Origins of serotonergic projections to the spinal cord in rat: an immunocytochemical-retrograde transport study

Bowker, R.M.; Westlund, K.N.; Coulter, J.D.

doi:10.1016/0006-8993(81)91092-1

Cited by 317Neuroscience and Neuropharmacology ResearchNeurotransmitter Receptor Influence on BehaviorPain Mechanisms and Treatments

Origins of serotonergic projections to the spinal cord in rat: an immunocytochemical-retrograde transport study

Bowker, R.M., Westlund, K.N., Coulter, J.D.·1981·Brain Research

DOI 10.1016/0006-8993(81)91092-1

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Abstract

The origins of the serotonergic projections to the spinal cord in the rat were determined by employing the retrograde cell marker HRP coupled with the unlabeled antibody, peroxidase-antiperoxidase immunocytochemical method of Sternberger. Large numbers of stained neurons (greater than 70%) in the medullary raphe nuclear complex were found to contain both HRP retrogradely transported from the spinal cord and positive 5-HT staining. These serotonergic cell groups, including the nucleus raphe obscurus, raphe pallidus, raphe magnus, and the ventral parts of the reticular formation, project to all spinal cord levels. In addition, some neurons contained HRP granules, but were unstained for 5-HT, suggesting that they may contain other non-serotonergic neurotransmitters. More rostrally in the midbrain reticular formation, many 5-HT neurons were found to have projections exclusively to the cervical spinal cord. These findings indicate that the descending serotonin inputs to the spinal cord originate not only from the serotonergic neurons located in the medullary raphe complex, but also from other new sources located in the central gray and reticular formation of the midbrain.

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The rest of the DR serotonin neurons were divided into thalamus-projecting and brainstem/cerebellum-projecting groups based on the highest density of axons, one of which likely projecting to the spinal cord (brain 36) as previously reported.
“The rest of the DR serotonin neurons were divided into thalamus-projecting and brainstem/cerebellum-projecting groups based on the highest density of axons (Figure 8—figure supplement 3B,C), one of which likely projecting to the spinal cord (brain 36) as previously reported (Bowker et al., 1981; Bowker et al., 1982).”
From Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei (2019) · cites this paper as Bowker et al., 1981

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Authors: Bowker, R. M., Westlund, K. N., & Coulter, J. D.
Affiliations: The University of Texas Medical Branch at Galveston (US)
ORCID: Westlund, K.N.
Venue: Brain Research
Publisher: Elsevier BV
Published: 1981-12
Volume: 226
Issue: 1-2
Pages: 187-199
ISSN: 0006-8993 (ISSN-L)
DOI: 10.1016/0006-8993(81)91092-1
OpenAlex: W1976119196
Cited by: 317
References: 27
Citations by year: 2026: 2 · 2025: 1 · 2024: 2 · 2023: 4 · 2022: 6 · 2021: 2 · 2020: 2 · 2019: 4 · 2018: 3 · 2017: 3 · 2016: 3 · 2015: 6 · 2014: 5 · 2013: 6 · 2012: 5
FWCI: 6.03
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Brain Research (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health
Topics: Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
Health Sciences › Medicine › Physiology › Pain Mechanisms and Treatments
Keywords: serotonergic projections, spinal cord, nucleus raphe obscurus, nucleus raphe pallidus, nucleus raphe magnus, reticular formation, midbrain reticular formation, periaqueductal gray, serotonergic neurons, retrograde tracing, HRP
MeSH terms: Animals, Brain Stem, Horseradish Peroxidase, Immunoenzyme Techniques, Medulla Oblongata, Mesencephalon, Neural Pathways, Neurons, Pons, Raphe Nuclei, Reticular Formation, Serotonin, Spinal Cord, Rats

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Origins of serotonergic projections to the spinal cord in rat: an immunocytochemical-retrograde transport study

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