The GC-MS metabolomics signature in patients with fibromyalgia syndrome directs to dysbiosis as an aspect contributing factor of FMS pathophysiology

Malatji, Bontle G.; Mason, Shayne; Mienie, Lodewyk J.; Wevers, Ron A.; Meyer, Helgard; van Reenen, Mari; Reinecke, Carolus J.

doi:10.1007/s11306-019-1513-6

Cited by 48Fibromyalgia and Chronic Fatigue Syndrome ResearchMetabolomics and Mass Spectrometry StudiesGut microbiota and health

The GC-MS metabolomics signature in patients with fibromyalgia syndrome directs to dysbiosis as an aspect contributing factor of FMS pathophysiology

Malatji, Bontle G., Mason, Shayne, Mienie, Lodewyk J., Wevers, Ron A., Meyer, Helgard, van Reenen, Mari, Reinecke, Carolus J.·2019·Metabolomics

DOI 10.1007/s11306-019-1513-6

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Abstract

Introduction Fibromyalgia syndrome (FMS) is a chronic pain syndrome. Previous analyses of untargeted metabolomics data indicated altered metabolic profile in FMS patients.Objectives We report a semi-targeted explorative metabolomics study on the urinary metabolite profile of FMS patients; exploring the potential of urinary metabolite information to augment existing medical diagnosis.Methods All cases were females. Patients had a medical history of persistent FMS (n = 18). Control groups were first-generation family members of the patients (n = 11), age-related individuals without indications of FMS (n = 10), and healthy, young (18-22 years) individuals (n = 41). The biofluid investigated was early morning urine samples. Data generation was done through gas chromatography-mass spectrometry (GC-MS) analysis and data processing and analyses were performed using Matlab, R, SPSS and SAS software.Results Quantitative analysis revealed the presence of 196 metabolites. Unsupervised and supervised multivariate analyses distinguished all three control groups and the FMS patients, which could be related to 14 significantly increased metabolites. These metabolites are associated with energy metabolism, digestion and metabolism of carbohydrates and other host and gut metabolites.Conclusions Overall, urinary metabolite profiles in the FMS patients suggest: (1) energy utilization is a central aspect of this pain disorder, (2) dysbiosis seems to prevail in FMS patients, indicated by disrupted microbiota metabolites, supporting the model that microbiota may alter brain function through the gut-brain axis, with the gut being a gateway to generalized pain, and (3) screening of urine from FMS is an avenue to explore for adding non-invasive clinical information for diagnosis and treatment of FMS.

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Probiotics have been shown to improve cognitive symptoms and biochemical markers in participants with various dysbiosis-related disorders, including Alzheimer's disease, fibromyalgia, and irritable bowel syndrome.
“Probiotics have been shown to improve cognitive symptoms and biochemical markers in participants with various dysbiosis-related disorders, including Alzheimer’s disease (476), fibromyalgia (477, 478), and irritable bowel syndrome (IBS) (479).”
From Signalling cognition: The gut microbiota and hypothalamic-pituitary-adrenal axis (2023) · cites this paper as [478]

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A diagnostic biomarker profile for fibromyalgia syndrome based on an NMR metabolomics study of selected patients and controls
Malatji, B. G., Meyer, H., Mason, S., Engelke, U. F. H., Wevers, R. A., van Reenen, M., & Reinecke, C. J. (2017) · BMC Neurology · doi:10.1186/s12883-017-0863-9 · Cited by 78 · see citing works
Self-citation
A metabolomics investigation of Fibromyalgia Syndrome
Malatji, B. G. (2018) · Boloka Institutional Repository (North-west University)
Self-citation
Nuclear Magnetic Resonance‐Based Metabolomic Profiling in Fibromyalgia Patients
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Machine learning identifies fatigue as a key symptom of fibromyalgia reflected in tyrosine, purine, pyrimidine, and glutaminergic metabolism
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Authors: Malatji, B. G., Mason, S., Mienie, L. J., Wevers, R. A., Meyer, H., van Reenen, M., & Reinecke, C. J.
Affiliations: North-West University (ZA)
Radboud University Medical Center (NL)
Kalafong Hospital (ZA)
Corresponding author: Mason, Shayne
ORCID: Malatji, Bontle G.
Mason, Shayne
Mienie, Lodewyk J.
Wevers, Ron A.
Meyer, Helgard
Venue: Metabolomics
Publisher: Springer Science and Business Media LLC
Published: 2019-03-27
Volume: 15
Issue: 4
Pages: 54-54
ISSN: 1573-3882 (ISSN-L)
1573-3890
DOI: 10.1007/s11306-019-1513-6
OpenAlex: W2923029396
Cited by: 48
References: 57
Citations by year: 2026: 3 · 2025: 8 · 2024: 3 · 2023: 9 · 2022: 5 · 2021: 7 · 2020: 11 · 2019: 2
FWCI: 5.00
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Metabolomics (publishedVersion)
PubMed (publishedVersion)
Radboud Repository (Radboud University) (submittedVersion)
UpSpace Institutional Repository (University of Pretoria) (submittedVersion)
Topics: Health Sciences › Medicine › Psychiatry and Mental health › Fibromyalgia and Chronic Fatigue Syndrome Research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
Keywords: fibromyalgia, gut dysbiosis, gut microbiota metabolites, gut-brain axis, energy metabolism, chronic pain, non-invasive diagnosis, metabolomics biomarkers
MeSH terms: Adult, Female, Fibromyalgia, Humans, Gas Chromatography-Mass Spectrometry, Middle Aged, Biomarkers, Multivariate Analysis, Metabolomics, Metabolome, Young Adult, Dysbiosis

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The GC-MS metabolomics signature in patients with fibromyalgia syndrome directs to dysbiosis as an aspect contributing factor of FMS pathophysiology

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