The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Growth behavior of Chinese hamster ovary cells in a compact loop bioreactor. 2. Effects of medium components and waste products
Abstract
Effects of biochemical factors, i.e., medium components and metabolic byproducts, on growth of Chinese hamster ovary (CHO) cells were investigated. Glucose and ammonia were found to inhibit the growth. Kinetic analysis gave the inhibition constants, 0.14 g l-1 for ammonia and 5.0 g l-1 for glucose. Since glutamine was unstable and was a main source of ammonia, precise studies on glutamine degradation and ammonia formation process were done. By evaluating the spontaneous reactions, net glutamine utilization and net ammonia production by the cells could be estimated. It became evident that asparagine could support the growth of CHO cells as a stable substitute for glutamine. Then, a glucose fed-batch culture was grown on a glutamine free and asparagine supplemented medium. Because of (1) low glucose concentration, but (2) no glucose limitation and (3) low ammonia accumulation, the maximum total cell concentration reached 3.4 x 10(6) ml-1, which was 1.8 times greater than that in the control experiment (initial 1.15 g l-1 glucose and 0.29 g l-1 glutamine, and no glucose feed).
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“CHO cells are susceptible at concentrations as low as 4 mM (Kurano, Leist, Messi, Kurano, & Fiechter, 1990).”
From Impacts of intentional mycoplasma contamination on CHO cell bioreactor cultures (2019) · cites this paper as Kurano, Leist, Messi, Kurano, & Fiechter, 1990
Citations
Cites
The Theory of Splines and Their Applications
Ahlberg, J. H., Nilson, E. N., Walsh, J., & Bellman, R. (1967) · Mathematics in Science and Engineering/Mathematics in science and engineering · doi:10.1016/s0076-5392(08)x6115-6 · Cited by 1,855
A mathematical model for the continuous culture of microorganisms utilizing inhibitory substrates
Andrews, J. F. (1968) · Biotechnology and Bioengineering · doi:10.1002/bit.260100602 · Cited by 1,340
Fitting curves to data using nonlinear regression: a practical and nonmathematical review
Motulsky, H. J., & Ransnas, L. A. (1987) · The FASEB Journal · doi:10.1096/fasebj.1.5.3315805 · Cited by 1,327
A nonlinear regression program for small computers
Duggleby, R. G. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90104-4 · Cited by 564
Miller, W. M., Blanch, H. W., & Wilke, C. R. (1988) · Biotechnology and Bioengineering · doi:10.1002/bit.260320803 · Cited by 477
Glacken, M. W., Fleischaker, R. J., & Sinskey, A. J. (1986) · Biotechnology and Bioengineering · doi:10.1002/bit.260280912 · Cited by 347
Reciprocal regulation of glucose and glutamine utilization by cultured human diploid fibroblasts
Zielke, H. R., Ozand, P. T., Tildón, J. T., Sevdalian, D. A., & Cornblath, M. (1978) · Journal of Cellular Physiology · doi:10.1002/jcp.1040950106 · Cited by 273
Carbohydrate Metabolism in Cultured Cells
Morgan, M. J. (1986) · Springer eBooks · doi:10.1007/978-1-4684-7679-8 · Cited by 208
Spontaneous decomposition of glutamine in cell culture media
Tritsch, G. L., & Moore, G. E. (1962) · Experimental Cell Research · doi:10.1016/0014-4827(62)90290-2 · Cited by 199
Mathematical descriptions of hybridoma culture kinetics: I. Initial metabolic rates
Glacken, M. W., Adema, E., & Sinskey, A. J. (1988) · Biotechnology and Bioengineering · doi:10.1002/bit.260320412 · Cited by 186
Research works printed but not linked: 2
- Bergmeyer, 1985, Ammonia, Vol. 8, 454
- Lund, 1985, L-Glutamine and L-glutamate, vol. 8, 357
Cited by
Pereira, S., Kildegaard, H. F., & Andersen, M. R. (2018) · Biotechnology Journal · doi:10.1002/biot.201700499 · Cited by 214
Zhu, M. M., Goyal, A., Rank, D. L., Gupta, S. K., Boom, T. V., & Lee, S. S. (2008) · Biotechnology Progress · doi:10.1021/bp049815s · Cited by 199
Model-assisted Design of Experiments as a concept for knowledge-based bioprocess development
Möller, J., Kuchemüller, K. B., Steinmetz, T., Koopmann, K. S., & Pörtner, R. (2019) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-019-02089-7 · Cited by 100
Kirsch, B. J., Bennun, S. V., Mendez, A., Johnson, A. S., Wang, H., Qiu, H., Li, N., Lawrence, S. M., Bak, H., & Betenbaugh, M. J. (2022) · Biotechnology and Bioengineering · doi:10.1002/bit.27993 · Cited by 39
Impacts of intentional mycoplasma contamination on CHO cell bioreactor cultures
Fratz‐Berilla, E. J., Faison, T., Kohnhorst, C. L., Velugula‐Yellela, S. R., Powers, D. N., Brorson, K., & Agarabi, C. (2019) · Biotechnology and Bioengineering · doi:10.1002/bit.27161 · Cited by 14
Metadata
- Authors
- Kurano, N., Leist, C., Messi, F., Kurano, S., & Fiechter, A.
- Affiliations
- ETH Zurich (CH)
- Corresponding author
- Kurano, N.
- ORCID
- Fiechter, A.
- Venue
- Journal of Biotechnology
- Publisher
- Elsevier BV
- Published
- 1990-07
- Volume
- 15
- Issue
- 1-2
- Pages
- 113-128
- ISSN
- 0168-1656 (ISSN-L)
- OpenAlex
- W2030992249
- Cited by
- 104
- References
- 24
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 3 · 2021: 5 · 2020: 2 · 2019: 8 · 2018: 2 · 2017: 4 · 2016: 1 · 2015: 4 · 2014: 4 · 2013: 4 · 2012: 3
- FWCI
- 0.98
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Journal of Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Physical Sciences › Chemical Engineering › Fluid Flow and Transfer Processes › Fluid Dynamics and Mixing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- CHO cells, glucose inhibition, ammonia inhibition, asparagine, growth kinetics
- MeSH terms
- Ammonia, Animals, Asparagine, Cell Division, Cell Line, Cells, Cultured, Female, Glucose, Glutamine, Cricetinae, Kinetics, Lactates, Ovary, Oxidation-Reduction, Lactic Acid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Growth behavior of Chinese hamster ovary cells in a compact loop bioreactor. 2. Effects of medium components and waste products. https://app.cochat.ai/papers/10.1016/0168-1656(90)90055-g
- The paper
- Kurano, N., Leist, C., Messi, F., Kurano, S., & Fiechter, A. (1990). Growth behavior of Chinese hamster ovary cells in a compact loop bioreactor. 2. Effects of medium components and waste products. Journal of Biotechnology. https://doi.org/10.1016/0168-1656(90)90055-g
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
