Effect of elicitation and precursor feeding on accumulation of 20-hydroxyecdysone in Achyranthes aspera Linn. cell suspension cultures
Open accessAbstract
20-Hydroxyecdysone is one of the most common ecdysteroids in plants with potential therapeutic applications. In this study, cell suspension cultures of Achyranthes aspera were raised in shake flasks to investigate the production of 20-hydroxyecdysone. The quantification and characterization of 20-hydroxyecdysone in the cultures were done by High performance liquid chromatography (HPLC) and Liquid Chromatography-quadrupole time-of- flight mass spectrometry (LC-Q-TOF) analyses. For raising the suspension, calli initiated from in vitro grown leaf explants were cultured in liquid Murashige and Skoog (MS) medium augmented with combinations of 2, 4-dichlorophenoxyacetic acid (1 mg L-1) and α-naphthaleneacetic acid (1 mg L-1). Maximum growth index of the cell suspension was 9.9, which was achieved during 20th day of culture (final phase of exponential growth). At this stage, the biomass accumulated was 1.09 ± 0.09 g dry weight (DW) and the 20-hydroxyecdysone concentration was 0.24 mg g-1 DW. Eliciting the cultures with 0.6 mM Methyl jasmonate for 6 days; enhanced the production of 20-hydroxyecdysone production to 0.35 mg g-1 DW. By augmenting the cultures with the precursors namely cholesterol (10 mg L-1) and 7-dehydrocholesterol (10 mg L-1), production of 20-hydroxyecdysone was boosted to 0.31 mg g-1 DW and 0.28 mg g-1 DW respectively.
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Callus cultures also give low ecdysteroid production.
“The same applies to callus cultures”
From Ecdysteroids (2021) · cites this paper as [173]
In vitro cultures from ecdysteroid-rich plants give disappointing results because production is generally very low, even after precursor feeding or hormonal elicitation.
“In vitro cultures from ecdysteroid-rich plants have been developed, but they give disappointing results as the production has been generally very low, even after precursor feeding or hormonal elicitation [172][173][174].”
From Ecdysteroids (2021) · cites this paper as [173]
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- Authors
- John, R., Shajitha, P. P., Devassy, A., & Mathew, L.
- Affiliations
- Mahatma Gandhi University (IN)
- Corresponding author
- Mathew, Linu
- ORCID
- Mathew, Linu
- Venue
- Physiology and Molecular Biology of Plants
- Published
- 2018-01-27
- Volume
- 24
- Issue
- 2
- Pages
- 275-284
- ISSN
- 0971-5894 (ISSN-L)
- 0974-0430
- PMC
- PMC5834991
- OpenAlex
- W2786439607
- Cited by
- 16
- References
- 41
- Citations by year
- 2025: 3 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 2 · 2018: 1
- FWCI
- 0.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Physiology and Molecular Biology of Plants (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- University Grants Commission, New Delhi (F. No- 41-552/2012 SR)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Phytochemical Studies and Bioactivities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- 20-hydroxyecdysone, Achyranthes aspera, ecdysteroids, cell suspension culture, elicitation, methyl jasmonate, precursor feeding, cholesterol, 7-dehydrocholesterol, plant secondary metabolite production, callus induction, plant growth regulators
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