Anchor peptide captures, targets, and loads exosomes of diverse origins for diagnostics and therapy
Abstract
further increased dystrophin expression in muscle with functional improvement without any detectable toxicity. Our study demonstrates that an exosomal anchor peptide enables direct, effective functionalization and capture of exosomes, thus providing a tool for exosome engineering, probing gene function in vivo, and targeted therapeutic drug delivery.
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siRNAs located on either side of the EV membrane can induce gene knockdown, indicating both routes are possible.
“the ability of siRNAs located on either side of the EV membrane to induce gene knockdown indicates both routes are possible15,16.”
From GAPDH controls extracellular vesicle biogenesis and enhances the therapeutic potential of EV mediated siRNA delivery to the brain (2021) · cites this paper as [16]
Targeting CP05-loaded exosomes increased delivery of splice-correcting oligomers to muscle, thereby restoring muscular dystrophy.
“Following systemic administration of CP05-loaded exosomes, researchers found that targeting CP05-loaded exosomes increased the delivery of splice-correcting oligomers to muscle, thereby restoring muscular dystrophy [27].”
From The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System (2022) · cites this paper as [27]
Cites
Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes
Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., & Wood, M. J. A. (2011) · Nature Biotechnology · doi:10.1038/nbt.1807 · Cited by 4,930 · see citing works
Self-citationExosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer
Kamerkar, S., LeBleu, V. S., Sugimoto, H., Yang, S., Ruivo, C. F., Melo, S. A., Lee, J. J., & Kalluri, R. (2017) · Nature · doi:10.1038/nature22341 · Cited by 2,639 · see citing works
Witwer, K. W., Buzás, E. I., Bemis, L., Bora, A., Lässer, C., Lötvall, J. O., Hoen, E. N. M. N., Piper, M. G., Sivaraman, S., Skog, J., et al. (2013) · Journal of Extracellular Vesicles · doi:10.3402/jev.v2i0.20360 · Cited by 2,424 · see citing works
Syndecan–syntenin–ALIX regulates the biogenesis of exosomes
Baietti, M. F., Zhang, Z., Mortier, E., Melchior, A., Degeest, G., Geeraerts, A., Ivarsson, Y., Depoortere, F., Coomans, C., Vermeiren, E., et al. (2012) · Nature Cell Biology · doi:10.1038/ncb2502 · Cited by 1,899 · see citing works
Tian, Y., Li, S., Song, J., Ji, T., Zhu, M., Anderson, G. J., Wei, J., & Nie, G. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.11.083 · Cited by 1,809 · see citing works
Abels, E. R., & Breakefield, X. O. (2016) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-016-0366-z · Cited by 1,685 · see citing works
Development of exosome-encapsulated paclitaxel to overcome MDR in cancer cells
Kim, M. S., Haney, M. J., Zhao, Y., Mahajan, V., Le‐Deygen, I. M., Klyachko, N. L., Inskoe, E., Piroyan, A., Sokolsky‐Papkov, M., Okolie, O., et al. (2015) · Nanomedicine Nanotechnology Biology and Medicine · doi:10.1016/j.nano.2015.10.012 · Cited by 1,498 · see citing works
Tetraspanins in Extracellular Vesicle Formation and Function
Andreu, Z., & Yáñez-Mó, M. (2014) · Frontiers in Immunology · doi:10.3389/fimmu.2014.00442 · Cited by 1,401 · see citing works
Using exosomes, naturally-equipped nanocarriers, for drug delivery
Batrakova, E. V., & Kim, M. S. (2015) · Journal of Controlled Release · doi:10.1016/j.jconrel.2015.07.030 · Cited by 1,122 · see citing works
Tumor-Derived Exosomes and Their Role in Cancer Progression
Whiteside, T. L. (2016) · Advances in clinical chemistry · doi:10.1016/bs.acc.2015.12.005 · Cited by 762 · see citing works
Johnsen, K. B., Gudbergsson, J. M., Skov, M. N., Pilgaard, L., Moos, T., & Duroux, M. (2014) · Biochimica et Biophysica Acta (BBA) - Reviews on Cancer · doi:10.1016/j.bbcan.2014.04.005 · Cited by 704 · see citing works
Kooijmans, S. A. A., Stremersch, S., Braeckmans, K., de Smedt, S. C., Hendrix, A., Wood, M. J. A., Schiffelers, R. M., Raemdonck, K., & Vader, P. (2013) · Journal of Controlled Release · doi:10.1016/j.jconrel.2013.08.014 · Cited by 663 · see citing works
van der Vlist, E. J., Hoen, E. N. M. N., Stoorvogel, W., Arkesteijn, G. J. A., & Wauben, M. H. M. (2012) · Nature Protocols · doi:10.1038/nprot.2012.065 · Cited by 534 · see citing works
Surface Functionalization of Exosomes Using Click Chemistry
Smyth, T., Petrova, K., Payton, N. M., Persaud, I., Redzic, J. S., Graner, M. W., Smith-Jones, P., & Anchordoquy, T. J. (2014) · Bioconjugate Chemistry · doi:10.1021/bc500291r · Cited by 532 · see citing works
Extracellular vesicles as drug delivery systems: Lessons from the liposome field
van der Meel, R., Fens, M. H. A. M., Vader, P., van Solinge, W. W., Eniola‐Adefeso, O., & Schiffelers, R. M. (2014) · Journal of Controlled Release · doi:10.1016/j.jconrel.2014.07.049 · Cited by 486 · see citing works
Exosome-mediated delivery of hydrophobically modified siRNA for huntingtin mRNA silencing
Didiot, M.-C., Hall, L. M., Coles, A. H., Haraszti, R. A., Godinho, B. M., Chase, K., Sapp, E., Ly, S., Alterman, J. F., Hassler, M. R., et al. (2016) · Molecular Therapy · doi:10.1038/mt.2016.126 · Cited by 483 · see citing works
Blood Exosomes Endowed with Magnetic and Targeting Properties for Cancer Therapy
Qi, H., Liu, C., Long, L., Ren, Y., Zhang, S., Chang, X., Qian, X., Jia, H., Zhao, J., Sun, J., et al. (2016) · ACS Nano · doi:10.1021/acsnano.5b06939 · Cited by 482 · see citing works
Lu, Z. F., Zuo, B., Jing, R., Gao, X., Rao, Q., Liu, Z., Han, Q., Guo, H., & Yin, H. (2017) · Journal of Hepatology · doi:10.1016/j.jhep.2017.05.019 · Cited by 391 · see citing works
Self-citationDuchenne muscular dystrophy and dystrophin: pathogenesis and opportunities for treatment
Nowak, K. J., & Davies, K. E. (2004) · EMBO Reports · doi:10.1038/sj.embor.7400221 · Cited by 358 · see citing works
Fluorescent peptides highlight peripheral nerves during surgery in mice
Whitney, M., Crisp, J. L., Nguyen, L. T., et al. (2011) · Nature Biotechnology · doi:10.1038/nbt.1764 · Cited by 207 · see citing works
Development of exosome surface display technology in living human cells
Stickney, Z., Losacco, J., McDevitt, S., Zhang, Z., & Lü, B. (2016) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2016.02.058 · Cited by 200 · see citing works
Therapeutic and diagnostic applications of extracellular vesicles
Stremersch, S., Smedt, S. C. D., & Raemdonck, K. (2016) · Journal of Controlled Release · doi:10.1016/j.jconrel.2016.07.054 · Cited by 187 · see citing works
PepBank - a database of peptides based on sequence text mining and public peptide data sources.
Shtatland, T., Guettler, D., Kossodo, M., Pivovarov, M., & Weissleder, R. (2007) · BMC Bioinformatics · doi:10.1186/1471-2105-8-280 · Cited by 181 · see citing works
Hepatocellular carcinoma cell-specific peptide ligand for targeted drug delivery
Lo, A., Lin, C., & Wu, H. (2008) · Molecular Cancer Therapeutics · doi:10.1158/1535-7163.mct-07-2359 · Cited by 180 · see citing works
Ivanova, G. D., Arzumanov, A. A., Abes, R., et al. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn671 · Cited by 176 · see citing works
Zatz, M., Rapaport, D., Vainzof, M., Passos-Bueno, M. R., Bortolini, E. R., Pavanello, R. D. C. M., & Peres, C. A. (1991) · Journal of the Neurological Sciences · doi:10.1016/0022-510x(91)90068-i · Cited by 159 · see citing works
Morpholino Oligomer–Mediated Exon Skipping Averts the Onset of Dystrophic Pathology in the mdx Mouse
Fletcher, S., Honeyman, K., Fall, A., Harding, P. L., Johnsen, R. D., Steinhaus, J. P., Moulton, H. M., Iversen, P. L., & Wilton, S. D. (2007) · Molecular Therapy · doi:10.1038/sj.mt.6300245 · Cited by 158 · see citing works
Self-citationSAROTUP: Scanner and Reporter of Target-Unrelated Peptides
Huang, J. H., Ru, B., Li, S., et al. (2010) · BioMed Research International · doi:10.1155/2010/101932 · Cited by 117 · see citing works
Ferri, P., Barbieri, E., Burattini, S., et al. (2009) · Journal of Cellular Biochemistry · doi:10.1002/jcb.22360 · Cited by 112 · see citing works
What Can We Learn From Clinical Trials of Exon Skipping for DMD?
Lu, Q., Çırak, S., & Partridge, T. A. (2014) · Molecular Therapy — Nucleic Acids · doi:10.1038/mtna.2014.6 · Cited by 92 · see citing works
Gao, X., Zhao, J., Han, G., Zhang, Y., Dong, X., Cao, L., Wang, Q., Moulton, H. M., & Yin, H. (2014) · Molecular Therapy · doi:10.1038/mt.2014.63 · Cited by 71 · see citing works
Self-citationExosomes mediate embryo and maternal interactions at implantation and during pregnancy
Nowak, R. A. (2016) · Frontiers in Bioscience-Scholar · doi:10.2741/s448 · Cited by 58 · see citing works
Isolation of Peptides that Can Recognize Syndiotactic Polystyrene
Serizawa, T., Techawanitchai, P., & Matsuno, H. (2007) · ChemBioChem · doi:10.1002/cbic.200700133 · Cited by 51 · see citing works
Hexose enhances oligonucleotide delivery and exon skipping in dystrophin-deficient mdx mice
Han, G., Gu, B., Cao, L., et al. (2016) · Nature Communications · doi:10.1038/ncomms10981 · Cited by 48 · see citing works
Bounda, G., Wang, Z., Wang, D., et al. (2015) · Evidence-based Complementary and Alternative Medicine · doi:10.1155/2015/329831 · Cited by 29 · see citing works
Flagged first, then by citations
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Sorted by citations
RNA delivery by extracellular vesicles in mammalian cells and its applications
O’Brien, K., Breyne, K., Ughetto, S., Laurent, L. C., & Breakefield, X. O. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0251-y · Cited by 1,811 · see citing works
Zhang, Y., Bi, J., Huang, J., Tang, Y., Du, S., & Li, P. (2020) · International Journal of Nanomedicine · doi:10.2147/ijn.s264498 · Cited by 1,323 · see citing works
Advances in therapeutic applications of extracellular vesicles
Wiklander, O. P. B., Brennan, M. Á., Lötvall, J., Breakefield, X. O., & EL Andaloussi, S. (2019) · Science Translational Medicine · doi:10.1126/scitranslmed.aav8521 · Cited by 1,039 · see citing works
Engineered Extracellular Vesicles for Cancer Therapy
Zhang, X., Zhang, H., Gu, J., Zhang, J., Shi, H., Qian, H., Wang, D., Xu, W., Pan, J., & Santos, H. A. (2021) · Advanced Materials · doi:10.1002/adma.202005709 · Cited by 428 · see citing works
Extracellular vesicles: A bright star of nanomedicine
Wu, P., Zhang, B., Ocansey, D. K. W., Xu, W., & Qian, H. (2021) · Biomaterials · doi:10.1016/j.biomaterials.2020.120467 · Cited by 368 · see citing works
Approaches to surface engineering of extracellular vesicles
Richter, M., Vader, P., & Fuhrmann, G. (2021) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2021.03.020 · Cited by 258 · see citing works
Pham, T. C., Jayasinghe, M. K., Pham, T. T., Yang, Y., Wei, L., Usman, W. M., Chen, H., Pirisinu, M., Gong, J., Kim, S., et al. (2021) · Journal of Extracellular Vesicles · doi:10.1002/jev2.12057 · Cited by 250 · see citing works
Zha, Y., Li, Y., Lin, T., Chen, J., Zhang, S., & Wang, J. (2021) · Theranostics · doi:10.7150/thno.50741 · Cited by 232 · see citing works
Liu, Q., Li, D., Pan, X., & Liang, Y. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-02081-0 · Cited by 201 · see citing works
The tissue origin effect of extracellular vesicles on cartilage and bone regeneration
Li, Q., Yu, H., Sun, M., Yang, P., Hu, X., Ao, Y., & Cheng, J. (2021) · Acta Biomaterialia · doi:10.1016/j.actbio.2021.02.039 · Cited by 172 · see citing works
Liang, Y., Iqbal, Z., Lu, J., Wang, J., Zhang, H., Chen, X., Duan, L., & Xia, J. (2023) · Molecular Therapy · doi:10.1016/j.ymthe.2022.10.008 · Cited by 162 · see citing works
Hydrogel armed with Bmp2 mRNA-enriched exosomes enhances bone regeneration
Yang, Z., Li, X., Gan, X., Wei, M., Wang, C., Yang, G., Zhao, Y., Zhu, Z., & Wang, Z. (2023) · Journal of Nanobiotechnology · doi:10.1186/s12951-023-01871-w · Cited by 109 · see citing works
The Therapeutic Potential and Clinical Significance of Exosomes as Carriers of Drug Delivery System
Li, T., Li, X., Han, G., Liang, M., Yang, Z., Zhang, C., Huang, S., Tai, S., & Yu, S. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics15010021 · Cited by 110 · see citing works
Strategies for the use of Extracellular Vesicles for the Delivery of Therapeutics
Sil, S., Dagur, R. S., Liao, K., Peeples, E. S., Hu, G., Periyasamy, P., & Buch, S. (2020) · Journal of Neuroimmune Pharmacology · doi:10.1007/s11481-019-09873-y · Cited by 109 · see citing works
Dar, G. H., Mendes, C. C., Kuan, W.-L., Speciale, A. A., Conceição, M., Görgens, A., Uliyakina, I., Lobo, M. J., Lim, W. F., EL Andaloussi, S., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-27056-3 · Cited by 100 · see citing works
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Ojeda-Hernández, D. D., Hernández-Sapiéns, M. A., Reza-Zaldívar, E. E., Canales-Aguirre, A., Matías-Guiu, J. A., Matías-Guiu, J., Mateos-Díaz, J. C., Gómez-Pinedo, U., & Sancho-Bielsa, F. (2022) · Life · doi:10.3390/life12091417 · Cited by 25 · see citing works
- Authors
- Gao, X., Ran, N., Dong, X., Zuo, B., Yang, R., Zhou, Q., Moulton, H. M., Seow, Y., & Yin, H.
- Affiliations
- Tianjin Medical University (CN)
- Huazhong University of Science and Technology (CN)
- Oregon State University (US)
- Agency for Science, Technology and Research (SG)
- Corresponding author
- Gao, Xianjun
- Ran, Ning
- Yin, HaiFang
- Venue
- Science Translational Medicine
- Published
- 2018-06-06
- Volume
- 10
- Issue
- 444
- ISSN
- 1946-6234 (ISSN-L)
- 1946-6242
- OpenAlex
- W2806012625
- Cited by
- 426
- References
- 35
- Citations by year
- 2026: 48 · 2025: 58 · 2024: 71 · 2023: 56 · 2022: 78 · 2021: 53 · 2020: 40 · 2019: 20 · 2018: 2
- FWCI
- 10.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science Translational Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (81672124, 81671528, 81361128013, 81301526, 81273420)
- National key R&D program of China (2017YFC1001902)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- exosomes, exosome capture, exosome engineering, targeted drug delivery, exosome-based diagnostics, dystrophin expression
- MeSH terms
- Animals, Cell Line, Inflammation, Mice, Inbred C57BL, Muscles, Peptides, Mice, Inbred mdx, Serum, Exosomes, Tetraspanin 30, Morpholinos
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