Cited by 302Steroid Chemistry and BiochemistryEstrogen and related hormone effectsAdrenal Hormones and Disorders

Complete amino acid sequence of human placental 17β-hydroxysteroid dehydrogenase deduced from cDNA

Peltoketo, H., Isomaa, V., Mäentausta, O., Vihko, R.·1988·FEBS Letters

Abstract

cDNA clones for 17 beta-hydroxysteroid dehydrogenase (17-HSD; EC 1.1.1.62) were isolated from a placental lambda gt11 expression library using polyclonal antibodies against placental 17-HSD. The largest cDNA contained 1325 nucleotides, consisting of a short 5'-noncoding segment, a coding segment of 987 nucleotides terminated by a TAA codon, and a 329 nucleotide long 3'-noncoding segment. The open reading frame encoded a polypeptide of 327 amino acid residues with a predicted Mr of 34853. The amino acid sequence of 23 N-terminal amino acids determined from purified 17-HSD agreed with the sequence deduced from cDNA. The deduced amino acid sequence also contained two peptides previously characterized from the proposed catalytic area of placental 17-HSD.

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Crossref · 10.1016/0014-5793(88)80548-9

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Authors
Peltoketo, H., Isomaa, V., Mäentausta, O., & Vihko, R.
Affiliations
University of Oulu (FI)
Corresponding author
Peltoketo, H.
Venue
FEBS Letters
Publisher
Wiley
Published
1988-10-24
Volume
239
Issue
1
Pages
73-77
ISSN
  • 0014-5793 (ISSN-L)
  • 1873-3468
OpenAlex
W2073005394
Cited by
294
References
26
Citations by year
2025: 2 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 4 · 2013: 1 · 2012: 3
FWCI
4.22
Type
journal article
Language
en
Open access
closed
Locations
  • FEBS Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
Keywords
17β-hydroxysteroid dehydrogenase, placenta, cDNA cloning, amino acid sequence, open reading frames, N-terminal sequence, steroid metabolism
MeSH terms
Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Female, Humans, Molecular Sequence Data, Placenta, Pregnancy, Protein Conformation, 17-Hydroxysteroid Dehydrogenases, Restriction Mapping

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