Complete amino acid sequence of human placental 17β-hydroxysteroid dehydrogenase deduced from cDNA
Abstract
cDNA clones for 17 beta-hydroxysteroid dehydrogenase (17-HSD; EC 1.1.1.62) were isolated from a placental lambda gt11 expression library using polyclonal antibodies against placental 17-HSD. The largest cDNA contained 1325 nucleotides, consisting of a short 5'-noncoding segment, a coding segment of 987 nucleotides terminated by a TAA codon, and a 329 nucleotide long 3'-noncoding segment. The open reading frame encoded a polypeptide of 327 amino acid residues with a predicted Mr of 34853. The amino acid sequence of 23 N-terminal amino acids determined from purified 17-HSD agreed with the sequence deduced from cDNA. The deduced amino acid sequence also contained two peptides previously characterized from the proposed catalytic area of placental 17-HSD.
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In the late 1980s, three independent groups reported the cloning of its cDNA, the first of any human HSD.
“In the late 1980s, three independent groups reported the cloning of its cDNA, the first of any human HSD (503-505).”
From The Molecular Biology, Biochemistry, and Physiology of Human Steroidogenesis and Its Disorders (2011) · cites this paper as [503]
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- Authors
- Peltoketo, H., Isomaa, V., Mäentausta, O., & Vihko, R.
- Affiliations
- University of Oulu (FI)
- Corresponding author
- Peltoketo, H.
- ORCID
- Peltoketo, H.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1988-10-24
- Volume
- 239
- Issue
- 1
- Pages
- 73-77
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2073005394
- Cited by
- 294
- References
- 26
- Citations by year
- 2025: 2 · 2020: 1 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 2 · 2015: 1 · 2014: 4 · 2013: 1 · 2012: 3
- FWCI
- 4.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Keywords
- 17β-hydroxysteroid dehydrogenase, placenta, cDNA cloning, amino acid sequence, open reading frames, N-terminal sequence, steroid metabolism
- MeSH terms
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Female, Humans, Molecular Sequence Data, Placenta, Pregnancy, Protein Conformation, 17-Hydroxysteroid Dehydrogenases, Restriction Mapping
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