Cited by 87Antifungal resistance and susceptibilityYeasts and Rust Fungi StudiesBacterial Identification and Susceptibility Testing

Candida auris: diagnostic challenges and emerging opportunities for the clinical microbiology laboratory.

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Keighley, C., Garnham, K., Harch, S. A. J., Robertson, M., Chaw, K., Teng, J. C., Chen, S. C.-A.·2021·Current Fungal Infection Reports

Abstract

Purpose of review This review summarises the epidemiology of Candida auris infection and describes contemporary and emerging diagnostic methods for detection and identification of C. auris. Recent findings A fifth C. auris clade has been described. Diagnostic accuracy has improved with development of selective/differential media for C. auris. Advances in spectral databases of matrix-associated laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) systems have reduced misidentification. Direct detection of C. auris in clinical specimens using real time PCR is increasingly used, as is whole genome sequencing (WGS) to track nosocomial spread and to study phylogenetic relationships and drug resistance. Summary C. auris is an important transmissible, nosocomial pathogen. The microbiological laboratory diagnostic capacity has extended beyond culture-based methods to include PCR and WGS. Microbiological techniques on the horizon include the use of MALDI-TOF MS for early echinocandin antifungal susceptibility testing (AST) and expansion of the versatile and information-rich WGS methods for outbreak investigation.

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  • Clinical laboratories might not routinely distinguish C. auris from other Candida species for nonsterile specimen types, so StC events could have been missed.

    “StC events could have been missed because of missed screening opportunities, the insensitivity of culture (9), treating clinical laboratories that might not routinely distinguish C. auris from other Candida species for nonsterile specimen types (10), and the fact that US C. auris data from 2024 are not finalized, meaning some patients might not have had sufficient lead time for clinical cases to”

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Authors
Keighley, C., Garnham, K., Harch, S. A. J., Robertson, M., Chaw, K., Teng, J. C., & Chen, S. C.-A.
Affiliations
  • The University of Sydney (AU)
  • Royal North Shore Hospital (AU)
  • South Australia Pathology (AU)
  • Gosford Hospital (AU)
  • NSW Health Pathology (AU)
Corresponding author
Keighley, C.
Venue
Current Fungal Infection Reports
Published
2021-06-23
Volume
15
Issue
3
Pages
116-126
ISSN
  • 1936-3761 (ISSN-L)
  • 1936-377X
OpenAlex
W3173974021
Cited by
87
References
6
Citations by year
2026: 22 · 2025: 15 · 2024: 20 · 2023: 17 · 2022: 10 · 2021: 3
FWCI
1.46
Type
journal article
Language
en
Open access
Locations
  • Current Fungal Infection Reports (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
Keywords
Candida auris, fungal infection diagnosis, MALDI-TOF mass spectrometry, real-time PCR, whole genome sequencing, nosocomial transmission, outbreak investigation, antifungal resistance

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