Salinity-induced modifications on growth, physiology and 20-hydroxyecdysone levels in Brazilian-ginseng [Pfaffia glomerata (Spreng.) Pedersen]
Abstract
- Salinity is a major threat to agriculture. However, depending on the concentration of soluble salts in soil, increased secondary metabolite levels can occur with no major damages to plant growth and development. The phytoecdysteroid (PE) 20-hydroxyecdysone (20E) is a secondary metabolite with biotechnological, medicinal, pharmaceutical and agrochemical applicability. Here, we characterize the responses (growth and physiology) of Pfaffia glomerata under different NaCl concentrations and examine the production of 20E as affected by salinity. Forty-day-old plants grown in greenhouse were exposed to 0, 120, 240, 360 or 480 mM of NaCl for 11 days. Moderate salinity (i.e., 120 mM of NaCl) led to increased 20E concentrations in leaves (47%) relative to the control with no significant effect on photosynthesis and biomass accumulation, thus allowing improved 20E contents on a per whole-plant basis. In contrast, plants under high salinity (i.e., 240-480 mM of NaCl) displayed similar 20E concentrations in leaves compared to the control, but with marked impairments to biomass accumulation and photosynthetic performance (coupled with decreased sucrose and starch levels) in parallel to nutritional imbalance. High salinity also strongly increased salicylic acid levels, antioxidant enzyme activities, and osmoregulatory status. Regardless of stress severity, 20E production was accompanied by the upregulation of Spook and Phantom genes. Our findings suggest that P. glomerata cultivation in moderate salinity soils can be considered as a suitable agricultural option to increase 20E levels, since metabolic and structural complexity that makes its artificial synthesis very difficult.
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Pfaffia glomerata moderates salinity by increasing the production of 20-HE, salicylic acid, osmolytes, and antioxidants.
“However, Pfaffia glomerata moderates salinity by increasing the production of 20-HE, salicylic acid, osmolytes, and antioxidants.”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [198]
Salinity upregulates several PE biosynthesis genes and other genes such as Spook and Phantom, enhancing PE production.
“Additionally, salinity upregulates several PE biosynthesis genes and other genes such as Spook and Phantom, enhancing PE production [198].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [198]
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da Silva, T. F. O., Yamaguchi, C. S., Ribeiro, S. T. C., Avíncola, A. D. S., Pilau, E. J., Porto, C., de Oliveira, A. J. B., & Gonçalves, R. A. C. (2021) · Phytochemistry Letters · doi:10.1016/j.phytol.2021.02.009 · Cited by 6 · see citing works
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Self-citationPhysiological and Proteomic Shifts in Pfaffia glomerata Under Drought and Elevated CO₂
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Self-citation
- Authors
- Felipe, S. H. S., Batista, D. S., Vital, C. E., Chagas, K., Silva, P. O., Silva, T. D., Fortini, E. A., Correia, L. N. D. F., Ávila, R. T., Maldaner, J., Festucci-Buselli, R. A., DaMatta, F. M., & Otoni, W. C.
- Affiliations
- Universidade Federal de Viçosa (BR)
- Universidade Estadual do Maranhão (BR)
- Universidade Federal do Amazonas (BR)
- Universidade Federal Rural da Amazônia (BR)
- Corresponding author
- Otoni, Wagner Campos
- ORCID
- Venue
- Plant Physiology and Biochemistry
- Publisher
- Elsevier BV
- Published
- 2019-07
- Volume
- 140
- Pages
- 43-54
- ISSN
- 0981-9428 (ISSN-L)
- OpenAlex
- W2942861535
- Cited by
- 23
- References
- 81
- Citations by year
- 2026: 4 · 2025: 4 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 1
- FWCI
- 1.84
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Plant Physiology and Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- SHSF was recipient of a scholarship from CAPES
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- Pfaffia glomerata, salt stress, sodium chloride, 20-hydroxyecdysone, phytoecdysteroids, secondary metabolites, photosynthesis, biomass accumulation, salicylic acid, antioxidant enzymes, osmoregulation, medicinal plant cultivation
- MeSH terms
- Ecdysterone, Panax, Photosynthesis, Sodium Chloride, Biomass, Salinity
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