Isolation and sequencing of a complementary deoxyribonucleic acid clone encoding human placental 173-estradiol dehydrogenase: Identification of the putative cofactor binding site
Abstract
17 beta-Estradiol dehydrogenase (EC 1.1.1.62) catalyzes the interconversion of estradiol and estrone in human term placenta. We have raised a specific polyclonal antibody to this abundant placental enzyme to study its role in late pregnancy events and its molecular biologic characteristics. In this work the 17 beta-estradiol dehydrogenase antibody was used to isolate and sequence a complementary deoxyribonucleic acid clone encoding about 98% of the amino acid sequence of the 17 beta-estradiol dehydrogenase molecule. This sequence verifies previous sequence data on the molecule's steroid binding site and also localizes a putative nicotinamide adenine dinucleotide binding region similar to that of many other pyridine nucleotide-dependent dehydrogenases. Isolation of the complementary deoxyribonucleic acid for 17 beta-estradiol dehydrogenase expands our knowledge of the structure-function relationships of the enzyme and is a major step in our understanding of its biologic function in pregnancy.
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In the late 1980s, three independent groups reported the cloning of its cDNA, the first of any human HSD.
“In the late 1980s, three independent groups reported the cloning of its cDNA, the first of any human HSD (503-505).”
From The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders (2011) · cites this paper as [504]
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The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders
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- Authors
- Gast, M. J., Sims, H. F., Murdock, G. L., Gast, P. M., & Strauss, A. W.
- Affiliations
- Washington University in St. Louis (US)
- Corresponding author
- Gast, Peter M.
- ORCID
- Strauss, Arnold W.
- Venue
- American Journal of Obstetrics and Gynecology
- Publisher
- Elsevier BV
- Published
- 1989-12
- Volume
- 161
- Issue
- 6
- Pages
- 1726-1731
- ISSN
- 0002-9378 (ISSN-L)
- OpenAlex
- W2029595735
- Cited by
- 45
- References
- 18
- Citations by year
- 2018: 2
- FWCI
- 1.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- American Journal of Obstetrics and Gynecology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Health Sciences › Medicine › Obstetrics and Gynecology › Pregnancy and preeclampsia studies
- Keywords
- human placenta, cDNA cloning, polyclonal antibodies, NAD+ binding site, cofactor binding site, steroid binding sites, structure-function relationship, late pregnancy
- MeSH terms
- Amino Acid Sequence, Base Sequence, Binding Sites, Cloning, Molecular, DNA, Estradiol Dehydrogenases, Female, Humans, Molecular Sequence Data, NAD, Placenta, Pregnancy, 17-Hydroxysteroid Dehydrogenases
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