Cited by 172Extracellular vesicles in diseaseMesenchymal stem cell researchPeriodontal Regeneration and Treatments

The tissue origin effect of extracellular vesicles on cartilage and bone regeneration

Li, Qi, Yu, Huilei, Sun, Muyang, Yang, Peng, Hu, Xiaoqing, Ao, Yingfang, Cheng, Jin·2021·Acta Biomaterialia

Abstract

Direct implantation of mesenchymal stem cells (MSCs) for cartilage and bone tissue engineering faces challenges, such as immune rejection and loss of cellular viability or functionality. As nanoscale natural particles, exosomes or small extracellular vesicles (EVs) of MSCs have potential to circumvent these problems. It is significant to investigate the impact of the tissue origin of MSCs on the therapeutic bioactivity of their corresponding EVs for cartilage and bone regeneration. Here, rat MSCs isolated from the adipose, bone marrow, and synovium are cultured to obtain their corresponding EVs (ADSC-EVs, BMSC-EVs, and SMSC-EVs, respectively). The ADSC-EVs stimulate the migration, proliferation, and chondrogenic and osteogenic differentiation of BMSCs in vitro as well as cartilage and bone regeneration in a mouse model more than the BMSC-EVs or SMSC-EVs. Proteomics analysis reveals that the tissue origin contributes to the distinct protein profiles among the three types of EVs, which induced cartilage and bone regenerative capacities by potential mechanisms of regulating signaling pathways including focal adhesion, ECM-receptor interaction, actin cytoskeleton, cAMP, and PI3K-Akt signaling pathways. Consequently, these findings provide insight that the adipose may be a superior candidate in EV-based nanomedicine for cartilage and bone regeneration. STATEMENT OF SIGNIFICANCE: Extracelluar vesicles (EVs) of mesenchymal stem cells (MSCs) have been considered as a promising approach in cartilage and bone tissue engineering. In this study, for the first time, we investigated the tissue origin effect of EVs on chondrogenesis and osteogenesis of MSCs in vitro and in vivo. The results demonstrated that EVs of adipose-derived MSCs showed the most efficiency. Meanwhile, protein proteomics revealed the potential mechanisms. We provide a novel evidence that the adipose is a superior reservoir in EV-based nanotechnologies and biomaterials for cartilage and bone regeneration.

Integrity signals

Registry

Crossref · 10.1016/j.actbio.2021.02.039

Cites

68 of 68 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Li, Q., Yu, H., Sun, M., Yang, P., Hu, X., Ao, Y., & Cheng, J.
Affiliations
Peking University (CN)
Corresponding author
  • Ao, Yingfang
  • Cheng, Jin
Venue
Acta Biomaterialia
Publisher
Elsevier BV
Published
2021-04
Volume
125
Pages
253-266
ISSN
1742-7061 (ISSN-L)
OpenAlex
W3134379672
Cited by
172
References
68
Citations by year
2026: 12 · 2025: 30 · 2024: 61 · 2023: 31 · 2022: 27 · 2021: 11
FWCI
10.20
Type
journal article
Language
en
Open access
closed
Locations
  • Acta Biomaterialia (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Beijing Municipal Science and Technology Commission
  • National Natural Science Foundation of China
  • Beijing Natural Science Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
Keywords
extracellular vesicles, mesenchymal stem cells, tissue of origin, cartilage regeneration, bone regeneration, adipose-derived stem cells, bone marrow-derived stem cells, synovial stem cells, chondrogenic differentiation, osteogenic differentiation, EV proteomics, signaling pathways
MeSH terms
Extracellular Vesicles, Animals, Bone Regeneration, Cartilage, Cell Differentiation, Osteogenesis, Phosphatidylinositol 3-Kinases, Chondrogenesis, Mice, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review