Deep 16S rRNA metagenomics and quantitative PCR analyses of the premature infant fecal microbiota
Abstract
Metagenomic studies on the gut microbiota of preterm infants are scarce. We characterized the microbiota of 10 days-old neonates by deep 16S rRNA gene metagenomic analysis and compare the results with those obtained by qPCR. Both techniques lead to similar conclusions, allowing differentiating between preterm and full-term infants.
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Functional immaturity of the immune system and intestinal epithelium can influence the aberrant intestinal colonization pattern occurring in preterm neonates.
“Functional immaturity of the immune system and intestinal epithelium can influence the aberrant intestinal colonization pattern occurring in preterm neonates [18] .”
From Intestinal microbiota in health and disease: Role of bifidobacteria in gut homeostasis (2014) · cites this paper as [18]
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Self-citation
- Authors
- Arboleya, S., Ang, L., Margolles, A., Yiyuan, L., Dongya, Z., Liang, X., Solís, G., Fernández, N., de los Reyes-Gavilán, C. G., & Gueimonde, M.
- Affiliations
- Instituto de Productos Lácteos de Asturias (ES)
- BGI Group (China) (CN)
- Hospital Universitario De Cabueñes (ES)
- Consejo Superior de Investigaciones Científicas (ES)
- Corresponding author
- Gueimonde, Miguel
- Venue
- Anaerobe
- Publisher
- Elsevier BV
- Published
- 2012-06
- Volume
- 18
- Issue
- 3
- Pages
- 378-380
- ISSN
- 1075-9964 (ISSN-L)
- OpenAlex
- W2034216519
- Cited by
- 66
- References
- 16
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 4 · 2022: 4 · 2021: 5 · 2020: 2 · 2019: 5 · 2018: 6 · 2017: 7 · 2016: 11 · 2015: 5 · 2014: 10 · 2013: 3 · 2012: 1
- FWCI
- 2.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Anaerobe (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Keywords
- preterm infants, gut microbiota, fecal microbiota, 16S rRNA gene metagenomics, real-time PCR, neonatal gut colonization
- MeSH terms
- Bifidobacterium, Enterobacteriaceae, Feces, Female, Humans, Infant, Newborn, Infant, Premature, Lactobacillaceae, Male, RNA, Ribosomal, 16S, Streptococcaceae, Sequence Analysis, DNA, Infant, Very Low Birth Weight, Metagenome, Metagenomics, Molecular Typing, High-Throughput Nucleotide Sequencing, Real-Time Polymerase Chain Reaction
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