Beyond the protein corona—Lipids matter for biological response of nanocarriers
Abstract
The interaction of nanocarriers with blood plasma components influences the biological response, and therefore, it needs to be controlled. Whereas protein adsorption to nanocarriers has been investigated to a large extent, the role of lipid interaction for drug delivery and its biological effect is not yet clear. However, lipids represent an important constituent of blood plasma and are usually bound in the form of lipoproteins. Because already for many nanocarrier systems an enrichment of apolipoproteins in their protein corona was reported, we examine the interaction of lipoproteins with nanocarriers. If interaction occurs in terms of lipoprotein adsorption, two scenarios are possible: adsorption of intact lipoprotein complexes or disintegration of the complexes with adsorption of the single components. To investigate the interaction and clarify which scenario occurs, polymeric model nanoparticles and different lipoprotein types have been studied by isothermal titration calorimetry, transmission electron microscopy, and other methods. Our data indicate that upon contact with polymeric nanoparticles, disintegration of lipoproteins and adsorption of lipids occurs. Further, the effect of lipoprotein adsorption on cell uptake has been examined, and a major effect of the lipoproteins has been found.Statement of significance It is now well accepted that nanomaterials developed as diagnostic or therapeutic carrier systems need to be well characterized in terms of biological responses inside an organism. Many studies have already shown that proteins adsorb to the surface of a nanomaterial and create a new interface that define the identity of the material. However, the presence of other surface-active components of the blood plasma and how they interact with nanomaterials has been much less investigated. Thus, this study aims at providing a significant contribution to understanding the interaction mechanism between lipoproteins and nanomaterials. Since lipoproteins transport a high amount of lipids, which are surface-active molecules, the demonstrated interactions can go as far as complete lipoprotein disintegration.
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Serum proteins bind on the surface of synthetic nanoparticle carriers immediately after entering the bloodstream, forming protein coronas.
“It was reported that serum proteins bind on the surface of synthetic nanoparticle (NP) carriers immediately after entering the bloodstream, forming so-called protein coronas [34,35].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [35]
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The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System
Li, T., Li, X., Han, G., Liang, M., Yang, Z., Zhang, C., Huang, S., Tai, S., & Yu, S. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics15010021 · Cited by 110 · see citing works
- Authors
- Müller, J., Prozeller, D., Ghazaryan, A., Kokkinopoulou, M., Mailänder, V., Morsbach, S., & Landfester, K.
- Affiliations
- University Medical Center of the Johannes Gutenberg University Mainz (DE)
- Max Planck Institute for Polymer Research (DE)
- Corresponding author
- Morsbach, Svenja
- Landfester, Katharina
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2018-04
- Volume
- 71
- Pages
- 420-431
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W2792887123
- Cited by
- 79
- References
- 38
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 4 · 2023: 11 · 2022: 8 · 2021: 11 · 2020: 11 · 2019: 15 · 2018: 5
- FWCI
- 3.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- DFG
- Johannes Gutenberg University Mainz
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Lipid Membrane Structure and Behavior
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Interaction Studies and Fluorescence Analysis
- Keywords
- protein corona, lipoproteins, lipid adsorption, nanocarriers, polymeric nanoparticles, cellular uptake, drug delivery, apolipoproteins, nanoparticle biocompatibility
- MeSH terms
- Protein Corona, RAW 264.7 Cells, Animals, Humans, Lipoproteins, Mice, Nanoparticles
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