Cited by 23Plant Stress Responses and TolerancePlant tissue culture and regenerationPlant biochemistry and biosynthesis

Salinity-induced modifications on growth, physiology and 20-hydroxyecdysone levels in Brazilian-ginseng [Pfaffia glomerata (Spreng.) Pedersen]

Felipe, Sérgio Heitor Sousa, Batista, Diego Silva, Vital, Camilo Elber, Chagas, Kristhiano, Silva, Priscila Oliveira, Silva, Tatiane Dulcineia, Fortini, Evandro Alexandre, Correia, Ludmila Nayara de Freitas +5 more·2019·Plant Physiology and Biochemistry

Abstract

- Salinity is a major threat to agriculture. However, depending on the concentration of soluble salts in soil, increased secondary metabolite levels can occur with no major damages to plant growth and development. The phytoecdysteroid (PE) 20-hydroxyecdysone (20E) is a secondary metabolite with biotechnological, medicinal, pharmaceutical and agrochemical applicability. Here, we characterize the responses (growth and physiology) of Pfaffia glomerata under different NaCl concentrations and examine the production of 20E as affected by salinity. Forty-day-old plants grown in greenhouse were exposed to 0, 120, 240, 360 or 480 mM of NaCl for 11 days. Moderate salinity (i.e., 120 mM of NaCl) led to increased 20E concentrations in leaves (47%) relative to the control with no significant effect on photosynthesis and biomass accumulation, thus allowing improved 20E contents on a per whole-plant basis. In contrast, plants under high salinity (i.e., 240-480 mM of NaCl) displayed similar 20E concentrations in leaves compared to the control, but with marked impairments to biomass accumulation and photosynthetic performance (coupled with decreased sucrose and starch levels) in parallel to nutritional imbalance. High salinity also strongly increased salicylic acid levels, antioxidant enzyme activities, and osmoregulatory status. Regardless of stress severity, 20E production was accompanied by the upregulation of Spook and Phantom genes. Our findings suggest that P. glomerata cultivation in moderate salinity soils can be considered as a suitable agricultural option to increase 20E levels, since metabolic and structural complexity that makes its artificial synthesis very difficult.

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Crossref · 10.1016/j.plaphy.2019.05.002

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Authors
Felipe, S. H. S., Batista, D. S., Vital, C. E., Chagas, K., Silva, P. O., Silva, T. D., Fortini, E. A., Correia, L. N. D. F., Ávila, R. T., Maldaner, J., Festucci-Buselli, R. A., DaMatta, F. M., & Otoni, W. C.
Affiliations
  • Universidade Federal de Viçosa (BR)
  • Universidade Estadual do Maranhão (BR)
  • Universidade Federal do Amazonas (BR)
  • Universidade Federal Rural da Amazônia (BR)
Corresponding author
Otoni, Wagner Campos
Venue
Plant Physiology and Biochemistry
Publisher
Elsevier BV
Published
2019-07
Volume
140
Pages
43-54
ISSN
0981-9428 (ISSN-L)
OpenAlex
W2942861535
Cited by
23
References
81
Citations by year
2026: 4 · 2025: 4 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 1
FWCI
1.84
Type
journal article
Language
en
Open access
closed
Locations
  • Plant Physiology and Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
  • Fundação de Amparo à Pesquisa do Estado de Minas Gerais
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
  • SHSF was recipient of a scholarship from CAPES
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
Keywords
Pfaffia glomerata, salt stress, sodium chloride, 20-hydroxyecdysone, phytoecdysteroids, secondary metabolites, photosynthesis, biomass accumulation, salicylic acid, antioxidant enzymes, osmoregulation, medicinal plant cultivation
MeSH terms
Ecdysterone, Panax, Photosynthesis, Sodium Chloride, Biomass, Salinity

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