Cited by 430Bone Tissue Engineering MaterialsElectrospun Nanofibers in Biomedical Applicationsbiodegradable polymer synthesis and properties

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Electrospun nanostructured scaffolds for bone tissue engineering

Prabhakaran, Molamma P., Venugopal, J., Ramakrishna, S.·2009·Acta Biomaterialia

DOI 10.1016/j.actbio.2009.05.007

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Abstract

The current challenge in bone tissue engineering is to fabricate a bioartificial bone graft mimicking the extracellular matrix (ECM) with effective bone mineralization, resulting in the regeneration of fractured or diseased bones. Biocomposite polymeric nanofibers containing nanohydroxyapatite (HA) fabricated by electrospinning could be promising scaffolds for bone tissue engineering. Nanofibrous scaffolds of poly-l-lactide (PLLA, 860+/-110 nm), PLLA/HA (845+/-140 nm) and PLLA/collagen/HA (310+/-125 nm) were fabricated, and the morphology, chemical and mechanical characterization of the nanofibers were evaluated using scanning electron microscopy, Fourier transform infrared spectroscopy and tensile testing, respectively. The in vitro biocompatibility of different nanofibrous scaffolds was also assessed by growing human fetal osteoblasts (hFOB), and investigating the proliferation, alkaline phosphatase activity (ALP) and mineralization of cells on different nanofibrous scaffolds. Osteoblasts were found to adhere and grow actively on PLLA/collagen/HA nanofibers with enhanced mineral deposition of 57% higher than the PLLA/HA nanofibers. The synergistic effect of the presence of an ECM protein, collagen and HA in PLLA/collagen/HA nanofibers provided cell recognition sites together with apatite for cell proliferation and osteoconduction necessary for mineralization and bone formation. The results of our study showed that the biocomposite PLLA/collagen/HA nanofibrous scaffold could be a potential substrate for the proliferation and mineralization of osteoblasts, enhancing bone regeneration.

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Biological evaluation and osteogenic potential of polyhydroxybutyrate-keratin/Al2O3 electrospun nanocomposite scaffold: A novel bone regeneration construct
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The potential of biomimetic nanofibrous electrospun scaffold comprising dual component for bone tissue engineering
Jaganathan, S. K., Mani, M. P., Nageswaran, G., Pandiyaraj, K. N., & Manikandan, A. (2019) · International Journal of Polymer Analysis and Characterization · doi:10.1080/1023666x.2018.1564127 · Cited by 17
Collagen Self-assembly: Biophysics and Biosignaling for Advanced Tissue Generation
Sohutskay, D. O., Puls, T. J., & Voytik‐Harbin, S. L. (2019) · Studies in mechanobiology, tissue engineering and biomaterials · doi:10.1007/978-3-030-20182-1_7 · Cited by 16
In vitro blood compatibility and bone mineralization aspects of polymeric scaffold laden with essential oil and metallic particles for bone tissue engineering
Zhang, Z., Yu, Z., Zhang, L., Mani, M. P., & Jaganathan, S. K. (2019) · International Journal of Polymer Analysis and Characterization · doi:10.1080/1023666x.2019.1611029 · Cited by 17
“The addition of LA and CoNO3 into PU matrix resulted in the smaller fiber diameter which might be suitable for the growth of osteoblast cells.([18,19]) Further, EDX was performed to confirm the presence of cobalt in the polyurethane matrix.”
Background
Bone tissue regeneration
Bassi, A. L., Gough, J. E., Zakikhani, M., & Downes, S. (2011) · Elsevier eBooks · doi:10.1533/9780857092915.2.93 · Cited by 19
3D printed high-precision porous scaffolds prepared by fused deposition modeling induce macrophage polarization to promote bone regeneration
Wang, X., Fu, X., Luo, D., Hou, R., Li, P.-W., Chen, Y., Zhang, X., Meng, X., Yue, Y., & Liu, J. (2024) · Biomedical Materials · doi:10.1088/1748-605x/ad2ed0 · Cited by 16
“This method stands in contrast to conventional manufacturing methods such as freeze drying [21, 22] and electrostatic spinning [23–25].”
Method
Magnesium glassy alloy laminated nanofibrous polymer as biodegradable scaffolds
Abisegapriyan, K. S., Rajeshwari, A., Kundu, S., & Subramanian, B. (2018) · Journal of Non-Crystalline Solids · doi:10.1016/j.jnoncrysol.2018.09.011 · Cited by 16
Surface modification of titanium implants via PLLA/HA fibrous composite coating to improve piezoelectric properties
Fotoukian, H., Nazarpak, M. H., Solouk, A., Bagheri, F., Akbari, S., & Nouri, A. (2024) · Surfaces and Interfaces · doi:10.1016/j.surfin.2024.104501 · Cited by 15
Biodegradable and bioactive polymer/inorganic phase nanocomposites for bone tissue engineering (BTE)
Miguez-Pacheco, V., Misra, S. K., & Boccaccini, A. R. (2014) · Elsevier eBooks · doi:10.1533/9780857097163.1.115 · Cited by 15
Compression of Multilayered Composite Electrospun Scaffolds: A Novel Strategy to Rapidly Enhance Mechanical Properties and Three Dimensionality of Bone Scaffolds
Madurantakam, P. A., Rodriguez, I. A., Garg, K., McCool, J. M., Moon, P. C., & Bowlin, G. L. (2013) · Advances in Materials Science and Engineering · doi:10.1155/2013/561273 · Cited by 14
Electrospun materials for bone and tendon/ligament tissue engineering
Bölgen, N. (2017) · Elsevier eBooks · doi:10.1016/b978-0-08-101022-8.00004-1 · Cited by 14
Bioinspired Structures and Design
Askarinejad, S., & Rahbar, N. (2020) · Cambridge University Press eBooks · doi:10.1017/9781139058995 · Cited by 15
Evaluation of the Osteoblast Behavior to PGA Textile Functionalized with RGD as a Scaffold for Bone Regeneration
Ortiz, M., Escobar‐García, D. M., Álvarez-Pérez, M. A., Pozos‐Guillén, A., Grandfils, C., & Flores, H. (2017) · Journal of Nanomaterials · doi:10.1155/2017/4852190 · Cited by 14
Mineralization and Characterization of Composite Lyophilized Gelatin Sponges Intended for Early Bone Regeneration
Rodriguez, I., Saxena, G., Sell, S. A., & Bowlin, G. L. (2014) · Bioengineering · doi:10.3390/bioengineering1010062 · Cited by 14
Triple-gene deletion for osteocalcin significantly impairs the alignment of hydroxyapatite crystals and collagen in mice
Xu, Z., Yang, C., Wu, F., Tan, X., Guo, Y., Zhang, H., Wang, H., Sui, X., Xu, Z., Zhao, M., et al. (2023) · Frontiers in Physiology · doi:10.3389/fphys.2023.1136561 · Cited by 13
“Cross-linking is crucial for the stability and mineralization of collagen (Hakimimehr et al., 2005; Prabhakaran et al., 2009; Terajima et al., 2014).”
A novel method for fabricating continuous polymer nanofibers
Du, J. H., Liu, D., Chen, S., Wan, D., & Pu, H. (2016) · Polymer · doi:10.1016/j.polymer.2016.09.018 · Cited by 15
The effect of medium composition on deposition of collagen type 1 and expression of osteogenic genes in mesenchymal stem cells derived from human adipose tissue and bone marrow
Szöke, K., Daňková, J., Buzgo, M., Amler, E., Brinchmann, J. E., & Østrup, E. (2016) · Process Biochemistry · doi:10.1016/j.procbio.2016.10.011 · Cited by 12
Research trends of piezoelectric biomaterials in osteochondral tissue engineering
Liu, Y., & Lu, J. (2024) · Materials Today Communications · doi:10.1016/j.mtcomm.2024.110264 · Cited by 12
Biomimetic Nanofibers for Musculoskeletal Tissue Engineering
Mohan, N., & Detamore, M. S. (2015) · Elsevier eBooks · doi:10.1016/b978-0-323-32889-0.00004-2 · Cited by 12
Electrospun hydrogels composites for bone tissue engineering
Chee, B. S., de Lima, G. G., Devine, D. M., & Nugent, M. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-813740-6.00003-x · Cited by 12
Homogeneous organic/inorganic hybrid scaffolds with high osteoinductive activity for bone tissue engineering
Liu, L., Li, C., Jiao, Y., Jiang, G., Mao, J., Wang, F., & Wang, L. (2020) · Polymer Testing · doi:10.1016/j.polymertesting.2020.106798 · Cited by 12
Cytotoxicity Study of UV-Laser-Irradiated PLLA Surfaces Subjected to Bio-Ceramisation: A New Way towards Implant Surface Modification
Szustakiewicz, K., Kryszak, B., Dzienny, P., Poźniak, B., Tikhomirov, M., Hoppe, V., Szymczyk‐Ziółkowska, P., Tylus, W., Grzymajło, M., Gadomska‐Gajadhur, A., et al. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22168436 · Cited by 12
“The PLLA/apatite materials can be obtained using the technique of extrusion [8–10], but also using solvent techniques including thermally induced phase separation [11–14], electrospinning [15,16] and solvent casting [17].”
Method
Influence of Nonsulfated Polysaccharides on the Properties of Electrospun Poly(lactic-co-glycolic acid) Fibers
Azeem, A., Marani, L., Fuller, K., Spanoudes, K., Pandit, A. S., & Zeugolis, D. I. (2016) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.6b00206 · Cited by 11
Development and Characterization of Multifunctional Wound Dressing with the Property of Anti-bacteria and Angiogenesis
He, X. J., Zhou, M., Chen, X., Wang, J., Zhao, X., Zhu, Y., & Liu, T. (2022) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-022-09914-8 · Cited by 11
“But, PLA-based nanofibrous scaffolds in wound healing showed lower bioactivity, and the acidic degradation product of PLA (lactic acid) could induce severe inflammation in vivo [6, 7].”
Background
Highly effective electrospun polycaprolactone/ layered double hydroxide nanofibrous scaffold for bone tissue engineering
Dehkordi, A. N., Shafiei, S. S., Chehelgerdi, M., Sabouni, F., Sharifi, E., Makvandi, P., & Nasrollahi, N. (2022) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2022.103827 · Cited by 11
Curcumin nanoparticles impregnated collagen/demineralized bone matrix/olive leaves extract biocomposites as a potential bone implant: Preparation, characterization, and biocompatibility
Senthil, R., Sumathi, V., Sivakumar, P. M., Kavukcu, S. B., Thangavelu, L. P., & Ahmed, A. (2023) · MRS Communications · doi:10.1557/s43579-023-00324-7 · Cited by 11
“[23] Prabhakaran et al.[24] discovered biocomposite PLLA/collagen/HA scaffolds to be promising substrates for mineralization and osteoblast proliferation, resulting in improved bone regeneration.”
Background
Development and optimisation of hydroxyapatite-polyethylene glycol diacrylate hydrogel inks for 3D printing of bone tissue engineered scaffolds
Rajabi, M., Cabral, J. D., Saunderson, S. C., Gould, M. L., & Ali, A. (2023) · Biomedical Materials · doi:10.1088/1748-605x/acf90a · Cited by 12
Characterization of maghemite (γ-Fe2O3)-loaded poly-l-lactic acid/thermoplastic polyurethane electrospun mats for soft tissue engineering
Fallahiarezoudar, E., Ahmadipourroudposht, M., Idris, A., Yusof, N. M., Marvibaigi, M., & Irfan, M. (2016) · Journal of Materials Science · doi:10.1007/s10853-016-0087-1 · Cited by 11
“[40] reported the application of PLLA/chitosan for bone scaffold; Amarjargal et al.”
Method
Nanofibers and Nanoparticles in Biomedical Applications
Baptista, A. C., Soares, P. I. P., Ferreira, I., & Borges, J. P. (2013) · CRC Press eBooks · doi:10.1201/b15403-5 · Cited by 11
A quaternary composite fiber membrane for guided tissue regeneration
Li, H., Song, P., Qiao, T., Cui, Q., Song, X., & Zhang, B. (2015) · Polymers for Advanced Technologies · doi:10.1002/pat.3616 · Cited by 10
Osteogenic induction of human mesenchymal stem cells in multilayered electrospun scaffolds at different flow rates and configurations in a perfusion bioreactor
Yaghoobi, M., Hashemi‐Najafabadi, S., Soleimani, M., & Vasheghani‐Farahani, E. (2019) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2019.03.015 · Cited by 10
Sustained release of strontium (Sr 2+ ) from polycaprolactone/poly (D,L-lactide-co-glycolide)-polyvinyl alcohol coaxial nanofibers enhances osteoblastic differentiation
Chen, L., Mazeh, H., Guardia, A., Song, W., Begeman, P. C., Markel, D. C., & Ren, W. (2019) · Journal of Biomaterials Applications · doi:10.1177/0885328219858736 · Cited by 10
Fabrication of Mesoporous Silica Nanoparticle–Incorporated Coaxial Nanofiber for Evaluating the In Vitro Osteogenic Potential
Pathmanapan, S., Sekar, M., Pandurangan, A. K., & Anandasadagopan, S. K. (2021) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-021-03741-3 · Cited by 10
“Then, the ALP produced in the culture will hydrolyze the phosphate esters and enhance the phosphate concentration in the culture, which will further enhance the mineralization of the extracellular matrix [38].”
BackgroundInfluential
Benefits of Renewable Hydrogels over Acrylate- and Acrylamide-Based Hydrogels
Mallik, A. K., Shahruzzaman, M., Sakib, M. N., Zaman, A., Rahman, M. S., Islam, M. M., Islam, M. S., Haque, P., & Rahman, M. M. (2019) · Polymers and polymeric composites · doi:10.1007/978-3-319-77830-3_10 · Cited by 10
Physical and biological activities of newly designed, macro-pore-structure-controlled 3D fibrous poly(ε-caprolactone)/hydroxyapatite composite scaffolds
Kim, M., & Kim, G. (2015) · RSC Advances · doi:10.1039/c5ra00915d · Cited by 10
Silk fibroin-copper nanoparticles conglomerated polyurethane fibers incorporating calcium carbonate for enhanced fluid retention, antibacterial efficacy and promotion of cell growth
Khan, R. S., Qureashi, A., Rafiq, M., Rather, A. H., Reshi, M. M., Qurashi, A., Tripathi, R. M., & Sheikh, F. A. (2024) · Journal of Drug Delivery Science and Technology · doi:10.1016/j.jddst.2024.105464 · Cited by 9

Authors: Prabhakaran, M. P., Venugopal, J., & Ramakrishna, S.
Affiliations: National University of Singapore (SG)
Corresponding author: Prabhakaran, Molamma P.
ORCID: Prabhakaran, Molamma P.
Venugopal, J.
Ramakrishna, S.
Venue: Acta Biomaterialia
Publisher: Elsevier BV
Published: 2009-10
Volume: 5
Issue: 8
Pages: 2884-2893
ISSN: 1742-7061 (ISSN-L)
DOI: 10.1016/j.actbio.2009.05.007
OpenAlex: W2070366856
Cited by: 430
References: 35
Citations by year: 2026: 10 · 2025: 11 · 2024: 23 · 2023: 16 · 2022: 24 · 2021: 17 · 2020: 42 · 2019: 31 · 2018: 23 · 2017: 36 · 2016: 34 · 2015: 25 · 2014: 34 · 2013: 30 · 2012: 30
FWCI: 14.82
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Acta Biomaterialia (publishedVersion)
PubMed (publishedVersion)
National University of Singapore (submittedVersion)
Funders: National Medical Research Council
Topics: Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
Keywords: electrospun nanofibers, bone tissue engineering, poly(L-lactic acid), hydroxyapatite nanoparticles, collagen, biocomposite scaffolds, osteoblast mineralization, alkaline phosphatase activity, osteoblast proliferation, bone regeneration, extracellular matrix mimicry, osteoconduction
MeSH terms: Absorption, Cell Line, Electrochemistry, Extracellular Matrix, Humans, Materials Testing, Osteoblasts, Osteogenesis, Particle Size, Polyesters, Polymers, Rotation, Surface Properties, Porosity, Bone Substitutes, Cell Culture Techniques, Lactic Acid, Tissue Engineering, Biomimetic Materials, Cell Enlargement, Nanostructures

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Electrospun nanostructured scaffolds for bone tissue engineering

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