Long-range single-molecule mapping of chromatin accessibility in eukaryotes
Open accessPublished version of a preprint
Abstract
Mapping open chromatin regions has emerged as a widely used tool for identifying active regulatory elements in eukaryotes. However, existing approaches, limited by reliance on DNA fragmentation and short-read sequencing, cannot provide information about large-scale chromatin states or reveal coordination between the states of distal regulatory elements. We have developed a method for profiling the accessibility of individual chromatin fibers, a single-molecule long-read accessible chromatin mapping sequencing assay (SMAC-seq), enabling the simultaneous, high-resolution, single-molecule assessment of chromatin states at multikilobase length scales. Our strategy is based on combining the preferential methylation of open chromatin regions by DNA methyltransferases with low sequence specificity, in this case EcoGII, an N 6-methyladenosine (m 6 A) methyltransferase, and the ability of nanopore sequencing to directly read DNA modifications. We demonstrate that aggregate SMAC-seq signals match bulk-level accessibility measurements, observe single-molecule nucleosome and transcription factor protection footprints, and quantify the correlation between chromatin states of distal genomic elements.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Single-molecule footprinting methods that use methyltransferases to mark accessible sites can provide quantitative measurements of binding states across individual molecules.
“single-molecule footprinting methods that use methyltransferases to mark accessible sites11–15 can provide quantitative measurements of these binding states across individual molecules”
From Single-molecule states link transcription factor binding to gene expression (2024) · cites this paper as [14]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome.
Langmead, B., Trapnell, C., Pop, M., & Salzberg, S. L. (2009) · Genome Biology · doi:10.1186/gb-2009-10-3-r25 · Cited by 21,849 · see citing works
Trapnell, C., Roberts, A., Goff, L. A., Pertea, G. M., Kim, D., Kelley, D. R., Pimentel, H., Salzberg, S. L., Rinn, J. L., & Pachter, L. S. (2012) · Nature Protocols · doi:10.1038/nprot.2012.016 · Cited by 11,930 · see citing works
MEME SUITE: tools for motif discovery and searching
Bailey, T. L., Boden, M., Buske, F. A., Frith, M., Grant, C. E., Clementi, L., Ren, J., Li, W. W., & Noble, W. S. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp335 · Cited by 10,648 · see citing works
Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome
Lieberman-Aiden, E., van Berkum, N. L., Williams, L., Imakaev, M., Ragoczy, T., Telling, A., Amit, I., Lajoie, B. R., Sabo, P. J., Dorschner, M. O., et al. (2009) · Science · doi:10.1126/science.1181369 · Cited by 8,898 · see citing works
Buenrostro, J. D., Giresi, P. G., Zaba, L. C., Chang, H. Y., & Greenleaf, W. J. (2013) · Nature Methods · doi:10.1038/nmeth.2688 · Cited by 6,422 · see citing works
Self-citationBismark: a flexible aligner and methylation caller for Bisulfite-Seq applications
Krueger, F., & Andrews, S. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr167 · Cited by 5,161 · see citing works
FIMO: scanning for occurrences of a given motif
Grant, C. E., Bailey, T. L., & Noble, W. S. (2011) · Bioinformatics · doi:10.1093/bioinformatics/btr064 · Cited by 4,247 · see citing works
An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues
Corces, M. R., Trevino, A. E., Hamilton, E. G., Greenside, P. G., Sinnott-Armstrong, N. A., Vesuna, S., Satpathy, A. T., Rubin, A. J., Montine, K. S., Wu, B., et al. (2017) · Nature Methods · doi:10.1038/nmeth.4396 · Cited by 2,496 · see citing works
Self-citationHighly Integrated Single-Base Resolution Maps of the Epigenome in Arabidopsis
Lister, R., O’Malley, R. C., Tonti‐Filippini, J., Gregory, B. D., Berry, C. C., Millar, A. H., & Ecker, J. R. (2008) · Cell · doi:10.1016/j.cell.2008.03.029 · Cited by 2,424 · see citing works
Identifying ChIP-seq enrichment using MACS
Feng, J., Liu, T., Qin, B., Zhang, Y., & Liu, X. S. (2012) · Nature Protocols · doi:10.1038/nprot.2012.101 · Cited by 1,805 · see citing works
Genome-Wide Evolutionary Analysis of Eukaryotic DNA Methylation
Zemach, A., McDaniel, I. E., Silva, P., & Zilberman, D. (2010) · Science · doi:10.1126/science.1186366 · Cited by 1,796 · see citing works
An oestrogen-receptor-α-bound human chromatin interactome
Fullwood, M. J., Liu, M. H., Pan, Y. F., Liu, J., Han, X., Mohamed, Y. B., Orlov, Y. L., Velkov, S., Ho, A., Poh, H. M., et al. (2009) · Nature · doi:10.1038/nature08497 · Cited by 1,728 · see citing works
Determination and Inference of Eukaryotic Transcription Factor Sequence Specificity
Weirauch, M. T., Yang, A. W. H., Albu, M., Coté, A. G., Montenegro‐Montero, A., Drewe, P., Najafabadi, H. S., Lambert, S. A., Mann, I. K., Cook, K. B., et al. (2014) · Cell · doi:10.1016/j.cell.2014.08.009 · Cited by 1,705 · see citing works
Minimap and miniasm: fast mapping and de novo assembly for noisy long sequences
Li, H. (2016) · Bioinformatics · doi:10.1093/bioinformatics/btw152 · Cited by 1,460 · see citing works
High-Resolution Mapping and Characterization of Open Chromatin across the Genome
Boyle, A. P., Davis, S., Shulha, H. P., Meltzer, P., Margulies, E. H., Weng, Z., Furey, T. S., & Crawford, G. E. (2008) · Cell · doi:10.1016/j.cell.2007.12.014 · Cited by 1,415 · see citing works
Dynamic Regulation of Nucleosome Positioning in the Human Genome
Schones, D. E., Cui, K., Cuddapah, S., Roh, T., Barski, A., Wang, Z., Wei, G., & Zhao, K. (2008) · Cell · doi:10.1016/j.cell.2008.02.022 · Cited by 1,396 · see citing works
Conservation and divergence of methylation patterning in plants and animals
Feng, S., Cokus, S. J., Zhang, X., Chen, P.-Y., Bostick, M., Goll, M. G., Hetzel, J., Jain, J., Strauss, S. H., Halpern, M. E., et al. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1002720107 · Cited by 1,285 · see citing works
HiChIP: efficient and sensitive analysis of protein-directed genome architecture
Mumbach, M. R., Rubin, A. J., Flynn, R. A., Dai, C., Khavari, P. A., Greenleaf, W. J., & Chang, H. Y. (2016) · Nature Methods · doi:10.1038/nmeth.3999 · Cited by 1,139 · see citing works
Self-citationThe 5′ ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I
Wu, C. C. M. (1980) · Nature · doi:10.1038/286854a0 · Cited by 1,118 · see citing works
Detecting DNA cytosine methylation using nanopore sequencing
Simpson, J. T., Workman, R. E., Zuzarte, P. C., David, M., Dursi, L. J., & Timp, W. (2017) · Nature Methods · doi:10.1038/nmeth.4184 · Cited by 1,109 · see citing works
Super-resolution imaging reveals distinct chromatin folding for different epigenetic states
Boettiger, A. N., Bintu, B., Moffitt, J. R., Wang, S. S., Beliveau, B. J., Fudenberg, G., Imakaev, M., Mirny, L. A., Wu, C.-T., & Zhuang, X. (2016) · Nature · doi:10.1038/nature16496 · Cited by 877 · see citing works
ChromEMT: Visualizing 3D chromatin structure and compaction in interphase and mitotic cells
Ou, H. D., Phan, S., Deerinck, T. J., Thor, A., Ellisman, M. H., & O’Shea, C. C. (2017) · Science · doi:10.1126/science.aag0025 · Cited by 872 · see citing works
Mapping Nucleosome Resolution Chromosome Folding in Yeast by Micro-C
Hsieh, T.-H. S., Weiner, A., Lajoie, B., Dekker, J., Friedman, N., & Rando, O. J. (2015) · Cell · doi:10.1016/j.cell.2015.05.048 · Cited by 788 · cited 1× · see citing works
“Analysis of Micro-C data 34 shows that significantly coaccessible promoters interact more frequently than non-coaccessible ones at a similar distance (Supplementary Fig.”
DNA Methylation on N6-Adenine in C. elegans
Greer, E. L., Blanco, M. A., Gu, L., Sendinc, E., Liu, J., Aristizábal-Corrales, D., Hsu, C.-H., Aravind, L., He, C., & Shi, Y. (2015) · Cell · doi:10.1016/j.cell.2015.04.005 · Cited by 702 · see citing works
The Response to Heat Shock and Oxidative Stress in Saccharomyces cerevisiae
Morano, K. A., Grant, C. M., & Moye-Rowley, S. (2011) · Genetics · doi:10.1534/genetics.111.128033 · Cited by 655 · see citing works
Global mapping of protein-DNA interactions in vivo by digital genomic footprinting
Hesselberth, J. R., Chen, X., Zhang, Z., Sabo, P. J., Sandstrom, R., Reynolds, A. P., Thurman, R. E., Neph, S., Kuehn, M. S., Noble, W. S., et al. (2009) · Nature Methods · doi:10.1038/nmeth.1313 · Cited by 559 · see citing works
N6-Methyldeoxyadenosine Marks Active Transcription Start Sites in Chlamydomonas
Fu, Y., Luo, G., Chen, K., Deng, X., Yu, M., Han, D., Hao, Z., Liu, J., Lu, X., Doré, L. C., et al. (2015) · Cell · doi:10.1016/j.cell.2015.04.010 · Cited by 517 · see citing works
Mapping DNA methylation with high-throughput nanopore sequencing
Rand, A. C., Jain, M., Eizenga, J. M., Musselman-Brown, A., Olsen, H. E., Akeson, M., & Paten, B. (2017) · Nature Methods · doi:10.1038/nmeth.4189 · Cited by 507 · see citing works
Genome-wide mapping of nucleosome positioning and DNA methylation within individual DNA molecules
Kelly, T. K., Liu, Y., Lay, F. D., Liang, G., Berman, B. P., & Jones, P. A. (2012) · Genome Research · doi:10.1101/gr.143008.112 · Cited by 455 · see citing works
A map of nucleosome positions in yeast at base-pair resolution
Brogaard, K., Xi, L., Wang, J.-P., & Widom, J. (2012) · Nature · doi:10.1038/nature11142 · Cited by 452 · see citing works
Extensive transcriptional heterogeneity revealed by isoform profiling
Pelechano, V., Wu, W., & Steinmetz, L. M. (2013) · Nature · doi:10.1038/nature12121 · Cited by 450 · see citing works
McGhee, J. D. (1981) · Cell · doi:10.1016/0092-8674(81)90359-7 · Cited by 437 · see citing works
Amplification-free whole-genome bisulfite sequencing by post-bisulfite adaptor tagging
Miura, F., Enomoto, Y., Dairiki, R., & Ito, T. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks454 · Cited by 419 · see citing works
The APOBEC Protein Family: United by Structure, Divergent in Function
Salter, J. D., Bennett, R. P., & Smith, H. C. (2016) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2016.05.001 · Cited by 402 · see citing works
Two different chromatin structures coexist in ribosomal RNA genes throughout the cell cycle
Conconi, A., Widmer, R. M., Koller, T., & Sogo, J. (1989) · Cell · doi:10.1016/0092-8674(89)90790-3 · Cited by 390 · see citing works
Fu, Y., Sinha, M., Peterson, C. L., & Weng, Z. (2008) · PLoS Genetics · doi:10.1371/journal.pgen.1000138 · Cited by 386 · see citing works
Schep, A. N., Buenrostro, J. D., Denny, S. K., Schwartz, K., Sherlock, G., & Greenleaf, W. J. (2015) · Genome Research · doi:10.1101/gr.192294.115 · Cited by 375 · see citing works
Self-citationThe interaction landscape between transcription factors and the nucleosome
Zhu, F., Farnung, L., Kaasinen, E., Sahu, B., Yin, Y., Wei, B., Dodonova, S., Nitta, K. R., Morgunova, E. Y., Taipale, M., et al. (2018) · Nature · doi:10.1038/s41586-018-0549-5 · Cited by 348 · see citing works
Gene loops juxtapose promoters and terminators in yeast
O'Sullivan, J. M., Tan‐Wong, S. M., Morillon, A., Lee, B., Coles, J., Mellor, J., & Proudfoot, N. J. (2004) · Nature Genetics · doi:10.1038/ng1411 · Cited by 346 · see citing works
French, S. L., Osheim, Y. N., Cioci, F., Nomura, M., & Beyer, A. L. (2003) · Molecular and Cellular Biology · doi:10.1128/mcb.23.5.1558-1568.2003 · Cited by 321 · see citing works
Genome-scale mapping of DNase I sensitivity in vivo using tiling DNA microarrays
Sabo, P. J., Kuehn, M. S., Thurman, R. E., Johnson, B., Johnson, E. M., Cao, H., Yu, M., Rosenzweig, E., Goldy, J., Haydock, A. K., et al. (2006) · Nature Methods · doi:10.1038/nmeth890 · Cited by 314 · see citing works
De novo Identification of DNA Modifications Enabled by Genome-Guided Nanopore Signal ProcessingPreprint
Stoiber, M. H., Quick, J., Egan, R. S., Lee, J. E., Celniker, S., Neely, R. K., Loman, N. J., Pennacchio, L. A., & Brown, J. B. (2016) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/094672 · Cited by 276 · see citing works
DNA N-Adenine Methylation in Arabidopsis thaliana
Liang, Z., Shen, L., Cui, X., Bao, S., Geng, Y., Yu, G., Liang, F., Xie, S. B., Lu, T., Gu, X., et al. (2018) · Developmental Cell · doi:10.1016/j.devcel.2018.03.012 · Cited by 259 · see citing works
Genome-wide Single-Molecule Footprinting Reveals High RNA Polymerase II Turnover at Paused Promoters
Krebs, A. R., Imanci, D., Hoerner, L., Gaidatzis, D., Burger, L., & Schübeler, D. (2017) · Molecular Cell · doi:10.1016/j.molcel.2017.06.027 · Cited by 249 · see citing works
Gene Loops Enhance Transcriptional Directionality
Tan‐Wong, S. M., Zaugg, J. B., Camblong, J., Xu, Z., Zhang, D. W., Mischo, H. E., Ansari, A. Z., Luscombe, N. M., Steinmetz, L. M., & Proudfoot, N. J. (2012) · Science · doi:10.1126/science.1224350 · Cited by 241 · see citing works
Huang, J., & Moazed, D. (2003) · Genes & Development · doi:10.1101/gad.1108403 · Cited by 232 · see citing works
Keene, M. A., Corces, V. G., Lowenhaupt, K., & Elgin, S. C. R. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.1.143 · Cited by 214 · see citing works
High-Resolution Chromatin Dynamics during a Yeast Stress Response
Weiner, A., Hsieh, T.-H. S., Chappleboim, A., Chen, H. V., Rahat, A., Amit, I., Rando, O. J., & Friedman, N. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.02.002 · Cited by 205 · see citing works
Merz, K., Hondele, M., Goetze, H., Gmelch, K., Stöckl, U., & Griesenbeck, J. (2008) · Genes & Development · doi:10.1101/gad.466908 · Cited by 173 · see citing works
Precise genome-wide mapping of single nucleosomes and linkers in vivo
Chereji, R. V., Ramachandran, S., Bryson, T. D., & Henikoff, S. (2018) · Genome biology · doi:10.1186/s13059-018-1398-0 · Cited by 160 · see citing works
Variable chromatin structure revealed by in situ spatially correlated DNA cleavage mapping
Risca, V. I., Denny, S. K., Straight, A. F., & Greenleaf, W. J. (2016) · Nature · doi:10.1038/nature20781 · Cited by 159 · see citing works
Self-citationHu, B., Petela, N. J., Kurze, A., Chan, K.-L., Chapard, C., & Nasmyth, K. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv670 · Cited by 155 · see citing works
High-throughput localization of functional elements by quantitative chromatin profiling
Dorschner, M. O., Hawrylycz, M., Humbert, R., Wallace, J. C., Shafer, A., Kawamoto, J., Mack, J., Hall, R., Goldy, J., Sabo, P. J., et al. (2004) · Nature Methods · doi:10.1038/nmeth721 · Cited by 133 · see citing works
Wang, Y., Chen, X., Sheng, Y., Liu, Y., & Gao, S. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx883 · Cited by 129 · see citing works
Gabrieli, T., Sharim, H., Fridman, D., Arbib, N., Michaeli, Y., & Ebenstein, Y. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky411 · Cited by 123 · see citing works
Timinskas, A., Butkus, V., & Janulaitis, A. (1995) · Gene · doi:10.1016/0378-1119(94)00783-o · Cited by 106 · see citing works
Asymmetric nucleosomes flank promoters in the budding yeast genomePreprint
Ramachandran, S., Zentner, G. E., & Henikoff, S. (2014) · Genome Research · doi:10.1101/gr.182618.114 · Cited by 96 · see citing works
Henikoff, S., Ramachandran, S., Krassovsky, K., Bryson, T. D., Codomo, C. A., Brogaard, K., Widom, J., Wang, J.-P., & Henikoff, J. G. (2014) · eLife · doi:10.7554/elife.01861 · Cited by 88 · see citing works
The centromeric nucleosome of budding yeast is perfectly positioned and covers the entire centromere
Cole, H. A., Howard, B. H., & Clark, D. J. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1104978108 · Cited by 83 · see citing works
Genome-wide measurement of local nucleosome array regularity and spacing by nanopore sequencing
Baldi, S., Krebs, S., Blum, H., & Becker, P. B. (2018) · Nature Structural & Molecular Biology · doi:10.1038/s41594-018-0110-0 · Cited by 74 · see citing works
RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA
Jones, H. S., Kawauchi, J., Braglia, P., Amaro, C. A., Kent, N. A., & Proudfoot, N. J. (2007) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1199 · Cited by 73 · see citing works
N6-methyldeoxyadenosine directs nucleosome positioning in Tetrahymena DNA
Luo, G., Hao, Z., Luo, L., Shen, M., Sparvoli, D., Zheng, Y., Zhang, Z. S., Weng, X., Chen, K., Cui, Q., et al. (2018) · Genome biology · doi:10.1186/s13059-018-1573-3 · Cited by 62 · see citing works
Genome-wide mapping of 5-hydroxymethyluracil in the eukaryote parasite Leishmania
Kawasaki, F., Beraldi, D., Hardisty, R. E., McInroy, G. R., van Delft, P., & Balasubramanian, S. (2017) · Genome biology · doi:10.1186/s13059-017-1150-1 · Cited by 59 · see citing works
Nabilsi, N. H., Deleyrolle, L. P., Darst, R. P., Riva, A., Reynolds, B. A., & Kladde, M. P. (2013) · Genome Research · doi:10.1101/gr.161737.113 · Cited by 53 · see citing works
The non-specific adenine DNA methyltransferase M.EcoGII
Murray, I. A., Morgan, R., Luyten, Y. A., Fomenkov, A. I., Junior, I. R. C., Dai, N., Allaw, M. B., Zhang, X., Cheng, X., & Roberts, R. J. (2017) · Nucleic Acids Research · doi:10.1093/nar/gkx1191 · Cited by 49 · see citing works
Goetze, H., Wittner, M., Hamperl, S., Hondele, M., Merz, K., Stöckl, U., & Griesenbeck, J. (2010) · Molecular and Cellular Biology · doi:10.1128/mcb.01512-09 · Cited by 44 · see citing works
Yeast HMO1: Linker Histone Reinvented
Panday, A., & Grove, A. (2016) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00037-16 · Cited by 42 · see citing works
Staševskij, Z., Gibas, P., Gordevičius, J., Kriukienė, E., & Klimašauskas, S. (2017) · Molecular Cell · doi:10.1016/j.molcel.2016.12.012 · Cited by 34 · see citing works
The current status of liver transplantation.
Calne, R. Y. (1986) · PubMed · Cited by 23 · see citing works
Effect of Seizures on Brain Phenobarbital Concentration in Newborn Piglets
Monin, P., Clozel, M., Morselli, P. L., Dubruc, C., & Vert, P. (1987) · Epilepsia · doi:10.1111/j.1528-1157.1987.tb03644.x · Cited by 14 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Nanopore sequencing technology, bioinformatics and applications
Wang, Y., Zhao, Y., Bollas, A. E., Wang, Y., & Au, K. F. (2021) · Nature Biotechnology · doi:10.1038/s41587-021-01108-x · Cited by 1,756 · see citing works
Single-molecule regulatory architectures captured by chromatin fiber sequencing
Stergachis, A. B., Debo, B. M., Haugen, E., Churchman, L. S., & Stamatoyannopoulos, J. A. (2020) · Science · doi:10.1126/science.aaz1646 · Cited by 257 · see citing works
Epigenetic patterns in a complete human genome
Gershman, A., Sauria, M. E. G., Guitart, X., Vollger, M. R., Hook, P. W., Hoyt, S. J., Jain, M., Shumate, A., Razaghi, R., Koren, S., et al. (2022) · Science · doi:10.1126/science.abj5089 · Cited by 297 · see citing works
Lee, I., Razaghi, R., Gilpatrick, T., Molnar, M. M., Gershman, A., Sadowski, N., Sedlazeck, F. J., Hansen, K. D., Simpson, J. T., & Timp, W. (2020) · Nature Methods · doi:10.1038/s41592-020-01000-7 · Cited by 212 · see citing works
Chromatin accessibility profiling methods
Minnoye, L., Marinov, G. K., Krausgruber, T., Pan, L., Marand, A. P., Secchia, S., Greenleaf, W. J., Furlong, E. E. M., Zhao, K., Schmitz, R. J., et al. (2021) · Nature Reviews Methods Primers · doi:10.1038/s43586-020-00008-9 · Cited by 206 · see citing works
Self-citationMolecular Co-occupancy Identifies Transcription Factor Binding Cooperativity In Vivo
Sönmezer, C., Kleinendorst, R., Imanci, D., Barzaghi, G., Villacorta, L., Schübeler, D., Benes, V., Molina, N., & Krebs, A. R. (2021) · Molecular Cell · doi:10.1016/j.molcel.2020.11.015 · Cited by 162 · see citing works
Generating specificity in genome regulation through transcription factor sensitivity to chromatin
Isbel, L., Grand, R. S., & Schübeler, D. (2022) · Nature Reviews Genetics · doi:10.1038/s41576-022-00512-6 · Cited by 149 · see citing works
DiMeLo-seq: a long-read, single-molecule method for mapping protein–DNA interactions genome wide
Altemose, N., Maslan, A., Smith, O. K., Sundararajan, K., Brown, R. R., Mishra, R., Detweiler, A. M., Neff, N. F., Miga, K. H., Straight, A. F., et al. (2022) · Nature Methods · doi:10.1038/s41592-022-01475-6 · Cited by 154 · see citing works
Recent advances in the detection of base modifications using the Nanopore sequencer
Xu, L., & Seki, M. (2019) · Journal of Human Genetics · doi:10.1038/s10038-019-0679-0 · Cited by 153 · see citing works
Chromatin accessibility: methods, mechanisms, and biological insights
Mansisidor, A., & Risca, V. I. (2022) · Nucleus · doi:10.1080/19491034.2022.2143106 · Cited by 150 · see citing works
Recent advances in single-cell sequencing technologies
Wen, L., & Tang, F. (2022) · Precision Clinical Medicine · doi:10.1093/pcmedi/pbac002 · Cited by 127 · see citing works
Targeted profiling of human extrachromosomal DNA by CRISPR-CATCH
Hung, K. L., Luebeck, J., Dehkordi, S. R., Colón, C. I., Li, R., Wong, I. T.-L., Çoruh, C., Dharanipragada, P., Lomeli, S. H., Weiser, N. E., et al. (2022) · Nature Genetics · doi:10.1038/s41588-022-01190-0 · Cited by 135 · see citing works
Self-citationGenomics in the long-read sequencing era
van Dijk, E., Naquin, D., Gorrichon, K., Jaszczyszyn, Y., Ouazahrou, R., Thermes, C., & Hernandez, C. (2023) · Trends in Genetics · doi:10.1016/j.tig.2023.04.006 · Cited by 134 · see citing works
Massively multiplex single-molecule oligonucleosome footprinting
Abdulhay, N. J., McNally, C. P., Hsieh, L. J., Kasinathan, S., Keith, A., Estes, L. S., Karimzadeh, M., Underwood, J. G., Goodarzi, H., Narlikar, G. J., et al. (2020) · eLife · doi:10.7554/elife.59404 · Cited by 108 · see citing works
Single-cell sequencing technology applied to epigenetics for the study of tumor heterogeneity
Hu, Y., Shen, F., Yang, X., Han, T., Long, Z., Wen, J., Huang, J., Shen, J., & Guo, Q. (2023) · Clinical Epigenetics · doi:10.1186/s13148-023-01574-x · Cited by 95 · see citing works
RNA polymerase II dynamics and mRNA stability feedback scale mRNA amounts with cell size
Swaffer, M. P., Marinov, G. K., Zheng, H., Valenzuela, L. F., Tsui, C. Y., Jones, A. W., Greenwood, J., Kundaje, A. B., Greenleaf, W. J., Reyes‐Lamothe, R., et al. (2023) · Cell · doi:10.1016/j.cell.2023.10.012 · Cited by 79 · see citing works
Self-citationNavigating the pitfalls of mapping DNA and RNA modifications
Kong, Y., Mead, E. A., & Fang, G. (2023) · Nature Reviews Genetics · doi:10.1038/s41576-022-00559-5 · Cited by 85 · see citing works
Methylartist: tools for visualizing modified bases from nanopore sequence data
Cheetham, S. W., Kindlova, M., & Ewing, A. D. (2022) · Bioinformatics · doi:10.1093/bioinformatics/btac292 · Cited by 72 · see citing works
Long-range phasing of dynamic, tissue-specific and allele-specific regulatory elements
Battaglia, S., Dong, K., Wu, J., Chen, Z., Najm, F. J., Zhang, Y., Moore, M. M., Hecht, V. C., Noam Shoresh, F., & Bernstein, B. (2022) · Nature Genetics · doi:10.1038/s41588-022-01188-8 · Cited by 65 · see citing works
Beyond assembly: the increasing flexibility of single-molecule sequencing technology
Hook, P. W., & Timp, W. (2023) · Nature Reviews Genetics · doi:10.1038/s41576-023-00600-1 · Cited by 67 · see citing works
Single-nucleoid architecture reveals heterogeneous packaging of mitochondrial DNA
Isaac, R. S., Tullius, T. W., Hansen, K. G., Dubocanin, D., Couvillion, M., Stergachis, A. B., & Churchman, L. S. (2024) · Nature Structural & Molecular Biology · doi:10.1038/s41594-024-01225-6 · Cited by 65 · see citing works
DNA-m6A calling and integrated long-read epigenetic and genetic analysis with fibertoolsPreprint
Jha, A., Bohaczuk, S. C., Mao, Y., Ranchalis, J., Mallory, B. J., Min, A., Hamm, M. O., Swanson, E. G., Dubocanin, D., Finkbeiner, C., et al. (2024) · Genome Research · doi:10.1101/gr.279095.124 · Cited by 58 · see citing works
Centromere studies in the era of ‘telomere-to-telomere’ genomics
Miga, K. H. (2020) · Experimental Cell Research · doi:10.1016/j.yexcr.2020.112127 · Cited by 63 · see citing works
Modification mapping by nanopore sequencing
White, L. K., & Hesselberth, J. R. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.1037134 · Cited by 58 · see citing works
Transcriptional and chromatin-based partitioning mechanisms uncouple protein scaling from cell size
Swaffer, M. P., Kim, J., Chandler-Brown, D. B., Langhinrichs, M., Marinov, G. K., Greenleaf, W. J., Kundaje, A. B., Schmoller, K. M., & Skotheim, J. M. (2021) · Molecular Cell · doi:10.1016/j.molcel.2021.10.007 · Cited by 54 · see citing works
Self-citationLv, H., Dao, F., Zhang, D., Yang, H. Q., & Lin, H. (2021) · Biotechnology and Bioengineering · doi:10.1002/bit.27911 · Cited by 58 · see citing works
Profiling the epigenome using long-read sequencing
Liu, T., & Conesa, A. (2025) · Nature Genetics · doi:10.1038/s41588-024-02038-5 · Cited by 57 · see citing works
Nucleosome Positioning and Spacing: From Mechanism to Function
Singh, A. K., & Mueller‐Planitz, F. (2021) · Journal of Molecular Biology · doi:10.1016/j.jmb.2021.166847 · Cited by 54 · see citing works
Vollger, M. R., Korlach, J., Eldred, K. C., Swanson, E. G., Underwood, J. G., Bohaczuk, S. C., Mao, Y., Cheng, Y.-H. H., Ranchalis, J. E., Blue, E., et al. (2025) · Nature Genetics · doi:10.1038/s41588-024-02067-0 · Cited by 48 · see citing works
Single-molecule states link transcription factor binding to gene expression
Doughty, B. R., Hinks, M. M., Schaepe, J. M., Marinov, G. K., Thurm, A. R., Rios-Martinez, C., Parks, B. E., Tan, Y., Marklund, E., Dubocanin, D., et al. (2024) · Nature · doi:10.1038/s41586-024-08219-w · Cited by 42 · see citing works
Self-citationInterplay among ATP-Dependent Chromatin Remodelers Determines Chromatin Organisation in Yeast
Prajapati, H. K., Ocampo, J., & Clark, D. J. (2020) · Biology · doi:10.3390/biology9080190 · Cited by 40 · see citing works
Nucleosome density shapes kilobase-scale regulation by a mammalian chromatin remodeler
Abdulhay, N. J., Hsieh, L. J., McNally, C. P., Ostrowski, M. S., Moore, C. M., Ketavarapu, M., Kasinathan, S., Nanda, A. S., Wu, K., Chio, U. S., et al. (2023) · Nature Structural & Molecular Biology · doi:10.1038/s41594-023-01093-6 · Cited by 38 · see citing works
Bonev, B., Castelo‐Branco, G., Chen, F., Codeluppi, S., Corces, M. R., Fan, J., Heiman, M., Harris, K., Inoue, F., Kellis, M., et al. (2024) · Nature Neuroscience · doi:10.1038/s41593-024-01806-0 · Cited by 39 · see citing works
Lim, D. Y., Khanal, J., Tayara, H., & Chong, K. T. (2021) · Chemometrics and Intelligent Laboratory Systems · doi:10.1016/j.chemolab.2021.104284 · Cited by 37 · see citing works
Revisiting chromatin packaging in mouse spermPreprint
Yin, Q., Yang, C.-H., Strelkova, O. S., Wu, J., Sun, Y. H., Gopalan, S., Yang, L., Dekker, J., Fazzio, T. G., Li, X. Z., et al. (2023) · Genome Research · doi:10.1101/gr.277845.123 · Cited by 38 · see citing works
The single-molecule accessibility landscape of newly replicated mammalian chromatin
Ostrowski, M. S., Yang, M. G., McNally, C. P., Abdulhay, N. J., Wang, S., Renduchintala, K., Irkliyenko, I., Biran, A., Chew, B. T. L., Midha, A. D., et al. (2024) · Cell · doi:10.1016/j.cell.2024.10.039 · Cited by 36 · see citing works
Long-read sequencing to understand genome biology and cell function
Kraft, F., & Kurth, I. (2020) · The International Journal of Biochemistry & Cell Biology · doi:10.1016/j.biocel.2020.105799 · Cited by 37 · see citing works
Chen, W., Weng, Z., Xie, Z., Xie, Y., Zhang, C., Chen, Z., Ruan, F., Wang, J., Sun, Y., Fang, Y., et al. (2021) · Epigenetics & Chromatin · doi:10.1186/s13072-021-00416-5 · Cited by 34 · see citing works
Direct transposition of native DNA for sensitive multimodal single-molecule sequencing
Nanda, A. S., Wu, K., Irkliyenko, I., Woo, B., Ostrowski, M. S., Clugston, A. S., Sayles, L. C., Xu, L., Satpathy, A. T., Nguyen, H. G., et al. (2024) · Nature Genetics · doi:10.1038/s41588-024-01748-0 · Cited by 35 · see citing works
RNA polymerases reshape chromatin architecture and couple transcription on individual fibers
Tullius, T. W., Isaac, R. S., Dubocanin, D., Ranchalis, J., Churchman, L. S., & Stergachis, A. B. (2024) · Molecular Cell · doi:10.1016/j.molcel.2024.08.013 · Cited by 31 · see citing works
Zhang, J., Xie, S., Xu, J., Liu, H., & Wan, S. (2021) · Frontiers in Genetics · doi:10.3389/fgene.2021.672804 · Cited by 31 · see citing works
Interrogating the Accessible Chromatin Landscape of Eukaryote Genomes Using ATAC-seq
Marinov, G. K., & Shipony, Z. (2021) · Methods in molecular biology · doi:10.1007/978-1-0716-1103-6_10 · Cited by 30 · see citing works
Self-citationStudying transcription factor function in the genome at molecular resolution
Krebs, A. R. (2021) · Trends in Genetics · doi:10.1016/j.tig.2021.03.008 · Cited by 27 · see citing works
Somatic epimutations cap genetic determinism in the human diploid chromatin epigenomePreprint
Vollger, M. R., Swanson, E. G., Neph, S. J., Mallory, B. J., Dubocanin, D., Ho, C., Cheng, Y. H. H., Ranchalis, J. E., Munson, K. M., Sedeño-Cortés, A. E., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.06.14.599122 · Cited by 30 · see citing works
Human cell transformation by combined lineage conversion and oncogene expression
Sahu, B., Pihlajamaa, P., Zhang, K., Palin, K., Ahonen, S., Cervera, A., Ristimaki, A. P., Aaltonen, L. A., Hautaniemi, S., & Taipale, J. (2021) · Oncogene · doi:10.1038/s41388-021-01940-0 · Cited by 30 · see citing works
Brothers, M., & Rine, J. (2022) · eLife · doi:10.7554/elife.75653 · Cited by 29 · see citing works
Li, Y., Xu, J., Chen, C., Lu, Z., Wan, D., Li, D., Li, J. S., Sorg, A. J., Roberts, C. C., Mahajan, S., et al. (2024) · Genome Medicine · doi:10.1186/s13073-023-01280-6 · Cited by 28 · see citing works
Genome-in-a-Box: Building a Chromosome from the Bottom Up
Birnie, A., & Dekker, C. (2020) · ACS Nano · doi:10.1021/acsnano.0c07397 · Cited by 28 · see citing works
Leduque, B., Edera, A., Vitte, C., & Quadrana, L. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae306 · Cited by 26 · see citing works
Conservation of dichromatin organization along regional centromeres
Dubocanin, D., Hartley, G. A., Sedeño-Cortés, A. E., Mao, Y., Hédouin, S., Ranchalis, J., Agarwal, A., Logsdon, G. A., Munson, K. M., Real, T. D., et al. (2025) · Cell Genomics · doi:10.1016/j.xgen.2025.100819 · Cited by 26 · see citing works
Mo, W., Yi, S., Liu, B., Long, Y., Li, T., Cao, X., Deng, X. M., & Zhai, J. (2023) · Nature Plants · doi:10.1038/s41477-023-01498-7 · Cited by 27 · see citing works
Weng, Z., Ruan, F., Chen, W., Chen, Z., Xie, Y., Luo, M., Xie, Z., Zhang, C., Wang, J., Sun, Y., et al. (2023) · Genome biology · doi:10.1186/s13059-023-02896-y · Cited by 24 · see citing works
Noack, F., Vangelisti, S., Ditzer, N., Chong, F., Albert, M., & Bonev, B. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01296-5 · Cited by 26 · see citing works
Kleinendorst, R., Barzaghi, G., Smith, M. L., Zaugg, J. B., & Krebs, A. R. (2021) · Nature Protocols · doi:10.1038/s41596-021-00630-1 · Cited by 23 · see citing works
The Active Mechanism of Nucleosome Depletion by Poly(dA:dT) Tracts In Vivo
Barnes, T., & Korber, P. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22158233 · Cited by 22 · see citing works
A single fiber view of the nucleosome organization in eukaryotic chromatin
Boltengagen, M., Verhagen, D. G. F., Wolff, M. R., Oberbeckmann, E., Hanke, M., Gerland, U., Korber, P., & Mueller‐Planitz, F. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad1098 · Cited by 21 · see citing works
Mapping single-cell diploid chromatin fiber architectures using DAF-seq
Swanson, E. G., Mao, Y.-Z., Mallory, B., Vollger, M., Bohaczuk, S. C., Oliveira, C. B., Lyon, D., Ranchalis, J., Parmalee, N. L., Cohen, B. A., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-025-02914-3 · Cited by 11 · see citing works
Semiconservative transmission of DNA N 6 -adenine methylation in a unicellular eukaryotePreprint
Sheng, Y., Wang, Y., Yang, W., Wang, X. Q. D., Lu, J., Pan, B., Nan, B., Liu, Y., Ye, F., Li, C., et al. (2024) · Genome Research · doi:10.1101/gr.277843.123 · Cited by 18 · see citing works
Mapping protein–DNA interactions with DiMeLo-seq
Maslan, A., Altemose, N., Marcus, J., Mishra, R., Brennan, L. D., Sundararajan, K., Karpen, G. H., Straight, A. F., & Streets, A. (2024) · Nature Protocols · doi:10.1038/s41596-024-01032-9 · Cited by 16 · see citing works
On the role of transcription in positioning nucleosomes
Jiang, Z., & Zhang, B. (2021) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1008556 · Cited by 17 · see citing works
Doughty, B. R., Hinks, M. M., Schaepe, J. M., Marinov, G., Thurm, A. R., Rios-Martinez, C., Parks, B. E., Tan, Y.-X., Marklund, E., Dubocanin, D., et al. (2024) · bioRxiv · doi:10.1101/2024.02.02.578660 · Cited by 14 · see citing works
Self-citationXie, Y., Ruan, F., Li, Y., Luo, M., Zhang, C., Chen, Z., Xie, Z., Weng, Z., Chen, W., Chen, W., et al. (2023) · eLife · doi:10.7554/elife.87868 · Cited by 16 · see citing works
Transposable elements as genome regulators in normal and malignant haematopoiesis
Prokopov, D., Tunbak, H., Leddy, E., Drylie, B., Camera, F., & Deniz, Ö. (2025) · Blood Cancer Journal · doi:10.1038/s41408-025-01295-9 · Cited by 14 · see citing works
Fukai, Y. T., Kujirai, T., Wakamori, M., Kanamura, S., Yamauchi, L., Zeraati, S., Morita, S., Tanegashima, C., Kadota, M., Shirouzu, M., et al. (2025) · Science Advances · doi:10.1126/sciadv.adx9282 · Cited by 14 · see citing works
Wu, S., Tao, T., Zhang, L., Zhu, X. M., & Zhou, X. (2023) · Heliyon · doi:10.1016/j.heliyon.2023.e21327 · Cited by 15 · see citing works
Insight on ecDNA-mediated tumorigenesis and drug resistance
Huang, Q., Zhang, S., Wang, G., & Han, J. (2024) · Heliyon · doi:10.1016/j.heliyon.2024.e27733 · Cited by 14 · see citing works
Effective dynamics of nucleosome configurations at the yeast PHO5 promoter
Wolff, M. R., Schmid, A., Korber, P., & Gerland, U. (2021) · eLife · doi:10.7554/elife.58394 · Cited by 12 · see citing works
Single-Molecule Techniques to Study Chromatin
Chanou, A., & Hamperl, S. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.699771 · Cited by 14 · see citing works
Fu, H., Zheng, H., Chen, X., Weirauch, M. T., Muglia, L. J., Wang, L., & Liu, Y. (2023) · Genome biology · doi:10.1186/s13059-023-02889-x · Cited by 12 · see citing works
Synthetic promoter design in plants: integration of computational and experimental approaches
Yaschenko, A. E., Alonso, J. M., & Stepanova, A. N. (2026) · Frontiers in Plant Science · doi:10.3389/fpls.2026.1768521 · Cited by 12 · see citing works
Dennis, A. F., Xu, Z., & Clark, D. J. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae288 · Cited by 11 · see citing works
Marinov, G. K., Chen, X., Swaffer, M. P., Xiang, T., Grossman, A. R., & Greenleaf, W. J. (2024) · Genome biology · doi:10.1186/s13059-024-03261-3 · Cited by 11 · see citing works
Self-citationGenome-Wide Mapping of Active Regulatory Elements Using ATAC-seq
Marinov, G. K., Shipony, Z., Kundaje, A. B., & Greenleaf, W. J. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2899-7_1 · Cited by 11 · see citing works
Self-citationCheetham, S. W., Jafrani, Y., Andersen, S. B., Jansz, N., Kindlova, M., Ewing, A. D., & Faulkner, G. J. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.08.09.455753 · Cited by 11 · see citing works
Resolving the chromatin impact of mosaic variants with targeted Fiber-seqPreprint
Bohaczuk, S. C., Amador, Z. J., Li, C. L., Mallory, B. J., Swanson, E. G., Ranchalis, J., Vollger, M. R., Munson, K. M., Walsh, T., Hamm, M. O., et al. (2024) · Genome Research · doi:10.1101/gr.279747.124 · Cited by 11 · see citing works
Roh, H., Shen, S. P., Yan, H., Kwok, H. S., Siegenfeld, A. P., Lee, C., Zepeda, M. L., Guo, C., Roseman, S. A., Comenho, C., et al. (2025) · Nature Methods · doi:10.1038/s41592-025-02811-2 · Cited by 9 · see citing works
The chromatin landscape of the euryarchaeon Haloferax volcanii
Marinov, G. K., Bagdatli, S. T., Wu, T., He, C., Kundaje, A. B., & Greenleaf, W. J. (2023) · Genome biology · doi:10.1186/s13059-023-03095-5 · Cited by 8 · see citing works
Self-citationLong-range single-molecule mapping of chromatin modification in eukaryotesPreprint
Weng, Z., Ruan, F., Chen, W., Xie, Z., Xie, Y., Zhang, C., Chen, Z., Wang, J., Sun, Y., Fang, Y., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.07.08.451578 · Cited by 6 · see citing works
Methylartist: Tools for Visualising Modified Bases from Nanopore Sequence DataPreprint
Cheetham, S. W., Kindlova, M., & Ewing, A. D. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.07.22.453313 · Cited by 9 · see citing works
SUPREM: an engineered non-site-specific m6A RNA methyltransferase with highly improved efficiency
Ochiai, Y., Clifton, B. E., Le Coz, M., Terenzio, M., & Laurino, P. (2024) · Nucleic Acids Research · doi:10.1093/nar/gkae887 · Cited by 9 · see citing works
Summary of ChIP-Seq Methods and Description of an Optimized ChIP-Seq Protocol
Fadri, M. T. M., Lee, J. B., & Keung, A. J. (2024) · Methods in molecular biology · doi:10.1007/978-1-0716-4051-7_22 · Cited by 8 · see citing works
The chromatin organization of a chlorarachniophyte nucleomorph genome
Marinov, G. K., Chen, X., Wu, T., He, C., Grossman, A. R., Kundaje, A. B., & Greenleaf, W. J. (2022) · Genome biology · doi:10.1186/s13059-022-02639-5 · Cited by 8 · see citing works
Self-citationSingle chromatin fiber profiling and nucleosome position mapping in the human brain
Peter, C. J., Agarwal, A., Watanabe, R., Kassim, B. S., Wang, X., Lambert, T. Y., Javidfar, B., Evans, V. D., Dawson, T., Fridrikh, M., et al. (2024) · Cell Reports Methods · doi:10.1016/j.crmeth.2024.100911 · Cited by 7 · see citing works
Mouse promoters are characterised by low occupancy and high turnover of RNA polymerase II
Chatsirisupachai, K., Moene, C. J. I., Kleinendorst, R., Kreibich, E., Molina, N., & Krebs, A. R. (2025) · Molecular Systems Biology · doi:10.1038/s44320-025-00094-5 · Cited by 7 · see citing works
Nifker, G., Grunwald, A., Margalit, S., Tulpová, Z., Michaeli, Y., Har-Gil, H., Maimon, N., Roichman, E., Schütz, L., Weinhold, E. G., et al. (2023) · ACS Nano · doi:10.1021/acsnano.2c12755 · Cited by 7 · see citing works
Single molecule MATAC-seq reveals key determinants of DNA replication origin efficiency
Chanou, A., Weiβ, M., Holler, K., Sajid, A., Straub, T., Krietsch, J., Sanchi, A., Ummethum, H., Lee, C. S. K., Kruse, E., et al. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad1022 · Cited by 6 · see citing works
Marinov, G. K., Shipony, Z., Kundaje, A. B., & Greenleaf, W. J. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2140-0_15 · Cited by 6 · see citing works
Self-citationSemi-conservative transmission of DNA N 6 -adenine methylation in a unicellular eukaryotePreprint
Sheng, Y., Wang, Y., Yang, W., Wang, X. Q. D., Lu, J., Pan, B., Nan, B., Liu, Y., Li, C., Song, J., et al. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.02.15.468708 · Cited by 6 · see citing works
Deaminase-mediated chromatin accessibility profiling with single-allele resolutionPreprint
Yu, T., Li, Z., Gibbs, E., Iwase, R., Francoeur, M., Phan, Q. V., Zhao, J., Rosin, J., Cole, P. A., Pinello, L., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.12.17.628768 · Cited by 6 · see citing works
Baderna, V., Barzaghi, G., Kleinendorst, R., Chatsirisupachai, K., Moniot-Perron, L., Doyle, C., Schopp, M., Hochepied, T., Libert, C., Odom, D. T., et al. (2026) · Nature Genetics · doi:10.1038/s41588-026-02703-x · Cited by 7 · see citing works
Electrochemical Biosensing Methods for Detecting Epigenetic Modifications
Huang, Z., Dou, Y., Su, J., Li, T., & Song, S. (2023) · Chemosensors · doi:10.3390/chemosensors11080424 · Cited by 5 · see citing works
Abdulhay, N. J., Hsieh, L. J., McNally, C. P., Ketavarapu, M., Kasinathan, S., Nanda, A. S., Ostrowski, M. S., Wu, K., Moore, C. M., Goodarzi, H., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.12.10.472156 · Cited by 5 · see citing works
Mass Spectrometry to Study Chromatin Compaction
Stransky, S., Aguilan, J. T., Lachowicz, J. C., Madrid-Aliste, C. J., Nieves, E., & Sidoli, S. (2020) · Biology · doi:10.3390/biology9060140 · Cited by 4 · see citing works
The Active Rather than Intrinsic Mechanism of Nucleosome Depletion by Poly(dA:dT) TractsPreprint
Barnes, T., & Korber, P. (2021) · Preprints.org · doi:10.20944/preprints202104.0579.v1 · Cited by 4 · see citing works
Chen, W., Weng, Z., Xie, Z., Xie, Y., Zhang, C., Chen, Z., Ruan, F., Wang, J., Sun, Y., Fang, Y., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.03.31.437970 · Cited by 4 · see citing works
The chromatin landscape of the histone-possessingBacteriovoraxbacteriaPreprint
Marinov, G. K., Doughty, B. R., Kundaje, A. B., & Greenleaf, W. J. (2024) · Genome Research · doi:10.1101/gr.279418.124 · Cited by 4 · see citing works
Self-citationKAS-ATAC reveals the genome-wide single-stranded accessible chromatin landscape of the human genomePreprint
Kim, S. H., Marinov, G. K., & Greenleaf, W. J. (2024) · Genome Research · doi:10.1101/gr.279621.124 · Cited by 4 · see citing works
Self-citationGenome-wide investigation of transcription factor footprints and dynamics using cFOOT-seq
Wang, H., Wu, A., Yang, M., Zhou, D., Chen, X., Shi, Z., Zhang, Y., Liu, Y.-X., Chen, K., Wang, X., et al. (2025) · Protein & Cell · doi:10.1093/procel/pwaf071 · Cited by 3 · see citing works
Somatic epimutations cap genetic determinism in the human diploid chromatin epigenome
Vollger, M. R., Swanson, E. G., Neph, S. J., Mallory, B. J., Dubocanin, D., Ho, C., Cheng, Y.-H. H., Ranchalis, J., Munson, K. M., Sedeño-Cortés, A. E., et al. (2026) · Cell · doi:10.1016/j.cell.2026.09.034 · Cited by 37 · see citing works
Zygotic activation of transposable elements during zebrafish early embryogenesis
Li, B., Li, T., Wang, D., Yang, Y., Tan, P., Wang, Y., Yang, Y.-G., Jia, S., & Au, K. F. (2025) · Nature Communications · doi:10.1038/s41467-025-58863-7 · Cited by 3 · see citing works
- Authors
- Shipony, Z., Marinov, G. K., Swaffer, M. P., Sinnott-Armstrong, N. A., Skotheim, J. M., Kundaje, A., & Greenleaf, W. J.
- Affiliations
- Stanford University (US)
- Chan Zuckerberg Biohub San Francisco (US)
- Corresponding author
- Greenleaf, William J.
- ORCID
- Venue
- Nature Methods
- Published
- 2020-02-10
- Volume
- 17
- Issue
- 3
- Pages
- 319-327
- ISSN
- 1548-7091 (ISSN-L)
- 1548-7105
- PMC
- PMC7968351
- OpenAlex
- W3004849394
- Cited by
- 160
- References
- 70
- Citations by year
- 2026: 20 · 2025: 23 · 2024: 34 · 2023: 29 · 2022: 18 · 2021: 24 · 2020: 10 · 2019: 2
- FWCI
- 6.73
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Licence
- Locations
- Nature Methods (publishedVersion)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by-nd)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Health & Human Services | NIH | National Human Genome Research Institute (P50HG007735, 1R01HG008140-01A1, 1UM1HG009442, 1UM1HG009436, 1U01HG009431)
- Division of Intramural Research, National Institute of Allergy and Infectious Diseases (U19AI057266)
- Human Frontier Science Program (RGY006S, LT 000835/2017)
- European Molecular Biology Organization (1119-2016)
- SU | School of Medicine, Stanford University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Keywords
- chromatin accessibility, open chromatin, single-molecule long-read sequencing, nanopore sequencing, DNA methylation, N6-methyladenosine, nucleosome positioning, transcription factor binding, distal regulatory elements
- MeSH terms
- Adenosine, Cell Line, Chromatin, Humans, Methylation, Methyltransferases, Nucleosomes, Promoter Regions, Genetic, Protein Binding, Saccharomyces cerevisiae, CpG Islands, DNA Methylation, Chromatin Immunoprecipitation, DNA Fragmentation, High-Throughput Nucleotide Sequencing
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review