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Cited by 86Antifungal resistance and susceptibilityYeasts and Rust Fungi StudiesFungal Infections and Studies

Strain delineation and antifungal susceptibilities of epidemiologically related and unrelated isolates of Candida lusitaniae

Pfaller, Michael A., Messer, S.A., Hollis, Richard J.·1994·Diagnostic Microbiology and Infectious Disease
DOI 10.1016/0732-8893(94)90106-6
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Abstract

Candida lusitaniae is an important nosocomial pathogen that may express resistance to one or more antifungal agents including amphotericin B. We investigated the genotypic diversity and antifungal susceptibility among 47 clinical isolates from 33 patients hospitalized in 12 different medical centers. Strain delineation was performed by restriction endonuclease analysis of genomic DNA (REAG) with the restriction enzyme Sfi I followed by pulsed-field gel electrophoresis and by electrophoretic karyotyping (EK). Antifungal susceptibility of the isolates to amphotericin B, 5-fluorocytosine (5FC), fluconazole, and itraconazole was determined using National Committee for Clinical Laboratory Standards standard methods. Minimum inhibitory concentration (MIC)90 values ranged from 0.5 micrograms/ml for itraconazole to 512 micrograms/ml for 5FC. In general, isolates from a given patient or epidemiologically related isolates from a nosocomial cluster were identical by molecular typing methods. Epidemiologically unrelated isolates were all distinctly different by both EK and REAG typing methods. Although elevated amphotericin B MICs ( > or = 2 micrograms/ml) were observed in only three isolates, extended incubation (72 h) revealed amphotericin B MICs of 2-16 micrograms/ml in a subset of isolates suggesting potential amphotericin B resistance. These data document the genetic diversity, nosocomial transmission, and antifungal susceptibility of clinical isolates of C. lusitaniae.

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  • Not all C. lusitaniae isolates are resistant, but therapeutic failure of amphotericin B is well documented.

    “Not all isolates are resistant [11,23,37], but therapeutic failure of amphotericin B is well documented [38].”

    From Guidelines for Treatment of Candidiasis (2004) · cites this paper as [23]

  • In virtually every U.S.-based survey, C. glabrata ranks second to C. albicans as a cause of BSI, accounting for 20% to 24% of all Candida BSIs.

    “and virtually every U.S.-based survey has shown C. glabrata to rank second to C. albicans as a cause of BSI, accounting for 20% to 24% of all Candida BSIs (Table 8) (57, 92, 113, 145, 146, 183, 188, 205, 207-209, 212, 216, 220, 224, 292).”

    From Epidemiology of Invasive Candidiasis: a Persistent Public Health Problem (2007) · cites this paper as [208]

  • Several studies have documented a significant trend toward an increased proportion of BSI due to C. glabrata with increasing patient age.

    “Several studies have now documented a significant trend toward an increased proportion of BSI due to C. glabrata with increasing patient age (Table 9 and Fig. 2) (23, 57, 90, 125, 145, 146, 188, 208, 209, 259).”

    From Epidemiology of Invasive Candidiasis: a Persistent Public Health Problem (2007) · cites this paper as [208]

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Authors
Pfaller, M. A., Messer, S. A., & Hollis, R. J.
Affiliations
University of Iowa (US)
ORCID
Venue
Diagnostic Microbiology and Infectious Disease
Publisher
Elsevier BV
Published
1994-11
Volume
20
Issue
3
Pages
127-133
ISSN
0732-8893 (ISSN-L)
DOI
10.1016/0732-8893(94)90106-6
OpenAlex
W2089087996
Cited by
86
References
26
Citations by year
2025: 1 · 2023: 1 · 2021: 1 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 2 · 2012: 2
FWCI
2.61
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Diagnostic Microbiology and Infectious Disease (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords
Candida lusitaniae, antifungal susceptibility, amphotericin B resistance, nosocomial transmission, genotypic diversity, molecular typing, electrophoretic karyotyping, restriction endonuclease analysis, 5-fluorocytosine, fluconazole, itraconazole, minimum inhibitory concentration, clinical isolates
MeSH terms
Antifungal Agents, Candida, Candidiasis, DNA, Fungal, Drug Resistance, Microbial, Humans, Karyotyping, Microbial Sensitivity Tests, Species Specificity, Genetic Variation, Deoxyribonucleases, Type II Site-Specific, Mycological Typing Techniques

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