Cited by 174Protein Hydrolysis and Bioactive PeptidesRenin-Angiotensin System StudiesPeptidase Inhibition and Analysis

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Kinetics of Enzyme Inhibition and Antihypertensive Effects of Hemp Seed ( Cannabis sativa L.) Protein Hydrolysates

Girgih, Abraham T., Udenigwe, Chibuike C., Li, Huan, Adebiyi, Abayomi P., Aluko, Rotimi E.·2011·Journal of the American Oil Chemists' Society

DOI 10.1007/s11746-011-1841-9

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Abstract

The aim of this study was to determine the antihypertensive effects of enzymatic hemp seed protein hydrolysate (HPH) and its peptide fractions. Hemp seed protein isolate was digested by the sequential action of pepsin and pancreatin to mimic gastrointestinal digestion in human beings. The resultant HPH was separated by membrane ultrafiltration into peptide fractions with different sizes (<1 and 1–3 kDa). The HPH led to significantly higher ( P < 0.05) in vitro inhibition of the activities of angiotensin I‐converting enzyme (ACE) and renin, the two main enzymes involved in abnormal blood pressure elevation (hypertension). Kinetic studies showed that HPH and peptide fractions inhibited renin and ACE activities in a mixed‐type pattern, indicating binding to areas other than the active site. Oral administration of HPH (200 mg/kg body weight) to spontaneously hypertensive rats led to significant reductions ( P < 0.05) in systolic blood pressure (SBP) that reached a maximum of −30 mmHg after 8 h. In contrast, the hypotensive effects of peptide fractions (<1 and 1–3 kDa) had a maximum value of about −15 mmHg after 6–8 h post oral administration. The results suggest a synergistic antihypertensive effect of the peptides present within HPH; this effect was reduced significantly ( P < 0.05) upon separation into peptide fractions.

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Physicochemical and Functional Properties of Hemp ( Cannabis sativa L.) Protein Isolate
Tang, C.-H., Ten, Z., Wang, X.-S., & Yang, X.-Q. (2006) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0619176 · Cited by 405

Show all 0 references

Research works printed but not linked: 32

10.1016/j.peptides.2008.02.005 · DOI
Aluko RE, 2008, Antihypertensive properties of plant‐derived inhibitors of angiotensin I‐converting enzyme activity: a review, Recent Prog Med Plants, 22, 541
10.1016/S0140-6736(79)92186-X · DOI
10.1016/j.foodchem.2009.05.080 · DOI
10.1016/j.jnutbio.2007.11.010 · DOI · Paper page
Wang XS, 2009, Characterization and antioxidant properties of hemp protein hydrolysate obtained with neutrase, Food Technol Biotechnol, 47, 428
10.1016/j.foodchem.2007.06.064 · DOI
10.1021/jf102636b · DOI
10.1017/S0022029999003982 · DOI
10.1007/s11746-010-1686-7 · DOI
10.1093/jn/134.4.980S · DOI
10.1016/j.jff.2009.01.009 · DOI
10.1093/ndt/gfm363 · DOI
10.1021/jf102538g · DOI
10.1016/0003-2697(78)90586-9 · DOI
10.1021/jf60226a042 · DOI
10.1016/j.jnutbio.2005.05.007 · DOI
AukemaHM GauthierJ JiaY RoyM LiH AlukoRE(2011).Distinctive effects of plant protein sources on renal disease progression and associated cardiac hypertrophy in experimental kidney disease.Mol Nutr Food Res.doi: 10.1002/mnfr.201000558 · DOI
10.1016/j.foodchem.2008.10.050 · DOI
10.1016/j.foodchem.2007.07.030 · DOI
10.1016/j.foodres.2010.05.003 · DOI
10.1016/0308-8146(95)93934-J · DOI
10.1021/jf970294g · DOI
10.1159/000007679 · DOI
10.1007/s00217-007-0689-6 · DOI
10.3168/jds.S0022-0302(99)75258-6 · DOI
10.1016/S0032-9592(99)00098-9 · DOI
10.1021/jf051263l · DOI
10.3317/jraas.2003.008 · DOI
10.1021/jf0710635 · DOI
10.1016/j.foodchem.2009.04.086 · DOI
10.1016/j.foodchem.2008.03.059 · DOI

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Peptide Fractions from Pepsin-digested Moringa oleifera Seed Proteins Inhibit Hemoglobin Glycation and Carbohydrate-hydrolyzing Enzymes
Ekun, O. (2023) · Biology, Medicine, & Natural Product Chemistry · doi:10.14421/biomedich.2023.121.413-422 · Cited by 1
“It, therefore, follows that a high peptide yield is indicative of increased proteolysis and resultant peptide release (Girgih et al., 2011).”
BackgroundMethod
Hempseed (Cannabis sativa)
Syed, I., Garg, S., Agarwal, S., & Mohapatra, N. (2020) · Springer eBooks · doi:10.1007/978-981-15-4194-0_15 · Cited by 3
Valorization Potential of Oilseed Press Cakes from Pumpkin (Cucurbita pepo) and Hemp (Cannabis sativa)
Dyankova, S. M., Solak, A., Митева, Д., Doneva, M., Metodieva, P., & Nacheva, I. B. (2026) · AppliedChem · doi:10.3390/appliedchem6030057
Hydrolysates from aduwa (B.aegyptiaca Del): A new source of inhibitory peptide
Friday, O., Abraham, G., Ojotu, E., Jackson, V., & Oneh, A. (2025) · Dietary Supplements and Nutraceuticals · doi:10.31989/dsn.v4i12.1879
“Pancreatin: The modified method of [24] was used.”
Ribes nigrum and Juglans regia, Potential Functional Foods as a Source of Protein Tyrosine Phosphatase Enzyme: Biochemical and Kinetics Studies
Selvaraj, K., Chand, S., Katare, D., & Chaudhary, N. (2022) · Biointerface Research in Applied Chemistry · doi:10.33263/briac131.061
“Ki value provides information that assists in understanding the ability of the inhibitor to effectively block or reduce enzyme activity and the quantity of inhibitor needed either for the reaction to proceed or for the enzyme activities to be inhibited [64-66].”
Background
Effects of exopeptidase treatment on angiotensin I-converting enzyme inhibitory activity, antihypertensive activity and taste of whey protein hydrolysates
Cheung, L. K. (2014) · doi:10.14288/1.0167402
“reduction in SHRs lasted for at least 24 h (Girgih et al., 2011; Ruiz-Giménez et al., 2012).”
Background

Authors: Girgih, A. T., Udenigwe, C. C., Li, H., Adebiyi, A. P., & Aluko, R. E.
Affiliations: University of Manitoba (CA)
Corresponding author: Aluko, Rotimi E.
ORCID: Girgih, Abraham T.
Udenigwe, Chibuike C.
Li, Huan
Aluko, Rotimi E.
Venue: Journal of the American Oil Chemists' Society
Publisher: Wiley
Published: 2011-05-10
Volume: 88
Issue: 11
Pages: 1767-1774
ISSN: 0003-021X (ISSN-L)
1558-9331
DOI: 10.1007/s11746-011-1841-9
OpenAlex: W2093049132
Cited by: 174
References: 0
Citations by year: 2026: 5 · 2025: 9 · 2024: 12 · 2023: 7 · 2022: 12 · 2021: 15 · 2020: 15 · 2019: 12 · 2018: 11 · 2017: 17 · 2016: 17 · 2015: 11 · 2014: 9 · 2013: 16 · 2012: 4
FWCI: 4.02
Type: journal article
Language: en
Open access: closed
Licence: onlinelibrary.wiley.com/termsAndConditions#vor (vor)
Locations: Journal of the American Oil Chemists Society (publishedVersion)
Funders: Natural Sciences and Engineering Research Council of Canada
Research Executive Agency
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Protein Hydrolysis and Bioactive Peptides
Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Renin-Angiotensin System Studies
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Peptidase Inhibition and Analysis
Keywords: Cannabis sativa, antihypertensive peptides, angiotensin-converting enzyme inhibition, renin inhibition, gastrointestinal digestion, spontaneously hypertensive rats, blood pressure reduction, synergistic effect

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Kinetics of Enzyme Inhibition and Antihypertensive Effects of Hemp Seed ( Cannabis sativa L.) Protein Hydrolysates

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