3D bioprinting of urethra with PCL/PLCL blend and dual autologous cells in fibrin hydrogel: An in vitro evaluation of biomimetic mechanical property and cell growth environment
Abstract
Objective Urethral stricture is a common condition seen after urethral injury. The currently available treatments are inadequate and there is a scarcity of substitute materials used for treatment of urethral stricture. The traditional tissue engineering of urethra involves scaffold design, fabrication and processing of multiple cell types.Methods In this study, we have used 3D bioprinting technology to fabricate cell-laden urethra in vitro with different polymer types and structural characteristics. We hypothesized that use of PCL and PLCL polymers with a spiral scaffold design could mimic the structure and mechanical properties of natural urethra of rabbits, and cell-laden fibrin hydrogel could give a better microenvironment for cell growth. With using an integrated bioprinting system, tubular scaffold was formed with the biomaterials; meanwhile, urothelial cells (UCs) and smooth muscle cells (SMCs) were delivered evenly into inner and outer layers of the scaffold separately within the cell-laden hydrogel.Results The PCL/PLCL (50:50) spiral scaffold demonstrated mechanical properties equivalent to the native urethra in rabbit. Evaluation of the cell bioactivity in the bioprinted urethra revealed that UCs and SMCs maintained more than 80% viability even at 7days after printing. Both cell types also showed active proliferation and maintained the specific biomarkers in the cell-laden hydrogel.Conclusion These results provided a foundation for further studies in 3D bioprinting of urethral constructs that mimic the natural urethral tissue in mechanical properties and cell bioactivity, as well a possibility of using the bioprinted construct for in vivo study of urethral implantation in animal model.Significance of statements The 3D bioprinting is a new technique to replace traditional tissue engineering. The present study is the first demonstration that it is feasible to create a urethral construct. Two kinds of biomaterials were used and achieved mechanical properties equivalent to that of native rabbit urethra. Bladder epithelial cells and smooth muscle cells were loaded in hydrogel and maintained sufficient viability and proliferation in the hydrogel. The highly porous scaffold could mimic a natural urethral base-membrane, and facilitate contacts between the printed epithelial cells and smooth muscle cells on both sides of the scaffold. These results provided a strong foundation for future studies on 3D bioprinted urethra.
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Spiral-structured urethral scaffold made from PCL/PLCL (50:50) has mechanical properties closest to native urethra; cells on scaffold surface maintain good biological activity.
“it was found that the spiral-structured urethral scaffold made from PCL/PLCL (50:50) had mechanical properties closest to those of the native urethra. The cell experiment results showed that the cells on the surface of the scaffold could maintain good biological activity”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [211]
Cites
Abràmoff, M. D., Magalhães, P. J., & Ram, S. J. (2004) · Utrecht University Repository (Utrecht University) · Cited by 11,816 · see citing works
3D bioprinting of tissues and organs
Murphy, S. V., & Atala, A. (2014) · Nature Biotechnology · doi:10.1038/nbt.2958 · Cited by 7,034 · see citing works
Self-citationA 3D bioprinting system to produce human-scale tissue constructs with structural integrity
Kang, H.-W., Lee, S. J., Ko, I. K., Kengla, C., Yoo, J. J., & Atala, A. (2016) · Nature Biotechnology · doi:10.1038/nbt.3413 · Cited by 2,622 · see citing works
Self-citationBiomedical applications of biodegradable polymers
Ulery, B. D., Nair, L. S., & Laurencin, C. T. (2011) · Journal of Polymer Science Part B: Polymer Physics · doi:10.1002/polb.22259 · Cited by 2,206 · see citing works
3D bioprinting for engineering complex tissues
Mandrycky, C., Wang, Z., Kim, K., & Kim, D. (2015) · Biotechnology Advances · doi:10.1016/j.biotechadv.2015.12.011 · Cited by 1,768 · see citing works
Regenerative medicine: Current therapies and future directions
Mao, A. S., & Mooney, D. J. (2015) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1508520112 · Cited by 960 · see citing works
3D Bioprinting for Tissue and Organ Fabrication
Zhang, Y. S., Yue, K., Aleman, J., Mollazadeh‐Moghaddam, K., Bakht, S. M., Yang, J., Jia, W., Dell’Erba, V., Assawes, P., Shin, S. R., et al. (2016) · Annals of Biomedical Engineering · doi:10.1007/s10439-016-1612-8 · Cited by 695 · see citing works
Bioprinted Amniotic Fluid-Derived Stem Cells Accelerate Healing of Large Skin Wounds
Skardal, A., Mack, D. L., Kapetanovic, E., Atala, A., Jackson, J. D., Yoo, J. J., & Söker, S. (2012) · Stem Cells Translational Medicine · doi:10.5966/sctm.2012-0088 · Cited by 663 · see citing works
Self-citationXu, T., Binder, K. W., Albanna, M. Z., Dice, D. D., Zhao, W., Yoo, J. J., & Atala, A. (2012) · Biofabrication · doi:10.1088/1758-5082/5/1/015001 · Cited by 633 · see citing works
Self-citationTissue-engineered autologous urethras for patients who need reconstruction: an observational study
Raya-Rivera, A. M., Esquiliano, D. R., Yoo, J. J., López‐Bayghen, E., Söker, S., & Atala, A. (2011) · The Lancet · doi:10.1016/s0140-6736(10)62354-9 · Cited by 475 · see citing works
Self-citationBioprinting of hybrid tissue constructs with tailorable mechanical properties
Schuurman, W., Khristov, V., Pot, M. W., van Weeren, P. R., Dhert, W. J. A., & Malda, J. (2011) · Biofabrication · doi:10.1088/1758-5082/3/2/021001 · Cited by 411 · see citing works
Bioprinting technology and its applications
Seol, Y., Kang, H.-W., Lee, S. J., Atala, A., & Yoo, J. J. (2014) · European Journal of Cardio-Thoracic Surgery · doi:10.1093/ejcts/ezu148 · Cited by 359 · see citing works
Self-citationAdvances in tissue engineering
Langer, R., & Vacanti, J. P. (2015) · Journal of Pediatric Surgery · doi:10.1016/j.jpedsurg.2015.10.022 · Cited by 317 · see citing works
Biomatrices and biomaterials for future developments of bioprinting and biofabrication
Nakamura, M., Iwanaga, S., Henmi, C., Arai, K., & Nishiyama, Y. (2010) · Biofabrication · doi:10.1088/1758-5082/2/1/014110 · Cited by 311 · see citing works
Rabbit as an animal model for experimental research
Thomas, B., Bhat, K., & Mapara, M. (2012) · Dental Research Journal · doi:10.4103/1735-3327.92960 · Cited by 311 · see citing works
3D-Printed Mechanochromic Materials
Peterson, G. I., Larsen, M. B., Ganter, M. A., Storti, D., & Boydston, A. J. (2014) · ACS Applied Materials & Interfaces · doi:10.1021/am506745m · Cited by 275 · see citing works
Yu, Y., Zhang, Y., Martin, J. A., & Ozbolat, I. T. (2013) · Journal of Biomechanical Engineering · doi:10.1115/1.4024575 · Cited by 268 · see citing works
Male urethral strictures and their management
Hampson, L. A., McAninch, J. W., & Breyer, B. N. (2013) · Nature Reviews Urology · doi:10.1038/nrurol.2013.275 · Cited by 227 · see citing works
Cell-Seeded Tubularized Scaffolds for Reconstruction of Long Urethral Defects: A Preclinical Study
Orabi, H., Aboushwareb, T. A., Zhang, Y., Yoo, J. J., & Atala, A. (2012) · European Urology · doi:10.1016/j.eururo.2012.07.041 · Cited by 131 · see citing works
Self-citationTritschler, S., Roosen, A., Füllhase, C., Stief, C. G., & Rubben, H. (2013) · Deutsches Ärzteblatt international · doi:10.3238/arztebl.2013.0220 · Cited by 121 · see citing works
Tissue Engineering for Human Urethral Reconstruction: Systematic Review of Recent Literature
de Kemp, V. F., de Graaf, P., Fledderus, J. O., Bosch, J. L. H. R., & de Kort, L. M. O. (2015) · PLoS ONE · doi:10.1371/journal.pone.0118653 · Cited by 90 · see citing works
Fu, Q., Deng, C.-L., Zhao, R.-Y., Wang, Y., & Cao, Y. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.09.067 · Cited by 50 · see citing works
Self-citationUreteral Tissue Engineering: Where Are We and How to Proceed?
Simaioforidis, V., de Jonge, P. K. J. D., Sloff, M., Oosterwijk, E., Geutjes, P., & Feitz, W. (2013) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2012.0737 · Cited by 30 · see citing works
Lv, X., Feng, C. M., Fu, Q., Xie, H., Wang, Y., Huang, J., Xie, M., Atala, A., Xu, Y., & Zhao, W. (2015) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.33460 · Cited by 16 · see citing works
Self-citationTissue engineering in urethral reconstruction.
Cui, T., Terlecki, R. P., & Atala, A. (2016) · PubMed · Cited by 11 · see citing works
Self-citationClinical challenges in tissue-engineered urethral reconstruction
Ramsay, S., Goulet, C. R., Langlois, A., & Bolduc, S. (2016) · Translational Andrology and Urology · doi:10.21037/tau.2016.01.11 · Cited by 10 · see citing works
Tissue Engineering of the Urethra
Atala, A. (2008) · Humana Press eBooks · doi:10.1007/978-1-59745-103-1_30 · Cited by 3 · see citing works
Self-citation
Flagged first, then by citations
1 of the 28 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Recent trends in bioinks for 3D printing
Janarthanan, G., & Noh, I. (2018) · Biomaterials Research · doi:10.1186/s40824-018-0122-1 · Cited by 852 · see citing works
3D bioprinting of tissues and organs for regenerative medicine
Vijayavenkataraman, S., Yan, W.-C., Lu, W. F., Wang, C., & Fuh, J. Y. H. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.07.004 · Cited by 634 · see citing works
Hydrogel Bioink Reinforcement for Additive Manufacturing: A Focused Review of Emerging Strategies
Chimene, D., Kaunas, R., & Gaharwar, A. K. (2020) · Advanced Materials · doi:10.1002/adma.201902026 · Cited by 600 · see citing works
Gao, T., Gillispie, G. J., Copus, J. S., Anil Kumar, P. R., Seol, Y., Atala, A., Yoo, J. J., & Lee, S. J. (2018) · Biofabrication · doi:10.1088/1758-5090/aacdc7 · Cited by 519 · see citing works
Self-citationFrom Shape to Function: The Next Step in Bioprinting
Levato, R., Jüngst, T., Scheuring, R. G., Blunk, T., Gröll, J., & Malda, J. (2020) · Advanced Materials · doi:10.1002/adma.201906423 · Cited by 480 · see citing works
PCL-Based Composite Scaffold Matrices for Tissue Engineering Applications
Siddiqui, N., Asawa, S., Birru, B., Baadhe, R. R., & Rao, S. (2018) · Molecular Biotechnology · doi:10.1007/s12033-018-0084-5 · Cited by 461 · see citing works
3D and 4D Printing of Polymers for Tissue Engineering Applications
Tamay, D. G., Usal, T. D., Alagoz, A. S., Yücel, D., Hasırcı, N., & Hasırcı, V. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00164 · Cited by 419 · see citing works
Bio-inks for 3D bioprinting: recent advances and future prospects
Donderwinkel, I., van Hest, J. C. M., & Cameron, N. R. (2017) · Polymer Chemistry · doi:10.1039/c7py00826k · Cited by 383 · see citing works
Bioprinting: From Tissue and Organ Development to in Vitro Models
Mota, C., Camarero‐Espinosa, S., Baker, M., Wieringa, P., & Moroni, L. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.9b00789 · Cited by 308 · see citing works
3D Bioprinted Human Skeletal Muscle Constructs for Muscle Function Restoration
Kim, J. H., Seol, Y., Ko, I. K., Kang, H.-W., Lee, Y. K., Yoo, J. J., Atala, A., & Lee, S. J. (2018) · Scientific Reports · doi:10.1038/s41598-018-29968-5 · Cited by 294 · see citing works
Self-citationRecent Advancements on Three-Dimensional Electrospun Nanofiber Scaffolds for Tissue Engineering
Chen, Y., Dong, X., Shafiq, M., Myles, G., Radacsi, N., & Mo, X. (2022) · Advanced Fiber Materials · doi:10.1007/s42765-022-00170-7 · Cited by 251 · see citing works
Self-citationAdditive Manufacturing of Precision Biomaterials
Guzzi, E. A., & Tibbitt, M. W. (2019) · Advanced Materials · doi:10.1002/adma.201901994 · Cited by 238 · see citing works
Montazerian, H., Mohamed, M. G. A., Montazeri, M. M., Kheiri, S., Milani, A. S., Kim, K., & Hoorfar, M. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.06.040 · Cited by 232 · see citing works
Advanced fabrication for electrospun three-dimensional nanofiber aerogels and scaffolds
Chen, Y., Shafiq, M., Liu, M., Morsi, Y., & Mo, X. (2020) · Bioactive Materials · doi:10.1016/j.bioactmat.2020.06.023 · Cited by 227 · see citing works
Self-citationThree-Dimensional Printing Techniques
Demirak, O., Gökyer, Ş., Ergene, E., & Yilgor, P. (2025) · Biological and medical physics, biomedical engineering · doi:10.1007/978-3-031-90966-5_3 · Cited by 225 · see citing works
Chemical insights into bioinks for 3D printing
Valot, L., Martínez, J., Mehdi, A., & Subra, G. (2019) · Chemical Society Reviews · doi:10.1039/c7cs00718c · Cited by 223 · see citing works
Zhai, X., Ruan, C., Ma, Y., Cheng, D., Wu, M., Liu, W., Zhao, X., Pan, H., & Lu, W. W. (2017) · Advanced Science · doi:10.1002/advs.201700550 · Cited by 199 · see citing works
Bioprinting 101: Design, Fabrication, and Evaluation of Cell-Laden 3D Bioprinted Scaffolds
Deo, K. A., Singh, K. A., Peak, C. W., Alge, D. L., & Gaharwar, A. K. (2020) · Tissue Engineering Part A · doi:10.1089/ten.tea.2019.0298 · Cited by 199 · see citing works
Sánchez, E. M., Gómez-Blanco, J. C., Nieto, E. L., Casado, J. G., Macı́as-Garcı́a, A., Díez, M. A. D., Carrasco-Amador, J. P., Torrejón-Martín, D., Sanchez-Margallo, F. M., & Pagador, J. B. (2020) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2020.00776 · Cited by 187 · see citing works
Mirshafiei, M., Rashedi, H., Yazdian, F. A., Rahdar, A., & Baino, F. (2024) · Materials & Design · doi:10.1016/j.matdes.2024.112853 · Cited by 182 · see citing works
Design, printing, and engineering of regenerative biomaterials for personalized bone healthcare
Jia, Z., Xu, X., Zhu, D., & Zheng, Y. (2023) · Progress in Materials Science · doi:10.1016/j.pmatsci.2023.101072 · Cited by 176 · see citing works
3D printing of tissue engineering scaffolds: a focus on vascular regeneration
Wang, P., Sun, Y., Shi, X., Shen, H., Ning, H., & Liu, H. (2021) · Bio-Design and Manufacturing · doi:10.1007/s42242-020-00109-0 · Cited by 176 · see citing works
3D bioprinting: current status and trends—a guide to the literature and industrial practice
Santoni, S., Gugliandolo, S. G., Sponchioni, M., Moscatelli, D., & Colosimo, B. M. (2021) · Bio-Design and Manufacturing · doi:10.1007/s42242-021-00165-0 · Cited by 172 · see citing works
Clinical Applications of 3D Printing
Ballard, D. H., Trace, A. P., Ali, S., Hodgdon, T., Zygmont, M. E., DeBenedectis, C. M., Smith, S. E., Richardson, M. L., Patel, M. J., Decker, S., et al. (2017) · Academic Radiology · doi:10.1016/j.acra.2017.08.004 · Cited by 174 · see citing works
Functionalizing bioinks for 3D bioprinting applications
Parak, A., Pradeep, P., du Toit, L. C., Kumar, P., Choonara, Y. E., & Pillay, V. (2018) · Drug Discovery Today · doi:10.1016/j.drudis.2018.09.012 · Cited by 170 · see citing works
Recent advances and challenges in materials for 3D bioprinting
Mao, H., Yang, L., Zhu, H., Wu, L., Ji, P., Yang, J., & Gu, Z. (2020) · Progress in Natural Science Materials International · doi:10.1016/j.pnsc.2020.09.015 · Cited by 159 · see citing works
3D printing biomimetic materials and structures for biomedical applications
Zhu, Y., Joralmon, D., Shan, W., Chen, Y., Rong, J., Zhao, H., Xiao, S., & Li, X. (2021) · Bio-Design and Manufacturing · doi:10.1007/s42242-020-00117-0 · Cited by 159 · see citing works
Fang, W., Yang, M., Wang, L., Li, W., Liu, M., Jin, Y., Wang, Y., Yang, R., Wang, Y., Zhang, K., et al. (2023) · International Journal of Bioprinting · doi:10.18063/ijb.759 · Cited by 156 · see citing works
Self-citationNing, L., Sun, H., Lelong, T., Guilloteau, R., Zhu, N., Schreyer, D. J., & Chen, D. (2018) · Biofabrication · doi:10.1088/1758-5090/aacd30 · Cited by 154 · see citing works
Backes, E. H., Harb, S. V., Beatrice, C. A. G., Shimomura, K. M. B., Passador, F. R., Costa, L., & Pessan, L. A. (2021) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.34997 · Cited by 145 · see citing works
Recent advances in 3D printing with protein-based inks
Mu, X., Agostinacchio, F., Xiang, N., Pei, Y., Khan, Y., Guo, C., Cebe, P., Motta, A., & Kaplan, D. L. (2021) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2021.101375 · Cited by 140 · see citing works
Technological advances in fibrin for tissue engineering
Sanz-Horta, R., Matesanz, A., Gallardo, A., Reinecke, H., Jorcano, J. L., Acedo, P., Velasco, D., & Elvira, C. (2023) · Journal of Tissue Engineering · doi:10.1177/20417314231190288 · Cited by 143 · see citing works
Resolution and shape in bioprinting: Strategizing towards complex tissue and organ printing
Lee, J. M., Ng, W. L., & Yeong, W. Y. (2019) · Applied Physics Reviews · doi:10.1063/1.5053909 · Cited by 138 · see citing works
3D Bioprinting Strategies for the Regeneration of Functional Tubular Tissues and Organs
Jeong, H., Nam, H., Jang, J., & Lee, S. (2020) · Bioengineering · doi:10.3390/bioengineering7020032 · Cited by 141 · see citing works
Plant-Derived Biomaterials: A Review of 3D Bioprinting and Biomedical Applications
Jovic, T. H., Kungwengwe, G., Mills, A. C., & Whitaker, I. S. (2019) · Frontiers in Mechanical Engineering · doi:10.3389/fmech.2019.00019 · Cited by 133 · see citing works
Xu, L., Varkey, M., Jorgensen, A. M., Ju, J., Jin, Q., Park, J. H., Fu, Y., Zhang, G., Ke, D., Zhao, W., et al. (2020) · Biofabrication · doi:10.1088/1758-5090/aba2b6 · Cited by 133 · see citing works
Self-citationLeveling Up Hydrogels: Hybrid Systems in Tissue Engineering
Neves, S. C., Moroni, L., Barrias, C. C., & Granja, P. L. (2019) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.09.004 · Cited by 119 · see citing works
Highly Elastic Biodegradable Single-Network Hydrogel for Cell Printing
Xu, C., Lee, W., Dai, G., & Hong, Y. (2018) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.8b01294 · Cited by 119 · see citing works
Wang, Y., Zhang, Y., Li, T., Shen, K., Wang, K. J., Tian, C., & Hu, D. (2023) · Advanced Materials · doi:10.1002/adma.202303642 · Cited by 116 · see citing works
3D Printing Polymers with Supramolecular Functionality for Biological Applications
Pekkanen, A. M., Mondschein, R. J., Williams, C. B., & Long, T. E. (2017) · Biomacromolecules · doi:10.1021/acs.biomac.7b00671 · Cited by 110 · see citing works
Advanced Material Strategies for Next-Generation Additive Manufacturing
Chang, J., He, J., Mao, M., Zhou, W., Lei, Q., Li, X., Li, D., Chua, C.-K., & Zhao, X. (2018) · Materials · doi:10.3390/ma11010166 · Cited by 107 · see citing works
Recent Developments in Biopolymer-Based Hydrogels for Tissue Engineering Applications
Hama, R., Ulziibayar, A., Reinhardt, J. W., Watanabe, T., Kelly, J. M., & Shinoka, T. (2023) · Biomolecules · doi:10.3390/biom13020280 · Cited by 104 · see citing works
Yang, M., Zhang, Y., Fang, C., Song, L., Wang, Y., Lu, L., Yang, R., Bu, Z., Liang, X., Zhang, K., et al. (2022) · Advanced Materials · doi:10.1002/adma.202109522 · Cited by 98 · see citing works
Self-citation3D Bioprinting for Vascularized Tissue-Engineered Bone Fabrication
Xing, F., Xiang, Z., Rommens, P. M., & Ritz, U. (2020) · Materials · doi:10.3390/ma13102278 · Cited by 97 · see citing works
Lee, S. J., Jeong, W., & Atala, A. (2024) · Advanced Materials · doi:10.1002/adma.202408032 · Cited by 102 · see citing works
Self-citationSilva, K. D., Kumar, P., Choonara, Y. E., du Toit, L. C., & Pillay, V. (2020) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36981 · Cited by 94 · see citing works
3D Bioprinted Scaffolds for Tissue Repair and Regeneration
Liu, N., Zhang, X., Guo, Q., Wu, T. S., & Wang, Y. (2022) · Frontiers in Materials · doi:10.3389/fmats.2022.925321 · Cited by 93 · see citing works
Carpio, M. B., Dabaghi, M., Ungureanu, J., Kolb, M. R. J., Hirota, J. A., & Moran-Mirabal, J. M. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.773511 · Cited by 91 · see citing works
3D bioprinting for lungs and hollow organs
Galliger, Z., Vogt, C. D., & Panoskaltsis‐Mortari, A. (2019) · Translational research · doi:10.1016/j.trsl.2019.05.001 · Cited by 85 · see citing works
Cacciamani, G. E., Okhunov, Z., Meneses, A. D., Socarrás, M. E. R., Rivas, J. G., Porpiglia, F., LIATSIKOS, E. N., & Veneziano, D. (2019) · European Urology · doi:10.1016/j.eururo.2019.04.044 · Cited by 85 · see citing works
An Overview of Hydrogel-Based Bioinks for 3D Bioprinting of Soft Tissues
Das, S., & Basu, B. (2019) · Journal of the Indian Institute of Science · doi:10.1007/s41745-019-00129-5 · Cited by 83 · see citing works
Wang, Z., Song, X., Cui, Y., Cheng, K., Tian, X., Dong, M., & Liu, L. (2021) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2021.02.099 · Cited by 78 · see citing works
Cho, H., Jammalamadaka, U. M., & Tappa, K. K. (2018) · Materials · doi:10.3390/ma11020302 · Cited by 71 · see citing works
Liu, G., Fu, M., Li, F., Fu, W., Zhao, Z., Xia, H., & Niu, Y. (2020) · Materials Science and Engineering C · doi:10.1016/j.msec.2020.110810 · Cited by 70 · see citing works
3D Bioprinting of Human Hollow Organs
Panja, N., Maji, S., Choudhuri, S., Ali, K. A., & Hossain, C. M. (2022) · AAPS PharmSciTech · doi:10.1208/s12249-022-02279-9 · Cited by 69 · see citing works
Kurowiak, J., Kaczmarek-Pawelska, A., Mackiewicz, A. G., & Będziński, R. (2020) · Processes · doi:10.3390/pr8030304 · Cited by 68 · see citing works
Zhao, J., Bai, L., Ren, X., Guo, J., Xia, S., Zhang, W., & Feng, Y. (2019) · Acta Biomaterialia · doi:10.1016/j.actbio.2019.07.057 · Cited by 64 · see citing works
Fibrin Matrices as (Injectable) Biomaterials: Formation, Clinical Use, and Molecular Engineering
Roberts, I. V., Bukhary, D. M., Leon‐Valdivieso, C. Y., & Tirelli, N. (2019) · Macromolecular Bioscience · doi:10.1002/mabi.201900283 · Cited by 62 · see citing works
Hedegaard, C. L., & Mata, Á. (2020) · Biofabrication · doi:10.1088/1758-5090/ab84cb · Cited by 60 · see citing works
The potential of 3D printing in urological research and patient care
Colaco, M. A., Igel, D. A., & Atala, A. (2018) · Nature Reviews Urology · doi:10.1038/nrurol.2018.6 · Cited by 59 · see citing works
Self-citationLatest Advances in 3D Bioprinting of Cardiac Tissues
Jafari, A., Ajji, Z., Mousavi, A., Naghieh, S., Bencherif, S. A., & Savoji, H. (2022) · Advanced Materials Technologies · doi:10.1002/admt.202101636 · Cited by 59 · see citing works
Advancements and Challenges in Hydrogel Engineering for Regenerative Medicine
Omidian, H., Chowdhury, S. D., & Wilson, R. L. (2024) · Gels · doi:10.3390/gels10040238 · Cited by 57 · see citing works
New Perspectives of Hydrogels in Chronic Wound Management
Alberts, A., Bratu, A. G., Niculescu, A.-G., & Grumezescu, A. M. (2025) · Molecules · doi:10.3390/molecules30030686 · Cited by 56 · see citing works
Three-dimensional bioprinting of stem-cell derived tissues for human regenerative medicine
Skeldon, G., Lucendo‐Villarin, B., & Shu, W. (2018) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2017.0224 · Cited by 56 · see citing works
Current applications of three‐dimensional printing in urology
Chen, M. Y., Skewes, J., Desselle, M. R., Wong, C. S., Woodruff, M. A., Prokar Dasgupta, A., & Rukin, N. J. (2019) · British Journal of Urology · doi:10.1111/bju.14928 · Cited by 56 · see citing works
An insight on advances and applications of 3d bioprinting: A review
Bejoy, A. M., Makkithaya, K. N., Hunakunti, B. B., Hegde, A., Krishnamurthy, K., Sarkar, A., Lobo, C. F., Keshav, D. V. S., Dharshini, G., Dhivya Dharshini, S., et al. (2021) · Bioprinting · doi:10.1016/j.bprint.2021.e00176 · Cited by 54 · see citing works
Regenerative and engineered options for urethroplasty
Pederzoli, F., Joice, G. A., Salonia, A., Bivalacqua, T. J., & Sopko, N. A. (2019) · Nature Reviews Urology · doi:10.1038/s41585-019-0198-y · Cited by 54 · see citing works
Synthesized biocompatible and conductive ink for 3D printing of flexible electronics
Farizhandi, A. A. K., Khalajabadi, S. Z., Krishnadoss, V., & Noshadi, I. (2020) · Journal of the mechanical behavior of biomedical materials/Journal of mechanical behavior of biomedical materials · doi:10.1016/j.jmbbm.2020.103960 · Cited by 52 · see citing works
Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress
Pedroza-González, S. C., Rodríguez-Salvador, M., Pérez-Benítez, B. E., Álvarez, M. M., & Santiago, G. T. (2021) · International Journal of Bioprinting · doi:10.18063/ijb.v7i2.337 · Cited by 51 · see citing works
Biomimetic models of the glomerulus
Valverde, M. G., Mille, L. S., Figler, K. P., Cervantes, E., Li, V. Y., Bonventre, J. V., Masereeuw, R., & Zhang, Y. S. (2022) · Nature Reviews Nephrology · doi:10.1038/s41581-021-00528-x · Cited by 52 · see citing works
Electrohydrodynamic jet 3D printing of PCL/PVP composite scaffold for cell culture
Li, K., Wang, D., Zhao, K., Song, K., & Liang, J. (2020) · Talanta · doi:10.1016/j.talanta.2020.120750 · Cited by 50 · see citing works
The application of 3D bioprinting in urological diseases
Xu, K., Han, Y., Huang, Y., Wei, P., Yin, J., & Jiang, J. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100388 · Cited by 53 · see citing works
Fibrin-based Bioinks: New Tricks from an Old Dog
Shpichka, A. I., Osipova, D., Efremov, Y. M., Bikmulina, P., Kosheleva, N. V., Lipina, M., Bezrukov, E. A., Sukhanov, R. B., Solovieva, A. B., Vosough, M., et al. (2020) · International Journal of Bioprinting · doi:10.18063/ijb.v6i3.269 · Cited by 49 · see citing works
Wang, Y., Liu, Y., Qian, Y., Lv, L., Li, X., & Liu, Y. (2021) · Surfaces and Interfaces · doi:10.1016/j.surfin.2021.101661 · Cited by 48 · see citing works
Application of poly-ε-caprolactone in extrusion-based bioprinting
Borkar, T., Goenka, V., & Jaiswal, A. K. (2020) · Bioprinting · doi:10.1016/j.bprint.2020.e00111 · Cited by 47 · see citing works
Casarin, M., Morlacco, A., & Moro, F. D. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23126360 · Cited by 47 · see citing works
Romeo, A. A., Easley, J. T., Regan, D. P., Hackett, E. S., Johnson, J. W., Johnson, J. K., Puttlitz, C. M., & McGilvray, K. C. (2021) · Journal of Shoulder and Elbow Surgery · doi:10.1016/j.jse.2021.07.018 · Cited by 47 · see citing works
Recent Advances in Decellularized Matrix-Derived Materials for Bioink and 3D Bioprinting
Liu, H., Gong, Y., Zhang, K., Ke, S., Wang, Y., Wang, J., & Wang, H. (2023) · Gels · doi:10.3390/gels9030195 · Cited by 49 · see citing works
Self-citationBalogová, A. F., Hudák, R., Tóth, T., Schnitzer, M., Feranc, J., Bakoš, D., & Živčák, J. (2018) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2018.08.019 · Cited by 46 · see citing works
3D printing of implantable elastic PLCL copolymer scaffolds
Liu, W., Feng, Z., Ou-Yang, W., Pan, X., Wang, X., Huang, P., Zhang, C., Kong, D., & Wang, W. (2020) · Soft Matter · doi:10.1039/c9sm02396h · Cited by 43 · see citing works
Lee, B., Kim, S., Ko, J., Lee, B., Kim, Y., Park, S., Kim, J., Hyung, S., Kim, H., & Jeon, N. L. (2022) · NPG Asia Materials · doi:10.1038/s41427-022-00355-x · Cited by 43 · see citing works
Three-Dimensional Printing Using a Maize Protein: Zein-Based Inks in Biomedical Applications
Negrete, J. A. T., Aceves-Colin, A. E., Rivera-Flores, D. C., Díaz-Armas, G. G., Mertgen, A.-S., Trinidad-Calderón, P. A., Olmos-Cordero, J. M., Gómez-López, E. G., Pérez‐Carrillo, E., Escobedo-Avellaneda, Z. J., et al. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c00544 · Cited by 43 · see citing works
The current state of tissue engineering in the management of hypospadias
Chan, Y. Y., Bury, M. I., Yura, E. M., Hofer, M. D., Cheng, E. Y., & Sharma, A. K. (2020) · Nature Reviews Urology · doi:10.1038/s41585-020-0281-4 · Cited by 42 · see citing works
Current state of urethral tissue engineering
Mangır, N., Wilson, K. J., Osman, N. I., & Chapple, C. R. (2019) · Current Opinion in Urology · doi:10.1097/mou.0000000000000637 · Cited by 40 · see citing works
The regenerated silk fibroin hydrogel with designed architecture bioprinted by its microhydrogel
Dong, T., Mi, R., Wu, M., Zhong, N., Zhao, X., Chen, X., & Shao, Z. (2019) · Journal of Materials Chemistry B · doi:10.1039/c9tb00783k · Cited by 40 · see citing works
Self-citationThe Importance of Interfaces in Multi‐Material Biofabricated Tissue Structures
Viola, M., Piluso, S., Gröll, J., Vermonden, T., Malda, J., & Castilho, M. D. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202101021 · Cited by 39 · see citing works
Rashidbenam, Z., Jasman, M. H., Hafez, P., Tan, G. H., Goh, E. H., Fam, X. I., Ho, C. C. K., Zainuddin, Z. M., Rajan, R., Nor, F. M., et al. (2019) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-019-00193-z · Cited by 39 · see citing works
Beatrice, C. A. G., Shimomura, K. M. B., Backes, E. H., Harb, S. V., Costa, L., Passador, F. R., & Pessan, L. A. (2020) · Polymer Composites · doi:10.1002/pc.25893 · Cited by 38 · see citing works
Gerdes, S., Ramesh, S., Mostafavi, A., Tamayol, A., Rivero, I. V., & Rao, P. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c00598 · Cited by 38 · see citing works
Wang, L., Cheng, W., Zhu, J., Li, W., Li, D., Yang, X., Zhao, W., Ren, M., Ren, J., Mo, X., et al. (2021) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2021.112218 · Cited by 38 · see citing works
Self-citationSharma, S., & Basu, B. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2021.121331 · Cited by 38 · see citing works
Zhang, K., Bhutto, M. A., Wang, L., Wang, K., Liu, J., Li, W., Cui, W., & Fu, Q. (2023) · Advanced Fiber Materials · doi:10.1007/s42765-022-00252-6 · Cited by 38 · see citing works
Self-citationLi, Z., Chen, Z., Chen, D., & Zhao, R. (2022) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.1c01438 · Cited by 37 · see citing works
Reactive jet impingement bioprinting of high cell density gels for bone microtissue fabrication
Ribeiro, R. D. C., Pal, D., Ferreira, A. M., Gentile, P., Benning, M., & Dalgarno, K. W. (2018) · Biofabrication · doi:10.1088/1758-5090/aaf625 · Cited by 35 · see citing works
Parfenov, V. A., Koudan, E. V., Krokhmal, A. A., Annenkova, E. A., Petrov, S. V., Pereira, F. D. A. S., Karalkin, P. A., Nezhurina, E. K., Gryadunova, A. A., Bulanova, E. A., et al. (2020) · Advanced Healthcare Materials · doi:10.1002/adhm.202000721 · Cited by 34 · see citing works
3D bioprinting in tissue engineering and regenerative medicine
Gupta, S., & Bit, A. (2021) · Cell and Tissue Banking · doi:10.1007/s10561-021-09936-6 · Cited by 34 · see citing works
Biomedical potential of hydrogels: a multifaceted approach to innovative medication delivery
Selvaraj, S., Dutta, V., Chandrasekaran, G., Subbarayan, R., Rana, G., Radhakrishnan, A., Elango, A., & Chauhan, A. (2024) · Emergent Materials · doi:10.1007/s42247-024-00643-y · Cited by 33 · see citing works
Three-Dimensional Printing in Urology: History, Current Applications, and Future Directions
Parikh, N., & Sharma, P. (2018) · Urology · doi:10.1016/j.urology.2018.08.004 · Cited by 31 · see citing works
Hepatocyte culture on 3D porous scaffolds of PCL/PMCL
Xiao, Y., Zhou, M., Zhang, M., Liu, W., Zhou, Y., & Lang, M. (2018) · Colloids and Surfaces B Biointerfaces · doi:10.1016/j.colsurfb.2018.09.064 · Cited by 31 · see citing works
Duan, R., Wang, Y., Zhang, Y., Wang, Z., Du, F., Du, B., Su, D., Liu, L., Li, X., & Zhang, Q. (2021) · ACS Omega · doi:10.1021/acsomega.1c02190 · Cited by 31 · see citing works
- Authors
- Zhang, K., Fu, Q., Yoo, J., Chen, X., Chandra, P., Mo, X., Song, L., Atala, A., & Zhao, W.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Wake Forest University (US)
- Wake Forest University Health Sciences (US)
- Donghua University (CN)
- Nantong University (CN)
- Corresponding author
- Atala, Anthony
- Zhao, Weixin
- ORCID
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2017-03
- Volume
- 50
- Pages
- 154-164
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W2559862105
- Cited by
- 268
- References
- 28
- Citations by year
- 2026: 18 · 2025: 20 · 2024: 25 · 2023: 30 · 2022: 28 · 2021: 44 · 2020: 41 · 2019: 29 · 2018: 25 · 2017: 8
- FWCI
- 9.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Fund of China (81270780, 81470917)
- Science and technology commission of Shanghai (14JC1492100)
- Shanghai Jiaotong University Biomedical Engineering Cross Research Foundation (YG2014ZD07)
- Shanghai Pujiang Plan (14PJD026)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Hydrogels: synthesis, properties, applications
- Keywords
- urethra, urethral stricture, fibrin gel, urothelial cells, smooth muscle cells, urethral tissue engineering, cell viability and proliferation, porous scaffolds, cell-laden hydrogels
- MeSH terms
- Animals, Cell Line, Male, Materials Testing, Polyesters, Rabbits, Urethra, Urothelium, Hydrogels, Tissue Engineering, Myocytes, Smooth Muscle, Biomimetic Materials, Tissue Scaffolds, Printing, Three-Dimensional
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