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Cited by 78Signaling Pathways in DiseaseCalpain Protease Function and RegulationNuclear Receptors and Signaling

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Expression of the calmodulin-dependent protein phosphatase, calcineurin, in rat brain: developmental patterns and the role of nigrostriatal innervation

Polli, Joseph W., Billingsley, Melvin L., Kincaid, Randall L.·1991·Developmental Brain Research
DOI 10.1016/0165-3806(91)90071-p
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Abstract

The distribution of neurons expressing the calmodulin-dependent protein phosphatase, calcineurin (CN) was characterized in developing and adult rat brain using a combination of immunocytochemical, immunoblot and in situ hybridization approaches. Immunoblot analysis revealed a strong increase postnatally in CN protein expression. Four differently-charged isoforms of CN were observed in adult brain with apparent regional differences in isoform expression. Immunocytochemistry showed highest levels of CN in hippocampus, striatum, substantia nigra, amygdala and septal nuclei with immunoreactivity first appearing in striatum and septal nuclei, followed by hippocampus, neocortex and limbic structures. In situ hybridization demonstrated that mRNA for the catalytic subunit of CN was seen as early as postnatal day (PND) 1 in striatum, cortex and hippocampus. Since immunoreactivity was not detectable until day 4, this suggests that mRNA expression may precede that of protein by several days in these regions. Lesioning of developing and adult nigrostriatal dopamine neurons either with 6-hydroxydopamine or by surgical hemitransection had little effect on expression of CN, suggesting that CN expression is not influenced transsynaptically by dopamine. Collectively, these findings demonstrate that CN protein and mRNA expression are subject to regional and temporal control during brain development suggesting that specific synaptic connections may influence CN gene expression. However, in stri…

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  • Calcineurin A, and particularly its ␤ isoform expression in developing brain, is regulated posttranscriptionally, most likely at the translational level.

    “Calcineurin A, and particularly its ␤ isoform expression in developing brain is regulated posttranscriptionally, most likely at the translational level (Polli et al. 1991).”

    From A microRNA array reveals extensive regulation of microRNAs during brain development (2003) · cites this paper as Polli et al. 1991

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Metadata

Authors
Polli, J. W., Billingsley, M. L., & Kincaid, R. L.
Affiliations
  • Pennsylvania State University (US)
  • National Institutes of Health (US)
Corresponding author
Billingsley, Melvin L.
ORCID
Polli, Joseph W.
Venue
Developmental Brain Research
Publisher
Elsevier BV
Published
1991-11
Volume
63
Issue
1-2
Pages
105-119
ISSN
0165-3806 (ISSN-L)
DOI
10.1016/0165-3806(91)90071-p
OpenAlex
W2087353512
Cited by
78
References
1
Citations by year
2026: 1 · 2025: 1 · 2024: 1 · 2023: 2 · 2020: 1 · 2019: 1 · 2018: 3 · 2016: 4 · 2014: 3 · 2013: 1 · 2012: 4
FWCI
0.89
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Developmental Brain Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute on Aging (R01AG006377)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Calpain Protease Function and Regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Receptors and Signaling
Keywords
calcineurin, brain development, dopaminergic neurons, striatum, hippocampus, septal nuclei, substantia nigra, amygdala, regional gene expression, protein isoforms, mRNA expression, postnatal development, 6-hydroxydopamine lesion, synaptic connectivity
MeSH terms
Animals, Brain, Calmodulin-Binding Proteins, Cerebral Cortex, Corpus Striatum, Electrophoresis, Polyacrylamide Gel, Female, Immunoenzyme Techniques, Male, Nucleic Acid Hybridization, Phosphoprotein Phosphatases, Rats, Inbred Strains, Subcellular Fractions, Substantia Nigra, Immunoblotting, Oxidopamine, Calcineurin, Rats

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