Production and characterization of engineered alginate-based microparticles containing ECM powder for cell/tissue engineering applications
Abstract
A method for the production of engineered alginate-based microparticles, containing extracellular matrix and neonatal porcine Sertoli cells (SCs), is described. As a source for extracellular matrix, a powder form of isolated and purified urinary bladder matrix (UBM) was employed. We demonstrated that the incorporation of UBM does not significantly alter the morphological and dimensional characteristics of the microparticles. The alginate microparticles were used for SC encapsulation as an immunoprotective barrier for transplant purposes, while the co-entrapped UBM promoted retention of cell viability and function. These engineered microparticles could represent a novel approach to enhancing immunological acceptance and increasing the functional life-span of the entrapped cells for cell/tissue engineering applications. In this respect, it is noteworthy that isolated neonatal porcine SCs, administered alone in highly biocompatible microparticles, led to diabetes prevention and reversion in nonobese diabetic (NOD) mice.
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Such 'papers' have been demonstrated to support adhesion, viability, and proliferation of human MSCs across diverse tissues.
“Such “papers” have been demonstrated to support adhesion, viability, and proliferation of human MSCs across diverse tissues.[ 150 ]”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [150]
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- Authors
- Mazzitelli, S., Luca, G., Mancuso, F., Calvitti, M., Calafiore, R., Nastruzzi, C., Johnson, S., & Badylak, S. F.
- Affiliations
- University of Perugia (IT)
- University of Pittsburgh (US)
- Corresponding author
- Nastruzzi, Claudio
- ORCID
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2011-03
- Volume
- 7
- Issue
- 3
- Pages
- 1050-1062
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W2081809504
- Cited by
- 41
- References
- 33
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 2 · 2017: 5 · 2016: 6 · 2015: 3 · 2014: 4 · 2013: 2 · 2012: 5
- FWCI
- 3.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- Institutional Research Information System University of Ferrara (University of Ferrara) (submittedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Keywords
- alginate microparticles, extracellular matrix, urinary bladder matrix, Sertoli cells, cell encapsulation, cell transplantation, cell viability, diabetes prevention, NOD mice, tissue engineering scaffolds
- MeSH terms
- Alginates, Animals, Base Sequence, Urinary Bladder, Extracellular Matrix, Hexuronic Acids, Male, Microspheres, Powders, Swine, DNA Primers, Reverse Transcriptase Polymerase Chain Reaction, Glucuronic Acid, Tissue Engineering
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