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Microfluidics for cell-based high throughput screening platforms—A review
Open accessAbstract
In the last decades, the basic techniques of microfluidics for the study of cells such as cell culture, cell separation, and cell lysis, have been well developed. Based on cell handling techniques, microfluidics has been widely applied in the field of PCR (Polymerase Chain Reaction), immunoassays, organ-on-chip, stem cell research, and analysis and identification of circulating tumor cells. As a major step in drug discovery, high-throughput screening allows rapid analysis of thousands of chemical, biochemical, genetic or pharmacological tests in parallel. In this review, we summarize the application of microfluidics in cell-based high throughput screening. The screening methods mentioned in this paper include approaches using the perfusion flow mode, the droplet mode, and the microarray mode. We also discuss the future development of microfluidic based high throughput screening platform for drug discovery.
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Metadata
- Authors
- Du, G., Fang, Q., & den Toonder, J. M. J.
- Affiliations
- Zhejiang University (CN)
- Corresponding author
- Fang, Qun
- ORCID
- Fang, Qun
- Venue
- Analytica Chimica Acta
- Publisher
- Elsevier BV
- Published
- 2016-01
- Volume
- 903
- Pages
- 36-50
- ISSN
- 0003-2670 (ISSN-L)
- OpenAlex
- W2175894202
- Semantic Scholar
- 19c0d49efc26c0f85b416a428d8d90b8d69be2e7
- Cited by
- 276
- References
- 81
- Citations by year
- 2026: 11 · 2025: 14 · 2024: 29 · 2023: 21 · 2022: 31 · 2021: 36 · 2020: 37 · 2019: 31 · 2018: 31 · 2017: 27 · 2016: 8
- FWCI
- 1.70
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Analytica Chimica Acta (publishedVersion)
- PubMed (publishedVersion)
- TU/e Research Portal (submittedVersion, other-oa)
- TU/e Research Portal (submittedVersion)
- Funders
- National Natural Science Foundation of China (20825517, 21227007, 21435004)
- Major National Science and Technology Programs (2013ZX09507005)
- Philips Research
- Topics
- Physical Sciences › Chemical Engineering › Fluid Flow and Transfer Processes › Innovative Microfluidic and Catalytic Techniques Innovation
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Bio-sensing Technologies
- Keywords
- microfluidics, cell-based high-throughput screening, drug discovery, droplet microfluidics, microarray, organ-on-a-chip, cell isolation, circulating tumor cells, stem cell research, immunoassay, PCR
- MeSH terms
- Microfluidics, High-Throughput Screening Assays
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