The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Non-enzymatic template-directed synthesis on RNA random copolymers
Abstract
Poly(C, U) random copolymer templates direct the oligomerization of 2-MeImpG and 2-MeImpA, resulting in the production of a variety of oligo/(G,A)s. The efficiency of monomer incorporation into newly synthesized oligomers is greater for 2-MeImpG than for 2-MeImpA, and decreases for both monomers as the uracil content of the template increases. The relatively poor incorporation of adenine is partly due to an intrinsically less efficient incorporation reaction, and partly due to the masking of uracil sites by G X U non-complementary pairing. The efficiency of adenine incorporation can be improved by decreasing the concentration of 2-MeImpG and increasing the concentration of 2-MeImpA in the reaction mixture. The oligomeric product distribution can be characterized in detail using high-pressure liquid chromatography on an RPC-5 column. Oligomers are separated on the basis of chain length, base composition, and phospho-diester-linkage isomerism. The 3'----5' regiospecificity of monomer addition to template-bound oligomers is lower for 2-MeImpA than for 2-MeImpG. The presence of an adenine residue at the 2'(3') terminus of the acceptor strand lowers the regiospecificity of 2-MeImpA addition even further.
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“Non-enzymatic RNA polymerization allows synthesis of short RNA oligonucleotides under prebiotic conditions (Joyce et al., 1984; Adamala and Szostak, 2013b).”
From The Astrobiology Primer v2.0 (2016) · cites this paper as Joyce et al., 1984
In the citing paper
“The prebiotic replication of RNA could have occurred non-enzymatically inside protocells via chemically activated nucleotides (Joyce et al., 1984; Mansy et al., 2008; Adamala and Szostak, 2013b).”
From The Astrobiology Primer v2.0 (2016) · cites this paper as Joyce et al., 1984
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Metadata
- Authors
- Joyce, G. F., Inoue, T., & Orgel, L. E.
- Affiliations
- Salk Institute for Biological Studies (US)
- ORCID
- Venue
- Journal of Molecular Biology
- Publisher
- Elsevier BV
- Published
- 1984-06
- Volume
- 176
- Issue
- 2
- Pages
- 279-306
- ISSN
- 0022-2836 (ISSN-L)
- OpenAlex
- W3184228035
- Cited by
- 178
- References
- 34
- Citations by year
- 2026: 1 · 2025: 4 · 2023: 3 · 2021: 2 · 2020: 4 · 2019: 5 · 2018: 2 · 2017: 4 · 2016: 8 · 2015: 6 · 2014: 5 · 2013: 4 · 2012: 5
- FWCI
- 2.51
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Molecular Biology (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Physical Sciences › Physics and Astronomy › Astronomy and Astrophysics › Origins and Evolution of Life
- Keywords
- oligonucleotide synthesis
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