The DOI matches its OpenAlex record; 7 of the 7 references it cites were found in a registry or an index.
Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction
Open accessAbstract
Primers to amplify the genes encoding the virulence factors of uropathogenic Escherichia coli, such as pilus associated with pyelonephritis (pap), haemolysin (hly), aerobactin (aer) and cytotoxic necrotizing factor 1 (cnf1) genes, were designed. The above primers along with previously reported primers for S fimbriae (sfa) and afimbrial adhesin I (afaI) genes were combined to develop a multiplex polymerase chain reaction (PCR) for detection of the respective virulence factors and for the identification of uropathogenic E. coli. The multiplex PCR to detect pap, sfa, afaI, hly, aer and cnf1 genes was highly specific and the sensitivity was found to be about 5 x 10(3) colony forming units of E. coli per ml. A total of 194 E. coli strains isolated from patients with simple acute cystitis were examined by the multiplex PCR and the results were in complete agreement with that obtained by DNA colony hybridization test. The multiplex PCR developed was, therefore, concluded to be a useful, sensitive and rapid assay system to identify uropathogenic E. coli.
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Cites
Nucleotide sequence of an Escherichia coli chromosomal hemolysin
Felmlee, T., Pellett, S., & Welch, R. A. (1985) · Journal of Bacteriology · doi:10.1128/jb.163.1.94-105.1985 · Cited by 545
Detection of Enterotoxigenic Escherichia coli by DNA Colony Hybridization
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Le Bouguenec, C., Archambaud, M., & Labigne, A. (1992) · Journal of Clinical Microbiology · doi:10.1128/jcm.30.5.1189-1193.1992 · Cited by 341
Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12
de Lorenzo, V, Bindereif, A., Paw, B. H., & Neilands, J. B. (1986) · Journal of Bacteriology · doi:10.1128/jb.165.2.570-578.1986 · Cited by 225
Falbo, V., Pace, T., Picci, L., Pizzi, E., & Caprioli, A. (1993) · Infection and Immunity · doi:10.1128/iai.61.11.4909-4914.1993 · Cited by 133
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Cited by
Johnson, J. R., & Stell, A. L. (2000) · The Journal of Infectious Diseases · doi:10.1086/315217 · Cited by 1,269
Nowrouzian, F., Hesselmar, B., Saalman, R., Strannegård, I.-L., Åberg, N., Wold, A. E., & Adlerberth, I. (2003) · Pediatric Research · doi:10.1203/01.pdr.0000069843.20655.ee · Cited by 176
Virulence factors of Escherichia coli isolated from bovine clinical mastitis
Kaipainen, T., Pohjanvirta, T., Shpigel, N. Y., Shwimmer, A., Pyörälä, S., & Pelkonen, S. (2002) · Veterinary Microbiology · doi:10.1016/s0378-1135(01)00483-7 · Cited by 83
Ahmed, H. A., Elsohaby, I., Elamin, A. M., El-Ghafar, A. E. A., Elsaid, G. A., Elbarbary, M., Mohsen, R. A., El Feky, T. M., & El Bayomi, R. M. (2023) · BMC Microbiology · doi:10.1186/s12866-023-02948-0 · Cited by 25
Metadata
- Authors
- Yamamoto, S., Terai, A., Yuri, K., Kurazono, H., Takeda, Y., & Yoshida, O.
- Venue
- FEMS Immunology & Medical Microbiology
- Publisher
- Oxford University Press
- Published
- 1995-10-01
- Volume
- 12
- Issue
- 2
- Pages
- 85-90
- ISSN
- 0928-8244 (ISSN-L)
- OpenAlex
- W2074530999
- Cited by
- 353
- References
- 7
- FWCI
- 1.50
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Yamamoto, S., Terai, A., Yuri, K., Kurazono, H., Takeda, Y., & Yoshida, O. (1995). Detection of urovirulence factors in Escherichia coli by multiplex polymerase chain reaction. FEMS Immunology & Medical Microbiology. https://doi.org/10.1016/0928-8244(95)00053-a
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
