Bioreactors and Molecular Analysis in Berry Crop Micropropagation—A Review
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Debnath, S. C. 2011. Bioreactors and molecular analysis in berry crop micropropagation – A review. Can. J. Plant Sci. 91: 147–157. While berry fruits have long enjoyed huge popularity among consumers, tremendous progress in plant tissue culture, resulting in great advances in micropropagation, has occurred. Of particular significance has been the evolution of the technology permitting multiplication of berry plants in bioreactors containing liquid media. Although automation of micropropagation in bioreactors has been advanced as a possible way of reducing propagation cost, optimal plant production depends upon better understanding of physiological and biochemical responses of plant to the signals of culture microenvironment and an optimization of specific physical and chemical culture conditions to control the morphogenesis of berry plants in liquid culture systems. Clonal fidelity can be a serious problem, and molecular strategies have been developed in order to reduce the variation to manageable levels. Molecular markers have been introduced to tissue culture research and can potentially be used in various facets of pertinent studies with berry crops. The paper focuses on bioreactor systems combined with semi-solid media used for in vitro culture of berry crops, cultivation of micropropagules and employment of molecular markers in micropropagated plants for the assessment of genetic fidelity, uniformity, stability and trueness-to-type among donor plants and tissue culture regenerants. The pertinent literature is reviewed and the relative merits and shortcomings of the various molecular markers applied are presented with an emphasis on the nature of tissue culture-induced variation.
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Bioreactor systems have demonstrated potential in reducing the frequency of somaclonal variation by providing uniform and controlled culture environments.
“Bioreactor systems have also demonstrated potential in reducing the frequency of somaclonal variation by providing uniform and controlled culture environments [16].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [16]
The BIOREACTORS section (page unavailable) describes control of culture conditions, and the CONCLUSIONS section (page unavailable) identifies cultural environment as a factor in somaclonal variation. Neither passage demonstrates that bioreactors reduce the frequency of somaclonal variation; the reported genetic-fidelity comparison does not show a reduction relative to gelled culture. (LLM, conf=0.84)
Experimental — accuracy not yet measuredThe bioreactor system provides a controlled environment that provides consistent growth conditions for plant tissues, thereby minimizing somaclonal variation.
“The bioreactor system provides a controlled environment that provides consistent growth conditions for plant tissues, thereby minimizing somaclonal variation [16,82].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [16]
The BIOREACTORS section (page unavailable) describes bioreactors as controlled environments in which temperature, gases, and pH can be monitored. The CONCLUSIONS section (page unavailable) lists cultural environment among factors affecting somaclonal variation, but does not say that bioreactors provide consistent growth conditions that minimize that variation. (LLM, conf=0.85)
Experimental — accuracy not yet measuredBioreactor-derived strawberry plants showed genetic uniformity and had enhanced anthocyanin content and antioxidant activity compared to conventionally propagated plants.
“Similarly, confirmed genetic uniformity in bioreactor-derived strawberry (Fragaria × ananassa) plants and correlated clonal fidelity with enhanced anthocyanin content and antioxidant activity compared to conventionally propagated plants [16].”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [16]
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- Authors
- Debnath, S.
- Affiliations
- Agriculture and Agri-Food Canada (CA)
- Corresponding author
- Debnath, Samir
- ORCID
- Debnath, Samir
- Venue
- Canadian Journal of Plant Science
- Publisher
- Canadian Science Publishing
- Published
- 2011-01
- Volume
- 91
- Issue
- 1
- Pages
- 147-157
- ISSN
- 0008-4220 (ISSN-L)
- 1918-1833
- OpenAlex
- W2141144606
- Cited by
- 47
- References
- 85
- Citations by year
- 2026: 6 · 2025: 3 · 2024: 2 · 2023: 6 · 2022: 5 · 2021: 5 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 1
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Update notices
- correction (2015-07-01)
- Open access
- bronze
- doi.org
- Locations
- Canadian Journal of Plant Science (publishedVersion)
- BioOne Complete (BioOne) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Berry genetics and cultivation research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Horticultural and Viticultural Research
- Keywords
- berry crops, micropropagation, bioreactors, liquid culture, clonal fidelity, genetic fidelity, molecular markers, morphogenesis
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