Cited by 230HER2/EGFR in Cancer ResearchBreast Lesions and CarcinomasCancer-related Molecular Pathways

Amplification of the neu/erbB-2 oncogene in a mouse model of mammary tumorigenesis

Open access
Andrechek, Eran R., Hardy, William R., Siegel, Peter M., Rudnicki, Michael A., Cardiff, Robert D., Muller, William J.·2000·Proceedings of the National Academy of Sciences

Abstract

The neu (c-erbB-2, Her-2) protooncogene is amplified and overexpressed in 20-30% of human breast cancers. Although transgenic mouse models have illustrated the role of Neu in the induction of mammary tumors, Neu expression in these models is driven by a strong viral promoter of questionable relevance to the human disease. To ascertain whether expression of activated Neu under the control of the endogenous promoter in the mammary gland could induce mammary tumors we have generated mice that conditionally express activated Neu under the transcriptional control of the intact endogenous Neu promoter. Expression of oncogenic neu in the mammary gland resulted in accelerated lobulo-alveolar development and formation of focal mammary tumors after a long latency period. However, expression of activated Neu under the normal transcriptional control of the endogenous promoter was not sufficient for the initiation of mammary carcinogenesis. Strikingly, all mammary tumors bear amplified copies (2-22 copies) of the activated neu allele relative to the wild-type allele and express highly elevated levels of neu transcript and protein. Thus, like human erbB-2-positive breast tumors, mammary tumorigenesis in this mouse model requires the amplification and commensurate elevated expression of the neu gene.

Integrity signals

Registry

Crossref · 10.1073/pnas.97.7.3444

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Overexpression of wild-type Neu or HER2 under a mammary-specific promoter leads to metastatic mammary tumors in transgenic mice.

    “Consistent with its oncogenic activity, overexpression of wild-type Neu or HER2 under the control of a mammary-specific promoter leads to metastatic mammary tumors in transgenic mice (Andrechek et al., 2000; Finkle et al., 2004).”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Andrechek, E. R., Hardy, W. R., Siegel, P. M., Rudnicki, M. A., Cardiff, R. D., & Muller, W. J.
Affiliations
University of California, Davis (US)
Venue
Proceedings of the National Academy of Sciences
Published
2000-03-14
Volume
97
Issue
7
Pages
3444-3449
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2041576667
Cited by
230
References
38
Citations by year
2026: 5 · 2025: 4 · 2024: 5 · 2023: 7 · 2022: 2 · 2021: 6 · 2020: 10 · 2019: 7 · 2018: 6 · 2017: 7 · 2016: 8 · 2015: 9 · 2014: 12 · 2013: 9 · 2012: 8
FWCI
5.59
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Cancer Institute (U01CA105490)
Topics
  • Health Sciences › Medicine › Oncology › HER2/EGFR in Cancer Research
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Breast Lesions and Carcinomas
  • Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
Keywords
neu oncogene, c-erbB-2, HER2, gene amplification, mammary carcinogenesis, transgenic mouse model, conditional gene expression, endogenous promoter, breast cancer, oncogene overexpression
MeSH terms
Animals, Disease Models, Animal, Gene Amplification, Humans, Immunohistochemistry, Mammary Neoplasms, Experimental, Mice, Transgenic, DNA, Complementary, Genes, erbB-2, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review