Cited by 333Alzheimer's disease research and treatmentsUbiquitin and proteasome pathwaysParkinson's Disease Mechanisms and Treatments

Ubiquitin is conjugated with amino-terminally processed tau in paired helical filaments

Morishima-Kawashima, Maho, Hasegawa, Masato, Takio, Koji, Suzuki, Masami, Titani, Koiti, Ihara, Yasuo·1993·Neuron

Abstract

We have investigated ubiquitinated paired helical filaments, which produce a proteinaceous smear in SDS-polyacrylamide gel electrophoresis and immunoblotting. The smear consisted largely of the carboxy-terminal portion of tau and ubiquitin. The ubiquitin-targeted protein was identified as tau in paired helical filaments, and the conjugation sites were localized to the microtubule-binding region. Most ubiquitin in paired helical filaments occurred as a monoubiquitinated form, and only a small proportion of ubiquitin formed multiubiquitin chains. There was a ubiquitin-negative smear, in which tau was much less processed in the amino-terminal portion. This strongly suggests that the amino-terminal processing of tau in paired helical filaments precedes its ubiquitination.

Integrity signals

Registry

Crossref · 10.1016/0896-6273(93)90063-w

Cites

45 of 45 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Morishima-Kawashima, M., Hasegawa, M., Takio, K., Suzuki, M., Titani, K., & Ihara, Y.
Affiliations
  • The University of Tokyo (JP)
  • RIKEN (JP)
  • Fujita Health University (JP)
Venue
Neuron
Publisher
Elsevier BV
Published
1993-06
Volume
10
Issue
6
Pages
1151-1160
ISSN
0896-6273 (ISSN-L)
OpenAlex
W2012781495
Cited by
332
References
45
Citations by year
2026: 9 · 2025: 11 · 2024: 6 · 2023: 9 · 2022: 9 · 2021: 10 · 2020: 14 · 2019: 9 · 2018: 8 · 2017: 7 · 2016: 13 · 2015: 9 · 2014: 10 · 2013: 3 · 2012: 10
FWCI
10.43
Type
journal article
Language
en
Open access
closed
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education, Culture, Sports, Science and Technology
  • Ministry of Health and Welfare
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
  • Health Sciences › Medicine › Neurology › Parkinson's Disease Mechanisms and Treatments
Keywords
ubiquitin, tau protein, paired helical filaments, ubiquitination, microtubule-binding region, protein aggregation, Alzheimer's disease, ubiquitin chains, post-translational modification
MeSH terms
Amino Acid Sequence, Antibodies, Monoclonal, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Humans, Microtubules, Molecular Sequence Data, Molecular Weight, Peptide Fragments, Peptide Mapping, Endopeptidases, Protein Binding, Mass Spectrometry, Ubiquitins, Neurofibrillary Tangles, tau Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review