An ultrasensitive fluorescent assay for the in vivo quantification of superoxide radical in organisms
Abstract
Superoxide radical is a very important parameter of oxidative stress involved in a variety of biological phenomena; therefore, its in vivo study is of utmost significance. However, its accurate detection is a challenge due to its short lifetime and its very low physiological concentration. All current assays are qualitative and nonspecific, and at best they are performed in vitro. The current dihydroethidine-based assay overcomes all these problems and introduces the following novelties. First, it measures the in vivo superoxide production in animals, plants, and microorganisms. Second, it is ultrasensitive and very simple in that it can measure superoxide radical as low as 1.5 pmol in biological samples as low as 5 mg. Third, the very high sensitivity of the assay renders possible, for the first time, the measurement of the actual rate of formation of superoxide radical under physiological and simulated nonphysiological conditions.
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Superoxide radicals in amounts as low as 1.5 pmol in 5 mg of biological samples can be quantified fluorometrically with hydroethidine.
“The production of superoxide radicals in amounts as low as 1.5 pmol in 5 mg of biological samples can be quantified fluorometrically with this probe [158], Figure 62.”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [158]
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Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species
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Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity
Andrés, C. M. C., Pérez de la Lastra, J. M., Andrés Juan, C., Plou, F. J., & Pérez-Lebeña, E. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24031841 · Cited by 342 · see citing works
- Authors
- Georgiou, C. D., Papapostolou, I., Patsoukis, N., Tsegenidis, T., & Sideris, T.
- Affiliations
- University of Patras (GR)
- Corresponding author
- Georgiou, Christos D.
- Venue
- Analytical Biochemistry
- Publisher
- Elsevier BV
- Published
- 2005-12
- Volume
- 347
- Issue
- 1
- Pages
- 144-151
- ISSN
- 0003-2697 (ISSN-L)
- OpenAlex
- W1983050274
- Cited by
- 70
- References
- 31
- Citations by year
- 2026: 1 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 4 · 2016: 1 · 2015: 5 · 2014: 4 · 2013: 5 · 2012: 5
- FWCI
- 1.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Analytical Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Funders
- European Social Fund
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
- Keywords
- superoxide, fluorometric assay, oxidative stress, reactive oxygen species detection, ultrasensitive assay
- MeSH terms
- Animals, Endothelium, Vascular, Humans, Plants, Saccharomyces cerevisiae, Spectrometry, Fluorescence, Superoxides
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