The role of Ca2+ channels in hippocampal mossy fiber synaptic transmission and long-term potentiation
Abstract
We have addressed the role of Ca2+ channels in mossy fiber synaptic transmission and long-term potentiation (LTP). Whereas the induction of mossy fiber LTP is entirely normal when synaptic transmission is blocked by the glutamate receptor antagonist kynurenate, LTP is blocked in the absence of extracellular Ca2+. These findings suggest that presynaptic Ca2+ entry is essential for mossy fiber LTP. Therefore, the role of different types of presynaptic Ca2+ channels in synaptic transmission and LTP was investigated. Mossy fiber responses were little affected by the L-type Ca2+ channel blocker nifedipine. They were blocked partially by omega-conotoxin-GVIA (N-type) and almost entirely by omega-agatoxin-IVA (P-type). None of these antagonists blocked mossy fiber LTP, nor was its expression associated with a change in sensitivity of synaptic transmission to either of the two toxins. These results, together with previous findings, suggest that the induction of mossy fiber LTP is critically dependent on the entry of Ca2+ into the presynaptic terminal to trigger a series of steps resulting in the long lasting enhancement of evoked glutamate release. Whereas P-type Ca2+ channels are of primary importance in mossy fiber synaptic transmission, both the induction and expression of mossy fiber LTP can occur in the absence of P-type (or N-type) Ca2+ channels.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
CaV2.1 is the major type of presynaptic Ca2+ channel in mossy fiber boutons.
“we targeted CaV2.1, the major type of presynaptic Ca2+ channel in MFBs [58,59]”
From Presynaptic cAMP-PKA-mediated potentiation induces reconfiguration of synaptic vesicle pools and channel-vesicle coupling at hippocampal mossy fiber boutons (2024) · cites this paper as [58]
Cited by
Sorted by citations
Nanodomain coupling between Ca2+ channels and sensors of exocytosis at fast mammalian synapses
Eggermann, E., Bucurenciu, I., Goswami, S. P., & Jonas, P. (2012) · Nature Reviews Neuroscience · doi:10.1038/nrn3125 · Cited by 500 · see citing works
Li, L., Bischofberger, J., & Jonas, P. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.1709-07.2007 · Cited by 206 · see citing works
Huang, Y.-Y., & Kandel, E. R. (1996) · Neuron · doi:10.1016/s0896-6273(00)80080-x · Cited by 199 · see citing works
Wang, H., Pineda, V. V., Chan, G. C. K., Wong, S. T., Muglia, L. J., & Storm, D. R. (2003) · The Journal of Neuroscience · doi:10.1523/jneurosci.23-30-09710.2003 · Cited by 120 · see citing works
Pelkey, K. A., Topolnik, L., Lacaille, J.-C., & McBain, C. J. (2006) · Neuron · doi:10.1016/j.neuron.2006.08.032 · Cited by 115 · see citing works
Gasparini, S., Kasyanov, A. M., Pietrobon, D., Voronin, L. L., & Cherubini, E. (2001) · The Journal of Neuroscience · doi:10.1523/jneurosci.21-22-08715.2001 · Cited by 114 · see citing works
Pelkey, K. A., Topolnik, L., Yuan, X.-Q., Lacaille, J.-C., & McBain, C. J. (2008) · Neuron · doi:10.1016/j.neuron.2008.11.018 · Cited by 69 · see citing works
Dopamine selectively potentiates hippocampal mossy fiber to CA3 synaptic transmission
Kobayashi, K., & Suzuki, H. (2007) · Neuropharmacology · doi:10.1016/j.neuropharm.2006.08.026 · Cited by 63 · see citing works
Pelkey, K. A., & McBain, C. J. (2008) · The Journal of Physiology · doi:10.1113/jphysiol.2007.148635 · Cited by 58 · see citing works
Definition of a Bidirectional Activity-Dependent Pathway Involving BDNF and Narp
Mariga, A., Glaser, J., Mathias, L., Xu, D., Xiao, M., Worley, P., Ninan, I., & Chao, M. V. (2015) · Cell Reports · doi:10.1016/j.celrep.2015.10.064 · Cited by 45 · see citing works
cAMP-Dependent Synaptic Plasticity at the Hippocampal Mossy Fiber Terminal
Shahoha, M., Cohen, R., Ben-Simon, Y., & Ashery, U. (2022) · Frontiers in Synaptic Neuroscience · doi:10.3389/fnsyn.2022.861215 · Cited by 46 · see citing works
Structure, biophysics, and circuit function of a “giant” cortical presynaptic terminal
Vandael, D., & Jonas, P. (2024) · Science · doi:10.1126/science.adg6757 · Cited by 45 · see citing works
Fukaya, R., Maglione, M., Sigrist, S. J., & Sakaba, T. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2016754118 · Cited by 42 · see citing works
Martinello, K., Giacalone, E., Migliore, M., Brown, D. A., & Shah, M. M. (2019) · Communications Biology · doi:10.1038/s42003-019-0408-4 · Cited by 32 · see citing works
Kim, O., Okamoto, Y., Kaufmann, W. A., Brose, N., Shigemoto, R., & Jonas, P. (2024) · PLOS Biology · doi:10.1371/journal.pbio.3002879 · Cited by 15 · see citing works
Calcium activated adenylyl cyclase AC8 but not AC1 is required for prolonged behavioral anxiety
Bernabucci, M., & Zhuo, M. (2016) · Molecular Brain · doi:10.1186/s13041-016-0239-x · Cited by 11 · see citing works
- Authors
- Castillo, P. E., Weisskopf, M. G., & Nicoll, R. A.
- Affiliations
- University of California, San Francisco (US)
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 1994-02
- Volume
- 12
- Issue
- 2
- Pages
- 261-269
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W1969167894
- Cited by
- 298
- References
- 0
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 4 · 2023: 7 · 2022: 6 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 4 · 2017: 3 · 2016: 7 · 2015: 2 · 2014: 4 · 2013: 8 · 2012: 4
- FWCI
- 8.26
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Neuroscience › Cognitive Neuroscience › Memory and Neural Mechanisms
- Keywords
- calcium channels, mossy fiber synapses, long-term potentiation, presynaptic calcium entry, P-type calcium channels, N-type calcium channels, omega-conotoxin GVIA, omega-agatoxin IVA, nifedipine, kynurenic acid, glutamate release, hippocampus, synaptic transmission
- MeSH terms
- Animals, Calcium, Calcium Channel Blockers, Guinea Pigs, Hippocampus, Nerve Fibers, Synaptic Transmission, Calcium Channels, Long-Term Potentiation, In Vitro Techniques
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
