Aerobic Exercise Improves Reverse Cholesterol Transport in Cholesteryl Ester Transfer Protein Transgenic Mice
Abstract
We analyzed the effect of a 6‐week aerobic exercise training program on the in vivo macrophage reverse cholesterol transport (RCT) in human cholesteryl ester transfer protein (CETP) transgenic (CETP‐tg) mice. Male CETP‐tg mice were randomly assigned to a sedentary group or a carefully supervised exercise training group (treadmill 15 m/min, 30 min sessions, five sessions per week). The levels of plasma lipids were determined by enzymatic methods, and the lipoprotein profile was determined by fast protein liquid chromatography (FPLC). CETP activity was determined by measuring the transfer rate of 14 C‐cholesterol from HDL to apo‐B containing lipoproteins, using plasma from CETP‐tg mice as a source of CETP. The reverse cholesterol transport was determined in vivo by measuring the [3 H]‐cholesterol recovery in plasma and feces (24 and 48 h) and in the liver (48 h) following a peritoneal injection of [3 H]‐cholesterol labeled J774‐macrophages into both sedentary and exercise trained mice. The protein levels of liver receptors were determined by immunoblot, and the mRNA levels for liver enzymes were measured using RT‐PCR. Exercise training did not significantly affect the levels of plasma lipids or CETP activity. The HDL fraction assessed by FPLC was higher in exercise‐trained compared to sedentary mice. In comparison to the sedentary group, a greater recovery of [3 H]‐cholesterol from the injected macrophages was found in the plasma, liver and feces of exercise‐trained animals. The latter occurred even with a reduction in the liver CYP7A1 mRNA level in exercised trained animals. Exercise training increased the liver LDL receptor and ABCA‐1 protein levels, although the SR‐BI protein content was unchanged. The RCT benefit in CETP‐tg mice elicited by exercise training helps to elucidate the role of exercise in the prevention of atherosclerosis in humans.
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After 8 weeks, PCSK9 mRNA and SREBP2 levels were increased significantly in mice with high-fat diet and exercise training, 1.9 and 1.8 times higher than those only with high-fat diet.
“After 8 weeks, the levels of PCSK9 mRNA and sterol regulatory element binding protein 2 (SREBP2) were increased significantly in mice with high-fat diet and exercise training, 1.9 and 1.8 times higher than those only with high-fat diet respectively.”
From Effects of aerobic exercise on lipids and lipoproteins (2017) · cites this paper as [73]
Both plasma PCSK9 and cholesterol were reduced by 14% in mice with both high-fat diet and exercise training, while no change occurred in those only with high-fat diet.
“In addition, both plasma PCSK9 and cholesterol were reduced by 14% in mice with both high-fat diet and exercise training, while no change with those only with high-fat diet.”
From Effects of aerobic exercise on lipids and lipoproteins (2017) · cites this paper as [73]
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- Authors
- Rocco, D. D. F. M., Okuda, L. S., Pinto, R. S., Ferreira, F. D., Kubo, S. K., Nakandakare, E. R., Quintão, E. C. R., Catanozi, S., & Passarelli, M.
- Affiliations
- Universidade de São Paulo (BR)
- Corresponding author
- Passarelli, M.
- Venue
- Lipids
- Publisher
- Wiley
- Published
- 2011-04-09
- Volume
- 46
- Issue
- 7
- Pages
- 617-625
- ISSN
- 0024-4201 (ISSN-L)
- 1558-9307
- OpenAlex
- W2039523311
- Cited by
- 33
- References
- 46
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 5 · 2021: 3 · 2019: 2 · 2018: 3 · 2017: 5 · 2016: 2 · 2015: 5 · 2013: 3 · 2012: 1
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Lipids (publishedVersion)
- PubMed (publishedVersion)
- Scientific Electronic Library Online (São Paulo Research Foundation, Latin American and Caribbean Center on Health Sciences Information, Conselho Nacional de Desenvolvimento Científico e Tecnológico) (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Keywords
- aerobic exercise training, reverse cholesterol transport, cholesteryl ester transfer protein, CETP transgenic mice, HDL cholesterol, macrophages, LDL receptor, ABCA1, scavenger receptor class B type I, CYP7A1, atherosclerosis prevention, plasma lipids, liver cholesterol metabolism
- MeSH terms
- Animals, Biological Transport, Cholesterol, Cholesterol 7-alpha-Hydroxylase, Humans, Lipids, Lipoproteins, Lipoproteins, HDL, Lipoproteins, LDL, Liver, Macrophages, Male, Mice, Transgenic, Physical Conditioning, Animal, Receptors, LDL, Apolipoprotein A-I, ATP-Binding Cassette Transporters, CD36 Antigens, Atherosclerosis, Mice, Cholesterol Ester Transfer Proteins, ATP Binding Cassette Transporter 1
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