Cited by 57Probiotics and Fermented FoodsMycotoxins in Agriculture and FoodPlant-Microbe Interactions and Immunity

Isolation of lactic acid bacteria from grape fruit: Antifungal activities, probiotic properties, and in vitro detoxification of ochratoxin A

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Taroub, Bouzaiene, Salma, Lasram, Manel, Ziadi, Ouzari, Hadda-Imen, Hamdi, Zohra, Moktar, Hamdi·2019·Annals of Microbiology

Abstract

The aim of this study was to isolate lactic acid bacteria from two varieties of grape bunch cultivated in Tunisia (cardinal and red-globe) in order to assess their ability to inhibit the growth of their most widespread contaminant, Aspergillus niger aggrégats and Aspergillus carbonarius. Antifungal activity of 18 isolates was investigated using overlay technique; selected isolates were than identified using 16s rDNA sequence analysis. Isolates with antifungal activities were screened out and studied for their probiotic properties using in vitro tests (tolerance to simulated gastric jus, bile salts, hydrophobicity properties).The most efficient strain was also investigated for its ability to reduce the concentration of ochratoxin A (OTA) on liquid medium. Determination of OTA content in media was released using HPLC analysis. Selected strains (RG7B) (C11C) and (RG8A) showing a good antifungal activities against Aspergillus niger aggrégats and Aspergillus carbonarius were identified as Pediococcus pentosaceus and Lactobacillus plantarum, respectively. Pediococcus pentosaceus (RG7B) showed promising potential probiotic characteristics and had a high ability for OTA removal after 48 h of incubation in both MRS and PBS media. The OTA removal percentage was significantly higher in MRS than in PBS media (84 and 25%, respectively). This study provides evidence for the control of black Aspergillus growth in grape and OTA detoxifying by the use of autochthones LAB strains having antifungal effect and probiotic potential.

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Crossref · 10.1007/s13213-018-1359-6

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Authors
Taroub, B., Salma, L., Manel, Z., Ouzari, H.-I., Hamdi, Z., & Moktar, H.
Affiliations
Center of Biotechnogy of Borj Cédria (TN)
Venue
Annals of Microbiology
Published
2018-08-08
Volume
69
Issue
1
Pages
17-27
ISSN
  • 0003-4649 (ISSN-L)
  • 1590-4261
  • 1869-2044
OpenAlex
W2885570827
Cited by
57
References
68
Citations by year
2026: 4 · 2025: 8 · 2024: 3 · 2023: 8 · 2022: 16 · 2021: 10 · 2020: 4 · 2019: 3
FWCI
4.00
Type
journal article
Language
en
Open access
Locations
Annals of Microbiology (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant-Microbe Interactions and Immunity
Keywords
lactic acid bacteria, grapes, antifungal activity, probiotic properties, Pediococcus pentosaceus, Lactobacillus plantarum, Aspergillus niger, Aspergillus carbonarius, black rot, autochthonous strains, bile salt tolerance, hydrophobicity

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