Addressing the soil carbon dilemma: Legumes in intensified rotations regenerate soil carbon while maintaining yields in semi-arid dryland wheat farms
Open accessAbstract
Semi-arid regions support the majority of global wheat production, but they are threatened by continued soil erosion and increased risk of drought and extreme temperatures from climate change. Enhancing soil organic matter (SOM) is a key adaptation strategy for sustaining agriculture in these regions because it can improve water retention and nutrient cycling. When seeking to increase SOM, growers face a dilemma, known as the ‘soil carbon (C) dilemma’ (Janzen, 2006): How can SOM be increased, while also increasing the release of nutrients that accompanies decomposition? Our observational study examined whether growers could address this dilemma through intensification with diverse grain crops. We specifically tested whether incorporating legumes into a continuous rotation influences the form and amount of SOM as well as productivity in farms of the central Great Plains region of the U.S. by contrasting three, no-till rotation systems: 1) conventional wheat-fallow; 2) continuous grain-only rotations, and 3) continuous grain rotations that incorporate a legume crop. We sampled on-farm fields and experimental agricultural research station plots that had received one of these rotations for at least eight years. We compared yield, soil organic C (SOC), and nitrogen (N) stocks in bulk soil to 1 m, as well as SOC and N in particulate organic matter (POM) and mineral-associated organic matter (MAOM) in surface soils (0–10 cm) among the different rotations. We also estimated the long-term effects of the rotations on SOC fractions through a simulation of each rotation with the MEMS 1.0 model, verified with measured data. We found that intensifying the rotation with continuous grains led to 1.5-fold increase in aggregate size but did not change SOC stocks. Incorporating a legume to the continuous grain rotation resulted in 1 Mg C ha-1 more SOC on average in surface soil compared to wheat-fallow rotations. No significant soil changes among rotations were observed at depth. Longer-term simulations of these cropping systems suggest that including legumes could allow for 10% greater SOC gains over time compared to wheat-fallow. Since legume incorporation also increased crop yields across the full rotation cycle, our results support our hypothesis that growers can solve the ‘soil C dilemma’ by simultaneously increasing the quantity and N-content of soil input through continuous cropping with both grains and legumes.
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Van der Pol et al. (2022) found that converting a fallow-grain crop rotation to a continuous grain crop rotation increased grain yield and SOC stocks only when a legume crop was also included in the rotation.
“This was affirmed by another recent study (van der Pol et al., 2022), who found that converting a fallow- grain crop rotation to a continuous grain crop rotation increased grain yield and SOC stocks only when a legume crop was also included in the rotation (enhancing inputs from biological N 2 fixation).”
From Carbon for soils, not soils for carbon (2023) · cites this paper as van der Pol et al., 2022
The Abstract reports that continuous grains without legumes did not change SOC stocks, while adding a legume increased surface-soil SOC relative to wheat–fallow. But section 4.2 says annualized crop yields increased under both continuous rotations, so the claim that yield increased only with a legume is not supported. (LLM, conf=0.88)
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Unlocking complex soil systems as carbon sinks: multi-pool management as the key
Angst, G., Mueller, K. E., Castellano, M. J., Vogel, C., Wiesmeier, M., & Mueller, C. W. (2023) · Nature Communications · doi:10.1038/s41467-023-38700-5 · Cited by 474 · see citing works
Carbon for soils, not soils for carbon
Moinet, G. Y. K., Hijbeek, R., van Vuuren, D. P., & Giller, K. E. (2023) · Global Change Biology · doi:10.1111/gcb.16570 · Cited by 249 · see citing works
“This was affirmed by another recent study (van der Pol et al., 2022), who found that converting a fallow- grain crop rotation to a continuous grain crop rotation increased grain yield and SOC stocks only when a legume crop was also included in the rotation (enhancing inputs from biological N2…”
ResultHansen, P. M., Even, R., King, A. E., Lavallee, J., Schipanski, M., & Cotrufo, M. F. (2024) · Global Change Biology · doi:10.1111/gcb.17080 · Cited by 150 · see citing works
Self-citationJust, C., Armbruster, M., Barkusky, D., Baumecker, M., Diepolder, M., Döring, T. F., Heigl, L., Honermeier, B., Jate, M., Merbach, I., et al. (2023) · Geoderma · doi:10.1016/j.geoderma.2023.116472 · Cited by 88 · see citing works
Jilling, A., Grandy, A. S., Daly, A. B., Hestrin, R., Possinger, A. R., Abramoff, R., Annis, M., Cates, A. M., Dynarski, K. A., Georgiou, K., et al. (2025) · Communications Earth & Environment · doi:10.1038/s43247-025-02681-8 · Cited by 68 · see citing works
Climate Change Impacts on Legume Physiology and Ecosystem Dynamics: A Multifaceted Perspective
Dave, K., Kumar, A., Dave, N., Jain, M., Dhanda, P. S., Yadav, A., & Kaushik, P. (2024) · Sustainability · doi:10.3390/su16146026 · Cited by 69 · see citing works
Wang, Y., Shi, M., Zhang, R., Zhang, W., Liu, Y., Sun, D., Wang, X., Qin, S., & Kang, Y. (2023) · Chemical and Biological Technologies in Agriculture · doi:10.1186/s40538-023-00508-2 · Cited by 49 · see citing works
Qian, R., Guo, R., Liu, Y., Naseer, M. A., Hussain, S., Liu, D., Zhang, P., Chen, X.-L., & Ren, X.-L. (2022) · Agriculture, Ecosystems & Environment · doi:10.1016/j.agee.2022.108181 · Cited by 30
Iheshiulo, E. M., Larney, F. J., Hernandez‐Ramirez, G., Luce, M. S., Chau, H. W., & Liu, K. (2024) · Geoderma · doi:10.1016/j.geoderma.2024.116777 · Cited by 33 · see citing works
Importance of on-farm research for validating process-based models of climate-smart agriculture
Ellis, E., & Paustian, K. H. (2024) · Carbon Balance and Management · doi:10.1186/s13021-024-00260-6 · Cited by 33 · see citing works
“…recent studies have worked collaboratively with agricultural producers across select regions to sample commercial fields representing a broader range of climate-smart practices than are currently established in LTEs or to compare LTE results to on-farm realities [5, 17, 18, 34, 54, 66].”
BackgroundMeta-analysis shows that planting nitrogen-fixing species increases soil organic carbon stock
Sun, X., Chen, J., Kuzyakov, Y. V., Yang, Y., Ferreira, G. W. D., Ochoa‐Hueso, R., Mueller, C. W., Wang, Z., Hong, Y., Li, D., et al. (2025) · Nature Ecology & Evolution · doi:10.1038/s41559-025-02861-x · Cited by 21 · see citing works
Shumba, A., Chikowo, R., Thierfelder, C., Corbeels, M., Six, J., & Cardinael, R. (2024) · SOIL · doi:10.5194/soil-10-151-2024 · Cited by 31 · see citing works
Domnariu, H., Reardon, C. L., Manning, V. A., Gollany, H. T., & Trippe, K. M. (2024) · Agriculture Ecosystems & Environment · doi:10.1016/j.agee.2024.108959 · Cited by 26 · see citing works
Wu, Q., Lawley, Y., & Congreves, K. (2024) · Agriculture, Ecosystems & Environment · doi:10.1016/j.agee.2023.108755 · Cited by 17
Ishtiaque, A. (2023) · Environmental Research Climate · doi:10.1088/2752-5295/accb03 · Cited by 17 · see citing works
Liu, W., Shi, Z., He, H.-Y., Lin, B., He, C., Dang, Y. P., Zhao, X., & Zhang, H. (2025) · Soil and Tillage Research · doi:10.1016/j.still.2025.106792 · Cited by 13 · see citing works
Panagea, I., Quataert, P., Alonso‐Ayuso, M., Bárcena, T. G., Boever, M. D., Diacono, M., Jacobs, A., Jensen, J. L., Seidel, F., Seitz, D., et al. (2025) · European Journal of Soil Science · doi:10.1111/ejss.70086 · Cited by 17 · see citing works
Murindangabo, Y., Kopecký, M., Perná, K., Nguyen, T. G., Ghorbani, M., Konvalina, P., Bohatá, A., Kavková, M., Hoang, T. N., Kabelka, D., et al. (2023) · Agriculture · doi:10.3390/agriculture13071314 · Cited by 12
Liu, C., Zhang, L., Zhou, J., Xu, Y., Yang, Y., Zeng, Z.-H., & Zang, H.-D. (2024) · ACTA AGRONOMICA SINICA · doi:10.3724/sp.j.1006.2024.34195 · Cited by 8
Do carbon farming practices build bioavailable nitrogen pools?
Mattila, T., Girz, A. I., & Pihlatie, M. (2023) · Soil use and management · doi:10.1111/sum.12930 · Cited by 8
“In contrast, carbon farming practises that include legumes (van der Pol et al., 2022) or recycle otherwise leachable N (Aronsson et al., 2016) seem to build labile N pools and increase N mineralisation to crop plants.”
Seasonal soil health dynamics in soy-wheat relay intercropping
Thompson, J. B., Döring, T. F., Bowles, T. M., Kolb, S., Bellingrath‐Kimura, S. D., & Reckling, M. (2024) · Scientific Reports · doi:10.1038/s41598-024-69903-5 · Cited by 8 · see citing works
“The addition of legumes to a continuous grain cropping system in the American Midwest was found to increase MAOM C which was partially attributed to legume exudates and lower C:N ratio of legume residue 34 .”
Fernández-Ortega, J., Álvaro‐Fuentes, J., Delgado, A., García‐López, A. M., & Cantero‐Martínez, C. (2025) · Soil and Tillage Research · doi:10.1016/j.still.2025.106496 · Cited by 7 · see citing works
Soil health responses to long-term grazing intensity gradients in two semiarid rangelands
Taylor, K., Derner, J., Liptzin, D., Porensky, L., Lavallee, J., Augustine, D., & Hoover, D. L. (2025) · Agriculture, Ecosystems & Environment · doi:10.1016/j.agee.2025.109548 · Cited by 7 · see citing works
Legume-based rotation enhance long-term soil carbon storage in eastern Oregon dryland wheat systems
Ramírez, P. B., Calderon, F. J., Singh, S., & Machado, S. (2025) · Scientific Reports · doi:10.1038/s41598-025-98916-x · Cited by 5
Self-citationLegacy of severe soil degradation hinders the buildup of mineral-associated soil organic carbon
Leal, O. D. A., Reichel, R., Wissel, H., & Brüggemann, N. (2025) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2025.179445 · Cited by 6 · see citing works
Binacchi, F., Niether, W., Brock, C., Knebl, L., Brændholt, A., Wolf, B., & Gattinger, A. (2023) · Agriculture, Ecosystems & Environment · doi:10.1016/j.agee.2023.108645 · Cited by 5
Prairie, A. M., Rosenzweig, S. T., Lundgren, J. G., Bredeson, M. M., Welch, K. D., Kane, D. A., & Cotrufo, M. F. (2025) · Soil and Tillage Research · doi:10.1016/j.still.2025.106896 · Cited by 5 · see citing works
Self-citationCrop rotation impacts on SOC fraction contents and stability: Insights from a global meta-analysis
Adingo, S., Jiang, R., Guo, Z., Jiang, F., Gao, L., Liu, J., Liu, S., & Peng, X. (2025) · Agriculture Ecosystems & Environment · doi:10.1016/j.agee.2025.110128 · Cited by 5 · see citing works
van der Pol, L. K., Crews, T. E., & Cotrufo, M. F. (2025) · Frontiers in Soil Science · doi:10.3389/fsoil.2025.1548577 · Cited by 4 · see citing works
Self-citationZhang, J.-L., Wu, B., Liu, M.-J., Jia, Y., Kang, L.-L., & Wang, G.-L. (2025) · Plant and Soil · doi:10.1007/s11104-025-07320-x · Cited by 4
“Consistent with previous studies (Mupangwa et al. 2021; van der Pol et al. 2022), we also found that alfalfa inclusion significantly increased the subsequent triticale yield, supporting our first hypothesis.”
Devaraju, N., Prudhomme, R., Lungarska, A., Wang, X., Yin, Z., de Noblet‐Ducoudré, N., Chakir, R., Jayet, P.-A., Brunelle, T., Viovy, N., et al. (2022) · Scientific Reports · doi:10.1038/s41598-022-24794-2 · Cited by 5 · see citing works
Lin, F., Liu, Y., Zhang, L., & Zhang, Y. (2026) · Agriculture · doi:10.3390/agriculture16010116 · Cited by 4 · see citing works
Assessing cropping system effects on carbon footprint on the Canadian prairies
Lin, S., Liu, K., & Lemke, R. (2025) · Soil Science Society of America Journal · doi:10.1002/saj2.70039 · Cited by 5 · see citing works
Wang, Y., Li, Y.-M., Qiao, Y.-B., Li, Y.-Y., You, J., Wu, P., & Wang, T. (2026) · Agricultural Water Management · doi:10.1016/j.agwat.2026.110304 · Cited by 2
Srivastava, R. K., Yetgin, A., & Omara, P. (2026) · Symbiosis · doi:10.1007/s13199-026-01130-0 · Cited by 2 · see citing works
“Under continuous monocropping, such low C: N inputs may stimulate active microbial turnover and accelerate carbon cycling, potentially limiting the accumulation of persistent mineral-associated organic carbon pools (Cong et al. 2015; van der Pol et al. 2022).”
Syromiatnykov, Y., Ishniyazova, S. A., Troyanovskaya, I., Voinash, S., Garkin, I., Malikov, V., & Orekhovskaya, A. (2026) · Advances in Agriculture · doi:10.1155/aia/5863850 · Cited by 2
Chemical properties of soil after over 55 years of differentiated fertilization and crop rotation
Suwara, I., Pawlak-Zaręba, K., Gozdowski, D., & Młynek, S. (2025) · Soil Science Annual · doi:10.37501/soilsa/213837 · Cited by 2
“The inclusion of legumes in crop rotation not only improves soil physical properties (e.g., reduces soil bulk density), but also increases organic matter and total nitrogen (Van Der Pol et al., 2022; Wang et al., 2023).”
Visscher, A. M., Fonte, S. J., Hartmann, M., Laub, M., Mucheru-Muna, M. W., Mugendi, D. N., Mutai, J. C., Ndungu, S. M., Six, J., Trivedi, P., et al. (2026) · Geoderma · doi:10.1016/j.geoderma.2026.117846 · Cited by 1 · see citing works
Zhang, M., Yu, R.-S., Cao, J., Li, W.-Q., Tang, S.-G., Chen, Z., Liu, H., Wang, X., Cai, J., & Zhang, H.-Y. (2026) · Frontiers in Plant Science · doi:10.3389/fpls.2026.1850670 · Cited by 1
The TaSDIR1‐4A‐TaASR4‐3D module regulates ABA‐related drought responses in wheat seedlings
Lei, Y.-H., Wang, Z., Li, L.-Q., Zhou, L., Wang, B., Chen, B., Qi, H.-D., Chen, J.-C., Cao, J., You, S., et al. (2026) · New Phytologist · doi:10.1111/nph.71322 · Cited by 1
“However, wheat is predominantly cultivated in arid and semiarid regions, rendering it highly susceptible to drought stress (Van der Pol et al ., 2022; Fu et al ., 2025).”
Labile Carbon dynamics under Initial Transition to Regenerative Agriculture
Roy, D., Kundu, R., Datta, A., Mandal, B., Paul, S., Sharma, S., & Ladha, J. (2026) · Soil Security · doi:10.1016/j.soisec.2026.100226 · Cited by 1
Weggler, K., Gerster, E., & Messner, J. (2025) · Frontiers in Sustainable Food Systems · doi:10.3389/fsufs.2025.1583852 · Cited by 1
Progressive Technologies in Agriculture Affecting the Quality of Primary Crop Production – a Review
Hašana, R., Jánoška, D., Bušo, R., Gavurníková, S., & Havrlentová, M. (2024) · Polnohospodarstvo · doi:10.2478/agri-2024-0012
Ramírez, P. B., Singh, S., Machado, S., Matamala, R., Singh, S., & Calderon, F. J. (2026) · Soil & Tillage Research · doi:10.1016/j.still.2025.107014 · Cited by 1
Self-citationBaiano, S., Rinaldi, M., Troccoli, A., Ciavarella, F., Sorrentino, M. C., & Morra, L. (2026) · Soil use and management · doi:10.1111/sum.70302
“In parallel, the introduction of legumes in crop rotations can increase the supply of high-quality C inputs, characterized by labile C, low C:N ratio and low molecular size (van der Pol et al. 2022).”
Григорова, А. А., Сушко, С. В., Пиголев, А. В., Непримерова, С. В., & Иващенко, К. В. (2026) · Агрофизика · doi:10.25695/agrph.2026.01.02
Babu, S., Singh, R., Mohapatra, K. P., Rathore, S., Kumar, S., Das, A., Idapuganti, R., Yadav, D., Kumar, A., Layek, J., et al. (2026) · Land Degradation & Development · doi:10.1002/ldr.70445
“Legume residue has a narrow C:N ratio and a greater percentage of low molecular weight compounds, which increase the C turnover in the soil system (Yadav, Babu, et al. 2021; van der Pol et al. 2022).”
Zhao, G., Li, S., JIANG, C., Ding, X., Feng, Z. H. O. U., Kaixian, W. U., Wu, B., & Fullen, M. A. (2026) · Pedosphere · doi:10.1016/j.pedsph.2026.01.015
Soil health response to soil biological conditioner in Brazilian soybean fields
da Luz, F. B., Cardoso, G. M., Rodrigues, A. M. D. S., Menillo, R. B., & Cherubin, M. (2025) · Experimental Agriculture · doi:10.1017/s0014479725000080
- Authors
- van der Pol, L. K., Robertson, A., Schipanski, M., Calderon, F. J., Wallenstein, M. D., & Cotrufo, M. F.
- Affiliations
- Shell (United States) (US)
- Colorado State University (US)
- Venue
- Agriculture, Ecosystems & Environment
- Publisher
- Elsevier BV
- Published
- 2022-06
- Volume
- 330
- Pages
- 107906
- ISSN
- 0167-8809 (ISSN-L)
- OpenAlex
- W4211118823
- Cited by
- 65
- References
- 113
- Citations by year
- 2026: 12 · 2025: 19 · 2024: 13 · 2023: 19 · 2022: 2
- FWCI
- 7.44
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Agriculture Ecosystems & Environment (publishedVersion, cc-by-nc-nd)
- Funders
- National Science Foundation
- National Institute of Food and Agriculture
- Topics
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
- Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Management and Crop Yield
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Agronomic Practices and Intercropping Systems
- Keywords
- Fabaceae, crop rotation, soil organic matter, wheat-fallow rotation, nitrogen stocks, particulate organic matter, mineral-associated organic matter, soil aggregate size, crop yield, no-tillage, Great Plains
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Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
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