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Cited by 51Infant Nutrition and HealthGut microbiota and healthProbiotics and Fermented Foods

Assessment of intestinal microbiota modulation ability of Bifidobacterium strains in in vitro fecal batch cultures from preterm neonates

Arboleya, Silvia, Salazar, Nuria, Solís, Gonzalo, Fernández, Nuria, Hernández-Barranco, Ana M., Cuesta, Isabel, Gueimonde, Miguel, de los Reyes-Gavilán, Clara G.·2013·Anaerobe
DOI 10.1016/j.anaerobe.2012.11.001
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Abstract

Microbial colonization of the infant gut is essential for the development of the intestine and the immune system. The intestinal microbiota of full-term breast-fed infants is considered as the health standard for newborns. A culture medium containing formula milk was designed, which allowed a balanced growth of intestinal microorganisms and was used to perform fecal batch cultures from preterm babies. Sixteen Bifidobacterium strains and fructooligosaccharides (FOS) were tested for their ability to modulate in vitro the intestinal microbiota. The production of short chain fatty acids (SCFA) was measured by Gas Chromatography and the levels of some anaerobe (Bifidobacterium and Bacteroides groups) and facultative anaerobes (Enterobacteriaceae, Enterococcaceae, Weissella group, and Klebsiella pneumoniae) were determined by quantitative PCR. Results were referred to a fecal negative control culture without microorganisms or FOS added. Strains that in fecal cultures counteracted better the aberrancies previously found in feces of preterm babies, as compared with full-term breast-fed infants, were selected. The three Bifidobacterium bifidum strains tested in this work promoted the most suitable shifts in SCFA and in the ratio of variables facultative anaerobes to anaerobes. Two Bifidobacterium breve strains complied with the requirement for facultative anaerobes and anaerobes and one of them also promoted a suitable shift of SCFA. Bifidobacteria behaved similarly as FOS regarding the microbial profiles in fecal cultures but the production of lactic and acetic acid was much lower. B. breve and B. bifidum strains selected represent promising candidates for their assessment in more complex in vitro and in vivo models.

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  • In vitro and in vivo studies have shown that modulating bifidobacterial levels through probiotic or prebiotic supplementation can change the overall composition and metabolism of the gut microbiota.

    “In vitro and in vivo studies have shown that modulating bifidobacterial levels through probiotic or prebiotic supplementation can change the overall composition and metabolism of the gut microbiota (49–53).”

    From Bifidobacteria and Their Health-Promoting Effects (2017) · cites this paper as [49]

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Authors
Arboleya, S., Salazar, N., Solís, G., Fernández, N., Hernández-Barranco, A. M., Cuesta, I., Gueimonde, M., & de los Reyes-Gavilán, C. G.
Affiliations
  • Consejo Superior de Investigaciones Científicas (ES)
  • Hospital Universitario De Cabueñes (ES)
ORCID
Venue
Anaerobe
Publisher
Elsevier BV
Published
2013-02
Volume
19
Pages
9-16
ISSN
1075-9964 (ISSN-L)
DOI
10.1016/j.anaerobe.2012.11.001
OpenAlex
W2073931471
Cited by
51
References
44
Citations by year
2025: 1 · 2024: 3 · 2023: 4 · 2022: 3 · 2021: 7 · 2020: 2 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 10 · 2015: 6 · 2014: 3 · 2013: 2
FWCI
3.27
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Anaerobe (publishedVersion)
  • PubMed (publishedVersion)
  • DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
Topics
  • Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Keywords
Bifidobacterium, preterm infants, gut microbiota, Bifidobacterium bifidum, Bifidobacterium breve, fructooligosaccharides, short-chain fatty acids, anaerobic bacteria, facultative anaerobes, Enterobacteriaceae, Weissella, Bacteroides, probiotics, infant gut colonization
MeSH terms
Bifidobacterium, Chromatography, Gas, Culture Media, Fatty Acids, Feces, Humans, Infant, Newborn, Infant, Premature, Oligosaccharides, Biota, Real-Time Polymerase Chain Reaction

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