesis: A Model for Early Development in Higher Plants
Zimmerman, J. L. (1993) · The Plant Cell · doi:10.1105/tpc.5.10.1411 · Cited by 834 · see citing works
The cell biology of lignification in higher plants
Barros, J., Serk, H., Granlund, I., & Pesquet, E. (2015) · Annals of Botany · doi:10.1093/aob/mcv046 · Cited by 731 · see citing works
Antioxidant defense responses: physiological plasticity in higher plants under abiotic constraints
Jaleel, C. A., Ksouri, R., Gopi, R., Manivannan, P., Jallali, I., Aljuburi, H. J., Zhao, C., Shao, H., & Panneerselvam, R. (2009) · Acta Physiologiae Plantarum · doi:10.1007/s11738-009-0275-6 · Cited by 479 · see citing works
Verma, S., & Mishra, S. N. (2005) · Journal of Plant Physiology · doi:10.1016/j.jplph.2004.08.008 · Cited by 402 · see citing works
JMP statistical discovery software
Jones, B., & Sall, J. (2011) · Wiley Interdisciplinary Reviews Computational Statistics · doi:10.1002/wics.162 · Cited by 290 · see citing works
Cytokinins: Biosynthesis metabolism and perception
Mok, M. C., Martin, R. C., & Mok, D. W. S. (2000) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-000-0021-7 · Cited by 125 · see citing works
Antioxidant Enzyme Activities and Abiotic Stress Tolerance Relationship in Vegetable Crops
Kuşvuran, Ş., KIRAN, S., & Ellialtıoğlu, Ş. Ş. (2016) · InTech eBooks · doi:10.5772/62235 · Cited by 123 · see citing works
Localization of Fluoride and Aluminum in Subcellular Fractions of Tea Leaves and Roots
Gao, H., Zhao, Q., Zhang, X., Wan, X., & Mao, J. (2014) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf4038437 · Cited by 110 · see citing works
Kowalska, K. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22105126 · Cited by 100 · see citing works
Raudonė, L., Vilkickytė, G., Pitkauskaitė, L., Raudonis, R., Vainorienė, R., & Motiekaitytė, V. (2019) · Molecules · doi:10.3390/molecules24050844 · Cited by 75 · see citing works
Somatic Embryogenesis Induction in Woody Species: The Future After OMICs Data Assessment
Pais, M. S. (2019) · Frontiers in Plant Science · doi:10.3389/fpls.2019.00240 · Cited by 75 · see citing works
Locatelli, C., Nardi, G. M., anuário, A. D. F., Freire, C., Megiolaro, F., Schneider, K., Perazzoli, M. A., Nascimento, S. D., Gon, A., Mariano, L. N. B., et al. (2016) · Pharmacognosy Research · doi:10.4103/0974-8490.178642 · Cited by 75 · see citing works
Debnath, S. C., & McRae, K. B. (2001) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-001-0043-9 · Cited by 70 · see citing works
Self-citationGhosh, A., Igamberdiev, A. U., & Debnath, S. C. (2018) · Scientific Reports · doi:10.1038/s41598-018-35233-6 · Cited by 70 · see citing works
Self-citationVilkickytė, G., Raudonė, L., & Petrikaitė, V. (2020) · Antioxidants · doi:10.3390/antiox9121261 · Cited by 70 · see citing works
In vitro propagation and secondary product production by Merwilla plumbea (Lindl.) Speta
Baskaran, P., Ncube, B., & Staden, J. V. (2012) · Plant Growth Regulation · doi:10.1007/s10725-012-9682-6 · Cited by 65 · see citing works
Fraga, H. P. D. F., Vieira, L. D. N., Heringer, Â. S., Puttkammer, C. C., Silveira, V., & Guerra, M. P. (2016) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-016-0956-y · Cited by 63 · see citing works
Samad, N. B., Debnath, T., Ye, M., Hasnat, M. A., & Lim, B. O. (2014) · Asian Pacific Journal of Tropical Biomedicine · doi:10.12980/apjtb.4.2014c1008 · Cited by 61 · see citing works
Debnath, S. C., & Goyali, J. C. (2020) · Molecules · doi:10.3390/molecules25040788 · Cited by 60 · see citing works
Self-citationA Scale-up System for Lowbush Blueberry Micropropagation Using a Bioreactor
Debnath, S. C. (2009) · HortScience · doi:10.21273/hortsci.44.7.1962 · Cited by 59 · see citing works
Self-citationSharma, S., & Millam, S. (2004) · Plant Cell Reports · doi:10.1007/s00299-004-0814-y · Cited by 58 · see citing works
Debnath, S. C., & Arigundam, U. (2020) · Agronomy · doi:10.3390/agronomy10050744 · Cited by 55 · see citing works
Self-citationBioreactors and Molecular Analysis in Berry Crop Micropropagation—A Review
Debnath, S. (2011) · Canadian Journal of Plant Science · doi:10.4141/cjps10131 · Cited by 47 · see citing works
Self-citationKhan, A., Shah, A. H., & Ali, N. (2021) · Scientific Reports · doi:10.1038/s41598-021-03032-1 · Cited by 44 · see citing works
SOMATIC EMBRYOGENESIS AND EMBRYO MATURATION IN PERSIAN WALNUT
Vahdati, K., Jariteh, M., Niknam, V., Mirmasoumi, M., & Ebrahimzadeh, H. (2005) · Acta Horticulturae · doi:10.17660/actahortic.2005.705.24 · Cited by 43 · see citing works
Oxidative stress in recalcitrant tissue cultures of grapevine
Benson, E. E., & Roubelakis-Angelakis, K. A. (1994) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(94)90037-x · Cited by 40 · see citing works
Sadat‐Hosseini, M., Vahdati, K., & Leslie, C. A. (2019) · HortScience · doi:10.21273/hortsci13787-18 · Cited by 39 · see citing works
Direct somatic embryogenesis in Epipactis veratrifolia, a temperate terrestrial orchid
Moradi, S., Daylami, S. D., Arab, M., & Vahdati, K. (2016) · The Journal of Horticultural Science and Biotechnology · doi:10.1080/14620316.2016.1228434 · Cited by 38 · see citing works
Effect of TDZ on Various Plant Cultures
Pai, S. R., & Desai, N. S. (2018) · Springer eBooks · doi:10.1007/978-981-10-8004-3_25 · Cited by 37 · see citing works
Improved shoot organogenesis from hypocotyl segments of lingonberry (Vaccinium vitis-idaea L.)
Debnath, S. C. (2003) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1079/ivp2003458 · Cited by 34 · see citing works
Self-citationGoyali, J. C., Igamberdiev, A. U., & Debnath, S. C. (2015) · HortScience · doi:10.21273/hortsci.50.6.888 · Cited by 34 · see citing works
Self-citationDebnath, S. C. (2016) · The Journal of Horticultural Science and Biotechnology · doi:10.1080/14620316.2016.1224606 · Cited by 34 · see citing works
Self-citationBhattacharya, S., Bandopadhyay, T. K., & Ghosh, P. (2009) · Scientia Horticulturae · doi:10.1016/j.scienta.2009.10.011 · Cited by 33 · see citing works
Goyali, J. C., Igamberdiev, A. U., & Debnath, S. C. (2013) · Canadian Journal of Plant Science · doi:10.4141/cjps2012-307 · Cited by 32 · see citing works
Self-citationDebnath, S. C. (2011) · Scientia Horticulturae · doi:10.1016/j.scienta.2011.01.012 · Cited by 31 · see citing works
Self-citationRadhakrishnan, R., Ramachandran, A., & Kumari, B. D. R. (2009) · Acta Physiologiae Plantarum · doi:10.1007/s11738-009-0356-6 · Cited by 29 · see citing works
Phenotypic variation and epigenetic insight into tissue culture berry crops
Debnath, S. C., & Ghosh, A. (2022) · Frontiers in Plant Science · doi:10.3389/fpls.2022.1042726 · Cited by 27 · see citing works
Self-citationANTIOXIDANT POTENTIAL AND OXIDATIVE DNA DAMAGE PREVENTIVE ACTIVITY OFCHRYSANTHEMUM INDICUMEXTRACTS
Debnath, T., Jin, H. L., Hasnat, M. A., 김윤숙, Samad, N. B., Park, P., & Lim, B. O. (2012) · Journal of Food Biochemistry · doi:10.1111/j.1745-4514.2011.00644.x · Cited by 26 · see citing works
Debnath, S. C. (2005) · HortScience · doi:10.21273/hortsci.40.3.760 · Cited by 24 · see citing works
Self-citationVyas, P., Debnath, S. C., & Igamberdiev, A. U. (2013) · Biologia Plantarum · doi:10.1007/s10535-013-0339-8 · Cited by 22 · see citing works
Self-citationOliveira, L. B., de Mello, T., de Araújo, C. P., de Oliveira, J. P. B., Ferreira, A., Zanardo, T. É. C., Vieira, L. M., Otoni, W. C., Alexandre, R. S., & Carvalho, V. S. (2022) · Scientia Horticulturae · doi:10.1016/j.scienta.2022.111051 · Cited by 21 · see citing works
Epigenomic insight of lingonberry and health-promoting traits during micropropagation
Sikdar, A., Sharma, U., Barua, R., Igamberdiev, A. U., & Debnath, S. C. (2022) · Scientific Reports · doi:10.1038/s41598-022-16530-7 · Cited by 20 · see citing works
Self-citationBarua, R., Kundu, S., Majumder, S., Marshall, H. D., Igamberdiev, A. U., & Debnath, S. C. (2025) · Plant Growth Regulation · doi:10.1007/s10725-025-01293-8 · Cited by 13 · see citing works
Self-citationCHAVEZ-CORTAZAR, A., MATA-ROSAS, M., OYAMA, K., SAMAIN, M. S., & QUESADA, M. (2020) · Biologia plantarum · doi:10.32615/bp.2020.006 · Cited by 12 · see citing works
Elmoreigi, R. A., El-Maaty, S. A., Hassanen, S., & Hussein, E. H. A. (2024) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-024-02927-x · Cited by 12 · see citing works
Long, X., Zhang, J., Wang, D., Weng, Y., Liu, S., Li, M., Hao, Z., Cheng, T., Shi, J., & Chen, J. (2023) · Forestry Research · doi:10.48130/fr-2023-0015 · Cited by 12 · see citing works
Secondary somatic embryogenesis in Cork oak: influence of plant growth regulators
Ali, N. B., Benkaddour, R., Rahmouni, S., Boussaoudi, I., Hamdoun, O., Hassoun, M., Azaroual, L., Badoc, A., Martin, P., & Lamarti, A. (2023) · Forest Science and Technology · doi:10.1080/21580103.2023.2172462 · Cited by 10 · see citing works
Thidiazuron: A Potent Phytohormone for In Vitro Regeneration
Govindaraj, S. (2018) · Springer eBooks · doi:10.1007/978-981-10-8004-3_22 · Cited by 10 · see citing works
Bhat, A. Y., Shahzad, A., Kausar, A., & Rashid, A. (2024) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-024-02937-9 · Cited by 5 · see citing works
Saleh, E. O. L., Luís, Z. G., Silva-Cardoso, I. M. D. A., & Scherwinski-Pereira, J. E. (2024) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-024-02884-5 · Cited by 5 · see citing works
Flagged first, then by citations
Research works printed but not linked: 2
- 1 · DOI
- Jariteh M, Ebrahimzadeh H, Niknam V, Vahdati K, Amiri R (2011). Antioxidant enzymes activities during secondary somatic embryogenesis in Persian walnut (Juglans regia L.). African Journal of Biotechnology, 10(20), 4093-4099. h t t p s : / / d o i . o r g / 1 0 . 2 1 2 7 3 / H O R T S C I 1 3 7 8 7 -1 8 · DOI
Cited by
Sorted by citations
Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives
Majumder, S., Igamberdiev, A. U., & Debnath, S. C. (2025) · Agronomy · doi:10.3390/agronomy15061489 · Cited by 46 · see citing works
Self-citationMajumder, S., Igamberdiev, A. U., & Debnath, S. C. (2025) · Plant Methods · doi:10.1186/s13007-025-01471-9 · Cited by 4 · see citing works
Self-citationSikdar, A., Igamberdiev, A. U., Sun, S., & Debnath, S. C. (2025) · Smart Agricultural Technology · doi:10.1016/j.atech.2025.101388 · Cited by 4 · see citing works
Self-citationHyperhydricity Syndrome in In Vitro Plants: Mechanisms, Physiology, and Control
Barua, R., Igamberdiev, A. U., & Debnath, S. C. (2025) · Plants · doi:10.3390/plants14243721 · Cited by 3 · see citing works
Self-citationKundu, S., Majumder, S., Barua, R., Marshall, H. D., Igamberdiev, A. U., & Debnath, S. C. (2026) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-025-10625-2 · Cited by 2 · see citing works
Self-citationEpigenetic variations in micropropagated horticultural crops and their prospects in crop improvement
Debnath, S. C. (2026) · Acta Horticulturae · doi:10.17660/actahortic.2026.1454.12
Self-citationKiełkiewicz, R. M., Śliwińska, A. A., Tomiczak, K., Tryngiel-Gać, A., Ostrowska, A., Sobczak, M., & Obrębski, M. (2026) · Industrial Crops and Products · doi:10.1016/j.indcrop.2026.123427
Kundu, S., Majumder, S., Barua, R., Marshall, H. D., Igamberdiev, A. U., & Debnath, S. C. (2026) · Planta · doi:10.1007/s00425-026-05073-0
Self-citationChang, Y., Xu, K., Wang, D., Xie, X., 陶芳芝, Zhang, S., Ma, S., Cheng, Q., Chen, Y., Wang, J., et al. (2026) · Scientific Reports · doi:10.1038/s41598-026-63249-w
Synthetic seeds in black huckleberry: clonal fidelity and oxidative balance assessment
Majumder, S., Kundu, S., Barua, R., Igamberdiev, A. U., & Debnath, S. C. (2026) · Plant Growth Regulation · doi:10.1007/s10725-026-01497-6
Self-citationBhasin, S., Mashakhetri, K. D., Sen, Y., Srinivas, K., & Vyas, D. (2026) · Plant Cell Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-026-03571-3
Nicuţă, D., Grosu, L., Voicu, R.-E., & ALEXA, I.-C. (2026) · Horticulturae · doi:10.3390/horticulturae12091120
Jogam, P., Rohela, G. K., Aileni, M., Sandhya, D., Singisala, N. R., & Peddaboina, V. (2026) · Elsevier eBooks · doi:10.1016/b978-0-443-43944-5.00008-6
Yadav, S., Singh, A., Rathour, R., & Manoj, K. (2026) · In Vitro Cellular & Developmental Biology - Plant · doi:10.1007/s11627-026-10712-y
- Authors
- Kundu, S., Barua, R., Marshall, H. D., Siow, Y. L., Igamberdiev, A. U., & Debnath, S. C.
- Affiliations
- Memorial University of Newfoundland (CA)
- Agriculture and Agri-Food Canada (CA)
- Corresponding author
- Debnath, Samir C.
- Venue
- Plant Cell, Tissue and Organ Culture (PCTOC)
- Published
- 2025-04-25
- Volume
- 161
- Issue
- 2
- ISSN
- 0167-6857 (ISSN-L)
- 1573-5044
- OpenAlex
- W4409815352
- Cited by
- 14
- References
- 52
- Citations by year
- 2026: 10 · 2025: 4
- FWCI
- 4.21
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Plant Cell Tissue and Organ Culture (PCTOC) (publishedVersion, cc-by)
- Funders
- Agriculture & Agri-Food Canada
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Berry genetics and cultivation research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Keywords
- somatic embryogenesis, lingonberry, micropropagation, thidiazuron, zeatin, clonal fidelity, phytochemical content, flavonoids, anthocyanins, antioxidant activity, antioxidant enzymes, embryogenic callus, woody plant regeneration
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
