Induction of Somatic Embryogenesis and Evaluation of Genetic Stability in Regenerated Plants of Magnolia Dealbata
Open accessAbstract
The utility of plant tissue culture for the mass propagation of trees is well known, but continuous in vitro multiplication of plant material may increase the possibility of somaclonal variation; therefore, it is essential to evaluate the genetic integrity of regenerants from species-specific in vitro protocols prior to mass production and implementation. The objectives of this study were: 1) to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) concentration over two cycles of secondary somatic embryogenesis in Magnolia dealbata; and 2) to verify the genetic stability of the regenerants obtained. The embryogenic response was not significantly affected by the concentration of 2,4-D but did vary across cycles of induction. The addition of 4.52 μM 2,4-D induced the highest total number of embryos (100.5), the mean number of somatic embryos (25.1) and somatic embryos per explant (80.6). In both 2,4-D concentration (2.26 or 4.52 μM), genetic integrity between the donor and the propagated clones was 0.90, and the low genetic instability (≤ 0.10 in both PGR treatments) might be due to effect of cyclic somatic embryogenesis or the different response of the explants at stress in in vitro culture conditions. However, it is necessary to examine more cell lines and somatic embryogenesis cycles.
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In Magnolia dealbata, genetic integrity was 90%.
“Genetic integrity 90%.”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [87]
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- Authors
- CHAVEZ-CORTAZAR, A., MATA-ROSAS, M., OYAMA, K., SAMAIN, M. S., & QUESADA, M.
- Affiliations
- Instituto de Ecología (MX)
- Venue
- Biologia plantarum
- Publisher
- Institute of Experimental Botany
- Published
- 2020-03-11
- Volume
- 64
- Pages
- 224-233
- ISSN
- 0006-3134 (ISSN-L)
- 1573-8264
- OpenAlex
- W3012207813
- Cited by
- 12
- References
- 68
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 3
- FWCI
- 0.57
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by-nc-nd
- Locations
- Biologia Plantarum (publishedVersion, cc-by-nc-nd)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Consejo Nacional de Ciencia y Tecnología
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- Magnolia dealbata, somatic embryogenesis, genetic stability, somaclonal variation, 2,4-D, secondary somatic embryogenesis, plant tissue culture, regenerated plants, plant genetic resources
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