Cited by 70Redox biology and oxidative stressAntioxidant Activity and Oxidative StressMolecular Sensors and Ion Detection

An ultrasensitive fluorescent assay for the in vivo quantification of superoxide radical in organisms

Georgiou, Christos D., Papapostolou, Ioannis, Patsoukis, Nikolaos, Tsegenidis, Theodore, Sideris, Theodore·2005·Analytical Biochemistry

Abstract

Superoxide radical is a very important parameter of oxidative stress involved in a variety of biological phenomena; therefore, its in vivo study is of utmost significance. However, its accurate detection is a challenge due to its short lifetime and its very low physiological concentration. All current assays are qualitative and nonspecific, and at best they are performed in vitro. The current dihydroethidine-based assay overcomes all these problems and introduces the following novelties. First, it measures the in vivo superoxide production in animals, plants, and microorganisms. Second, it is ultrasensitive and very simple in that it can measure superoxide radical as low as 1.5 pmol in biological samples as low as 5 mg. Third, the very high sensitivity of the assay renders possible, for the first time, the measurement of the actual rate of formation of superoxide radical under physiological and simulated nonphysiological conditions.

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Crossref · 10.1016/j.ab.2005.09.013

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  • Superoxide radicals in amounts as low as 1.5 pmol in 5 mg of biological samples can be quantified fluorometrically with hydroethidine.

    “The production of superoxide radicals in amounts as low as 1.5 pmol in 5 mg of biological samples can be quantified fluorometrically with this probe [158], Figure 62.”

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Authors
Georgiou, C. D., Papapostolou, I., Patsoukis, N., Tsegenidis, T., & Sideris, T.
Affiliations
University of Patras (GR)
Corresponding author
Georgiou, Christos D.
Venue
Analytical Biochemistry
Publisher
Elsevier BV
Published
2005-12
Volume
347
Issue
1
Pages
144-151
ISSN
0003-2697 (ISSN-L)
OpenAlex
W1983050274
Cited by
70
References
31
Citations by year
2026: 1 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 2 · 2017: 4 · 2016: 1 · 2015: 5 · 2014: 4 · 2013: 5 · 2012: 5
FWCI
1.88
Type
journal article
Language
en
Open access
closed
Locations
  • Analytical Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
European Social Fund
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Physical Sciences › Chemistry › Spectroscopy › Molecular Sensors and Ion Detection
Keywords
superoxide, fluorometric assay, oxidative stress, reactive oxygen species detection, ultrasensitive assay
MeSH terms
Animals, Endothelium, Vascular, Humans, Plants, Saccharomyces cerevisiae, Spectrometry, Fluorescence, Superoxides

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