Early stage mechanical remodeling of collagen surrounding head and neck squamous cell carcinoma spheroids correlates strongly with their invasion capability
Abstract
Mechanical remodeling of stromal collagen, such as reorientation and deformation of collagen matrix, generated by invading cancer cells, plays an important role in the progression of cancer invasion and metastasis. In this study, we applied time-lapse microscopy in conjunction with particle displacement mapping to analyze time-dependent contraction and expansion deformations of collagen surrounding individual spheroids of head and neck squamous cell carcinoma cells (HNSCC), OECM-1 & SAS, as the cancer cells detached from the spheroid and invaded into the surrounding 3D collagen matrix. Our results revealed that highly-invasive HNSCC spheroids, stimulated by epidermal growth factor (EGF), generated a strong contraction deformation of the surrounding collagen in the very early stage, and aligned the collagen fibers radially with respect to the center of the spheroid. This initial collagen contraction deformation generated by the HNSCC spheroid bears a strong positive correlation with the overall extent of subsequent cancer cells invasion; hence, it may serve as an early indicator of the invasion capability of the HNSCC spheroids. STATEMENT OF SIGNIFICANCE: Mechanical remodeling of extracellular matrix (ECM) generated by cancer cells plays an important role in the progression of cancer invasion and metastasis. We observed that the extent of initial contraction deformation of collagen surrounding a head and neck squamous cell carcinoma cell (HNSCC) spheroid played an indispensable role in early stage to promote cancer cells invasion into the surrounding ECM. Our results revealed that more invasive HNSCC spheroids generated a larger extent of initial collagen contraction to align the surrounding collagen and to promote cancer cells invasion. This initial collagen contraction deformation generated by the HNSCC spheroids bears a strong positive correlation with the overall extent of cancer cells invasion; hence, it may serve as an early indicator of the invasion capability of the HNSCC spheroids.
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Tumor spheroids display enhanced deposition of tumor ECM proteins (e.g., fibronectin, lumican, laminin, and collagen type I and VI) compared to 2D cell culture models.
“Several studies have shown that tumor spheroids display an enhanced deposition of tumor ECM proteins (e.g., fibronectin, lumican, laminin, and collagen type I and VI) [118,121,122,123] in comparison to 2D cell culture models.”
From The Tumor-on-Chip: Recent Advances in the Development of Microfluidic Systems to Recapitulate the Physiology of Solid Tumors (2019) · cites this paper as [122]
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- Authors
- Chen, Y.-Q., Kuo, J.-C., Wei, M.-T., Chen, Y.-C., Yang, M.-H., & Chiou, A.
- Affiliations
- National Yang Ming University (TW)
- Princeton University (US)
- Taipei Veterans General Hospital (TW)
- Corresponding author
- Chen, Yin-Quan
- Chiou, Arthur
- Venue
- Acta Biomaterialia
- Publisher
- Elsevier BV
- Published
- 2019-01
- Volume
- 84
- Pages
- 280-292
- ISSN
- 1742-7061 (ISSN-L)
- OpenAlex
- W2902628771
- Cited by
- 29
- References
- 42
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 4 · 2023: 7 · 2022: 5 · 2021: 3 · 2020: 4 · 2019: 1
- FWCI
- 1.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Acta Biomaterialia (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Education
- Ministry of Science and Technology, Taiwan (105-2218-E-010-004, 106-2221-E-010-015-, MOST103-2911-I-009-101)
- National Yang-Ming University
- University of California, San Diego
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Keywords
- head and neck squamous cell carcinoma, tumor invasion, tumor spheroids, extracellular matrix contraction, collagen fiber alignment, epidermal growth factor, SAS cells
- MeSH terms
- Squamous Cell Carcinoma of Head and Neck, Collagen, Head and Neck Neoplasms, Humans, Neoplasm Invasiveness, Neoplasm Proteins, Spheroids, Cellular, Tumor Microenvironment
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