Different degrees of moderate iron deficiency modulate lipid metabolism of rats
Abstract
Severe iron deficiency affects lipid metabolism. To investigate whether moderate iron depletion also alters lipid variables-including lipid levels in serum and liver, hepatic lipogenesis, and fatty acid composition indicative of an impaired desaturation-we carried out experiments with rats fed 9, 13, and 18 mg iron/kg diet over a total of 5 wk. The study also included three pair-fed control groups and an ad libitum control group, fed with 50 mg iron/kg diet. The iron-depleted rats were classified as iron-deficient on the basis of reduced serum iron, hemoglobin concentration, and hematocrit. All moderately iron-deficient rats had significantly lower cholesterol concentrations in liver and serum lipoproteins than their pair-fed controls. Rats with the lowest dietary iron supply had higher concentrations of hepatic phosphatidylcholine (PC) and phosphatidylethanolamine (PE), lower activities of glucose-6-phosphate dehydrogenase, malic enzyme and fatty acid synthase, and higher triacylglycerol concentrations in serum lipoproteins than the corresponding pair-fed control rats. Moderate iron deficiency also depressed the serum phospholipid level. Moreover, several consistent significant differences in fatty acid composition of hepatic PC and PE occurred within moderate iron deficiency, which indicate impaired desaturation by delta-9 and delta-6 desaturases of saturated and essential fatty acids. We conclude that lipid variables, including cholesterol in liver and serum lipoproteins as well as fatty acid desaturation, reflect the gradations of iron status best and can be used as an indicator of the degree of moderate iron deficiency.
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Iron status has previously been suggested to affect lipid metabolism in both rats and humans.
“In keeping with this, iron status has previously been suggested to affect lipid metabolism in both rats and humans [40,41].”
From Associations of genetically determined iron status across the phenome: A mendelian randomization study (2019) · cites this paper as [40]
Cites
Guide for the Care and Use of Laboratory Animals: Eighth Edition
(2011) · doi:10.17226/12910 · Cited by 16,199 · see citing works
Reeves, P. G., Nielsen, F. H., & Fahey, G. C., Jr. (1993) · Journal of Nutrition · doi:10.1093/jn/123.11.1939 · Cited by 8,730 · see citing works
Preparation of fatty acid methyl esters and dimethylacetals from lipids with boron fluoride–methanol
Morrison, W. R., & Smith, L. M. (1964) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)40190-7 · Cited by 6,005 · see citing works
(1966) · Food and Cosmetics Toxicology · doi:10.1016/s0015-6264(66)80573-4 · Cited by 4,287 · see citing works
Lipid extraction of tissues with a low-toxicity solvent
Hara, A., & Radin, N. S. (1978) · Analytical Biochemistry · doi:10.1016/0003-2697(78)90046-5 · Cited by 2,566 · see citing works
Glucose-6-phosphate Dehydrogenase
Löhr, G. W., & Waller, H. D. (1974) · Elsevier eBooks · doi:10.1016/b978-0-12-091302-2.50026-8 · Cited by 1,189 · see citing works
[42] Glucose-6-phosphate dehydrogenase 6-phosphogluconic dehydrogenase
Kornberg, A., Horecker, B. L., Horecker, B. L., & Smyrniotis, P. Z. (1955) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(55)01046-x · Cited by 601 · see citing works
Purification and Properties of Rat Liver Microsomal Stearyl Coenzyme A Desaturase
Strittmatter, P., Spatz, L., Corcoran, D., Rogers, M. J., Setlow, B., & Redline, R. (1974) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.71.11.4565 · Cited by 469 · see citing works
Terpstra, A. H. M., Woodward, C. J. H., & Sánchez-Muniz, F. J. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90243-8 · Cited by 372 · see citing works
Hsu, R. Y., & Lardy, H. A. (1969) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(69)13042-6 · Cited by 226 · see citing works
An enzymatic assay for determining free and total cholesterol in tissue.
Hoff, J. L. D., Davidson, L. M., & Kritchevsky, D. (1978) · Clinical Chemistry · doi:10.1093/clinchem/24.3.433 · Cited by 136 · see citing works
Purification and partial characterization of linoleoyl-CoA desaturase from rat liver microsomes
岡安, 多., Nagao, M., Ishibashi, T., & Imai, Y. (1981) · Archives of Biochemistry and Biophysics · doi:10.1016/0003-9861(81)90061-8 · Cited by 135 · see citing works
[27] ATP citrate lyase (citrate-cleavage enzyme)
Takeda, Y., Suzuki, F., & Inoue, H. (1969) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(69)13032-3 · Cited by 124 · see citing works
Goodman, J. R., Warshaw, J. B., & Dallman, P. R. (1970) · Pediatric Research · doi:10.1203/00006450-197005000-00003 · Cited by 117 · see citing works
THE EFFECTS OF IRON DEFICIENCY ON THE HEPATOCYTE: A BIOCHEMICAL AND ULTRASTRUCTURAL STUDY
Dallman, P. R., & Goodman, J. R. (1971) · The Journal of Cell Biology · doi:10.1083/jcb.48.1.79 · Cited by 90 · see citing works
Iron Intake Influences Essential Fatty Acid and Lipid Composition of Rat Plasma and Erythrocytes
Cunnane, S. C., & McAdoo, K. R. (1987) · Journal of Nutrition · doi:10.1093/jn/117.9.1514 · Cited by 69 · see citing works
Sherman, A. R., Guthrie, H. A., Wolinsky, I., et al. (1978) · Journal of Nutrition · doi:10.1093/jn/108.1.152 · Cited by 67 · see citing works
[9] Fatty acid synthase from human liver
Roncari, D. A. K. (1981) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(81)71011-5 · Cited by 51 · see citing works
Iron Deficiency Lipemia in the Rat and Chick
Amine, E. K., & Hegsted, D. M. (1971) · Journal of Nutrition · doi:10.1093/jn/101.11.1575 · Cited by 43 · see citing works
Eder, K., Reichlmayr-Lais, A. M., & Kirchgeßner, M. (1991) · Journal of Chromatography A · doi:10.1016/0021-9673(91)85032-b · Cited by 43 · see citing works
Carnitine Levels in Iron-Deficient Rat Pups
Bartholmey, S. J., & Sherman, A. R. (1985) · Journal of Nutrition · doi:10.1093/jn/115.1.138 · Cited by 41 · see citing works
Hyperlipidemia in Offspring of Iron-deficient Rats
Guthrie, H. A., Froozani, M., Sherman, A. R., et al. (1974) · Journal of Nutrition · doi:10.1093/jn/104.10.1273 · Cited by 38 · see citing works
Eder, K., Reichlmayr-Lais, A. M., & Kirchgeßner, M. (1992) · Journal of Chromatography A · doi:10.1016/0021-9673(92)85111-6 · Cited by 38 · see citing works
Reduction of hepatic stearoyl‐CoA desaturase activity in rats fed iron‐deficient diets
Rao, G. A., Crane, R., & Larkin, E. C. (1983) · Lipids · doi:10.1007/bf02535401 · Cited by 36 · see citing works
Lipogenesis in iron‐deficient adult rats
Sherman, A. R. (1978) · Lipids · doi:10.1007/bf02533616 · Cited by 34 · see citing works
Effect of Dietary Fats on Lipogenesis in Iron Deficiency Anemic Chicks and Rats
Amine, E. K., Desilets, E. J., & Hegsted, D. M. (1976) · Journal of Nutrition · doi:10.1093/jn/106.3.405 · Cited by 32 · see citing works
Serum lipids in suckling and post‐weanling iron‐deficient rats
Sherman, A. R. (1979) · Lipids · doi:10.1007/bf02533501 · Cited by 29 · see citing works
Reduction of essential fatty acid deficiency in rats fed a low iron fat free diet
Rao, G. A., Manix, M., & Larkin, E. C. (1980) · Lipids · doi:10.1007/bf02534119 · Cited by 28 · see citing works
Fatty acid pattern of tissue phospholipids in copper and iron deficiencies
Johnson, S. B., Kramer, T. R., Briske‐Anderson, M., et al. (1989) · Lipids · doi:10.1007/bf02535252 · Cited by 25 · see citing works
Stangl, G. I., & Kirchgessner, M. (1998) · Zeitschrift für Ernährungswissenschaft · doi:10.1007/s003940050025 · Cited by 25 · see citing works
Self-citationLipemia in rodent iron-deficiency anemia.
Lewis, M., & Iammarino, R. M. (1971) · PubMed · Cited by 23 · see citing works
Eder, K., & Kirchgeßner, M. (1996) · The Journal of Nutritional Biochemistry · doi:10.1016/0955-2863(96)00008-3 · Cited by 23 · see citing works
Reduced plasma lecithin cholesterol acyl transferase activity in rats fed iron‐deficient diets
Rao, G. A., Crane, R. T., & Larkin, E. C. (1983) · Lipids · doi:10.1007/bf02534575 · Cited by 18 · see citing works
Reduced plasma lecithin cholesterol acyl transferase activity in rats fed iron‐deficient diets
Rao, G. A., Crane, R., & Larkin, E. C. (1983) · Lipids · doi:10.1007/bf02534532 · Cited by 17 · see citing works
Larkin, E. C., Jarratt, B. A., & Rao, G. A. (1986) · Nutrition Research · doi:10.1016/s0271-5317(86)80134-8 · Cited by 16 · see citing works
Sequential microultracentrifugation of lipoproteins in 100 microliters of serum.
Kupke, I. R., & Wörz-Zeugner, S. (1990) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)38777-0 · Cited by 16 · see citing works
HIROSUE, T., & Hosogai, Y. (1993) · Food and Chemical Toxicology · doi:10.1016/0278-6915(93)90048-4 · Cited by 8 · see citing works
Bio-Inorganic Regulation of Pathways of Carbohydrate and Lipid Metabolism
Sochor, M., Baquer, N. Z., & McLean, P. (2017) · Enzyme · doi:10.1159/000459043 · Cited by 8 · see citing works
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Simcox, J. A., & McClain, D. A. (2013) · Cell Metabolism · doi:10.1016/j.cmet.2013.02.007 · Cited by 545 · see citing works
Kwik-Uribe, C. L., Gietzen, D., German, J. B., Golub, M. S., & Keen, C. L. (2000) · The Journal of Nutrition · doi:10.1093/jn/130.11.2821 · Cited by 156 · see citing works
Interactions between hepatic iron and lipid metabolism with possible relevance to steatohepatitis
Ahmed, U. (2012) · World Journal of Gastroenterology · doi:10.3748/wjg.v18.i34.4651 · Cited by 121 · see citing works
Why cells need iron: a compendium of iron utilisation
Teh, M. R., Armitage, A. E., & Drakesmith, H. (2024) · Trends in Endocrinology & Metabolism · doi:10.1016/j.tem.2024.04.015 · Cited by 114 · see citing works
Gill, D., Benyamin, B., Moore, L. S. P., Monori, G., Zhou, A., Koskeridis, F., Evangelou, E., Laffan, M., Walker, A. P., Tsilidis, K. K., et al. (2019) · PLOS Medicine · doi:10.1371/journal.pmed.1002833 · Cited by 63 · see citing works
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Badaracco, M. E., Siri, M. V. R., & Pasquini, J. M. (2010) · BioFactors · doi:10.1002/biof.90 · Cited by 63 · see citing works
Nash, insulin resistance and iron
MACHADO, M., & CORTEZ‐PINTO, H. (2006) · Liver International · doi:10.1111/j.1478-3231.2006.01394.x · Cited by 18 · see citing works
Pompano, L. M., & Haas, J. D. (2019) · The Journal of Nutrition · doi:10.1093/jn/nxy271 · Cited by 14 · see citing works
- Authors
- Stangl, G. I., & Kirchgessner, M.
- Affiliations
- Technical University of Munich (DE)
- Corresponding author
- Stangl, Gabriele I.
- ORCID
- Stangl, Gabriele I.
- Venue
- Lipids
- Publisher
- Wiley
- Published
- 1998-09
- Volume
- 33
- Issue
- 9
- Pages
- 889-895
- ISSN
- 0024-4201 (ISSN-L)
- 1558-9307
- OpenAlex
- W2025996961
- Cited by
- 84
- References
- 38
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 1 · 2021: 5 · 2020: 3 · 2019: 4 · 2018: 5 · 2017: 4 · 2016: 3 · 2015: 6 · 2014: 7 · 2013: 3 · 2012: 7
- FWCI
- 1.59
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Lipids (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Nursing › Nutrition and Dietetics › Trace Elements in Health
- Keywords
- lipid metabolism, hepatic lipogenesis, fatty acid desaturation, delta-9 desaturase, delta-6 desaturase, serum cholesterol, hepatic phospholipids, phosphatidylcholine, phosphatidylethanolamine, triglycerides, fatty acid composition, dietary iron restriction
- MeSH terms
- Iron Deficiencies, Animals, Fatty Acids, Lipids, Lipoproteins, Liver, Male, Phospholipids, Weight Gain, Rats, Sprague-Dawley, Lipid Metabolism, Rats
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