A Simple Hanging Drop Cell Culture Protocol for Generation of 3D Spheroids
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Primary cultures commonly require specific set-ups and complex nutrient media, incorporating ill-defined and variable supplements such as serum.
“They commonly require specific set-ups (Foty, 2011) and complex nutrient media, incorporating ill-defined and variable supplements (such as serum).”
From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Foty, 2011
The Discussion (page unavailable) describes other 3D spheroid methods using simulated microgravity incubators or micro-molded hydrogels, supporting the narrower point that some methods need specific set-ups. It does not establish that primary cultures commonly require them; it says the paper’s own hanging-drop method needs no specialized equipment. The citation is attached to the set-up claim, not the subsequent statement about nutrient media. (LLM, conf=0.82)
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Cites
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The differential adhesion hypothesis: a direct evaluation
Foty, R. A., & Steinberg, M. S. (2005) · Developmental Biology · doi:10.1016/j.ydbio.2004.11.012 · Cited by 908 · see citing works
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Research works printed but not linked: 4
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Nunes, A. S., Barros, A. S., Costa, E. C., Moreira, A. F., & Correia, I. J. (2019) · Biotechnology and Bioengineering · doi:10.1002/bit.26845 · Cited by 721 · see citing works
Spheroid Culture of Mesenchymal Stem Cells
Cesarz, Z., & Tamama, K. (2016) · Stem Cells International · doi:10.1155/2016/9176357 · Cited by 475 · see citing works
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Han, S. J., Kwon, S., & Kim, K. S. (2021) · Cancer Cell International · doi:10.1186/s12935-021-01853-8 · Cited by 428 · see citing works
The production of 3D tumor spheroids for cancer drug discovery
Sant, S., & Johnston, P. A. (2017) · Drug Discovery Today: Technologies · doi:10.1016/j.ddtec.2017.03.002 · Cited by 404 · see citing works
Bioprinting towards physiologically relevant tissue models for pharmaceutics
Peng, W., Unutmaz, D., & Ozbolat, I. T. (2016) · Trends in Biotechnology · doi:10.1016/j.tibtech.2016.05.013 · Cited by 196 · see citing works
3D scaffolds in breast cancer research
Rijal, G., & Li, W. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2015.12.016 · Cited by 169 · see citing works
Vörsmann, H., Groeber, F., Walles, H., Busch, S., Beissert, S., Walczak, H., & Kulms, D. (2013) · Cell Death & Disease · doi:10.1038/cddis.2013.249 · Cited by 168 · see citing works
“Melanoma spheroids were generated via the ‘hanging drop’ method incorporating 250 melanoma cells per 25ml RPMI placed on the lid of a non-adhesive PBS containing petri dish.(15) Spheroids were incubated for 15 days at 371C, 5% CO2.”
MethodA Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting
Berens, E. B., Holy, J. M., Riegel, A. T., & Wellstein, A. (2015) · Journal of Visualized Experiments · doi:10.3791/53409 · Cited by 112 · see citing works
Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0)
Pamies, D. (2021) · ALTEX · doi:10.14573/altex.2111011 · Cited by 100 · see citing works
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In vitro modeling of the prostate cancer microenvironment
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3D Tumor Spheroid Models for In Vitro Therapeutic Screening of Nanoparticles
Daunys, S., Janonienė, A., Januškevičienė, I., Paškevičiūtė, M., & Petrikaitė, V. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-58174-9_11 · Cited by 32 · see citing works
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Fabrication of micro-cages and caged tumor spheroids for microfluidic chip-based assays
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Wei, B., Troendle, E. P., Simpson, D. A., & Stitt, A. W. (2026) · PLoS ONE · doi:10.1371/journal.pone.0345045
- Authors
- Foty, R.
- Venue
- Journal of Visualized Experiments
- Publisher
- MyJove Corporation
- Published
- 2011-05-06
- Issue
- 51
- ISSN
- 1940-087X
- DOI
- 10.3791/2720
- Cited by
- 263
- References
- 2
- Language
- en
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