Cited by 654Probiotics and Fermented FoodsEssential Oils and Antimicrobial ActivityMicrobial Natural Products and Biosynthesis

Purification and Characterization of Novel Antifungal Compounds from the Sourdough Lactobacillus plantarum Strain 21B

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Lavermicocca, Paola, Valerio, Francesca, Evidente, Antonio, Lazzaroni, Silvia, Corsetti, Aldo, Gobbetti, Marco·2000·Applied and Environmental Microbiology

Abstract

Sourdough lactic acid bacteria were selected for antifungal activity by a conidial germination assay. The 10-fold-concentrated culture filtrate of Lactobacillus plantarum 21B grown in wheat flour hydrolysate almost completely inhibited Eurotium repens IBT18000, Eurotium rubrum FTDC3228, Penicillium corylophilum IBT6978, Penicillium roqueforti IBT18687, Penicillium expansum IDM/FS2, Endomyces fibuliger IBT605 and IDM3812, Aspergillus niger FTDC3227 and IDM1, Aspergillus flavus FTDC3226, Monilia sitophila IDM/FS5, and Fusarium graminearum IDM623. The nonconcentrated culture filtrate of L. plantarum 21B grown in whole wheat flour hydrolysate had similar inhibitory activity. The activity was fungicidal. Calcium propionate at 3 mg ml(-1) was not effective under the same assay conditions, while sodium benzoate caused inhibition similar to L. plantarum 21B. After extraction with ethyl acetate, preparative silica gel thin-layer chromatography, and chromatographic and spectroscopic analyses, novel antifungal compounds such as phenyllactic and 4-hydroxy-phenyllactic acids were identified in the culture filtrate of L. plantarum 21B. Phenyllactic acid was contained at the highest concentration in the bacterial culture filtrate and had the highest activity. It inhibited all the fungi tested at a concentration of 50 mg ml(-1) except for P. roqueforti IBT18687 and P. corylophilum IBT6978 (inhibitory concentration, 166 mg ml(-1)). L. plantarum 20B, which showed high antimold activity, was also selected. Preliminary studies showed that phenyllactic and 4-hydroxy-phenyllactic acids were also contained in the bacterial culture filtrate of strain 20B. Growth of A. niger FTDC3227 occurred after 2 days in breads started with Saccharomyces cerevisiae 141 alone or with S. cerevisiae and Lactobacillus brevis 1D, an unselected but acidifying lactic acid bacterium, while the onset of fungal growth was delayed for 7 days in bread started with S. cerevisiae and selected L. plantarum 21B.

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Crossref · 10.1128/aem.66.9.4084-4090.2000

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  • In addition to major economic losses associated with spoilage, some fungi pose a threat to food safety due to their ability to produce mycotoxins.

    “In addition to the major economic losses associated with spoilage, some fungi pose a threat to food safety due to their ability to produce mycotoxins [139,148,151,152,153,154,155].”

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Authors
Lavermicocca, P., Valerio, F., Evidente, A., Lazzaroni, S., Corsetti, A., & Gobbetti, M.
Affiliations
  • National Research Council (IT)
  • University of Naples Federico II (IT)
  • University of Perugia (IT)
  • University of Bari Aldo Moro (IT)
Venue
Applied and Environmental Microbiology
Published
2000-09
Volume
66
Issue
9
Pages
4084-4090
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2140130533
Cited by
654
References
25
Citations by year
2026: 14 · 2025: 13 · 2024: 17 · 2023: 35 · 2022: 41 · 2021: 35 · 2020: 36 · 2019: 30 · 2018: 39 · 2017: 30 · 2016: 26 · 2015: 35 · 2014: 41 · 2013: 41 · 2012: 36
FWCI
12.75
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
Keywords
Lactobacillus plantarum, sourdough, antifungal agents, phenyllactic acid, lactic acid bacteria, fungal growth inhibition, conidial germination, Aspergillus niger, Penicillium, Fusarium graminearum, culture filtrate
MeSH terms
Antifungal Agents, Bread, Fungi, Lactates, Lactobacillus, Gas Chromatography-Mass Spectrometry, Microbial Sensitivity Tests, Phenylpropionates, Spores, Fungal

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