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Cited by 26Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyEscherichia coli research studies

Effects of purple prairie clover (Dalea purpurea Vent.) on feed intake, nutrient digestibility and faecal shedding of Escherichia coli O157: H7 in lambs

Huang, Q.Q., Jin, L., Xu, Z., Barbieri, L.R., Acharya, S., Hu, T.M., McAllister, T.A., Stanford, K. +1 more·2015·Animal Feed Science and Technology
DOI 10.1016/j.anifeedsci.2015.06.009
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Abstract

This study was conducted to assess the effects of purple prairie clover (PPC, Dalea purpurea Vent.) on feed intake, nutrient digestibility, faecal shedding of Escherichia coli O157:H7 and blood metabolites of lambs. A total of 18 lambs were divided into three groups and housed in individual pens and fed green chop (1) alfalfa (Alf), (2) a mixture of Alf and PPC in a ratio of 40:60 (DM basis; Mix) and (3) Mix supplemented with polyethylene glycol (Mix-P) for 45 d. A 333g/L polyethylene glycol (PEG) solution applied at the rate of 250mL/kg DM of Mix diet was included in the third treatment to neutralize the biological activity of condensed tannins (CT) in PPC. Total tract digestibility of the diets was determined using acid-insoluble ash (AIA) as an indigestible marker. Blood samples were collected to measure serum metabolites and to estimate the activity of antioxidant enzymes. All lambs were orally inoculated with a five strain mixture of E. coli O157:H7 after determination of diet digestibility and faecal samples were enumerated for E. coli O157:H7 after 1, 2, 3, 4, 7, 14, 21 and 28 d of inoculation. Feed intake did not differ among lambs fed the three diets. Organic matter digestibility (P<0.05) and protein digestibility (P<0.01) of Mix were lower than that of Alf. However, the three diets had similar neutral detergent fibre digestibility. Lambs fed Mix had lower levels (P<0.05) of serum creatinine and urea nitrogen than Alf, but both Mix and Mix-P had higher (P<0.05) levels of serum phosphorus. Mix fed lambs had greater (P<0.05) serum total antioxidant capacity but lower (P<0.05) serum superoxide dismutase activity than lambs fed Alf. After inoculation, lambs fed Mix shed less E. coli O157:H7 than lambs fed Alf between d 1 and d 7 (P<0.05–0.006), and lambs fed Mix-P between d 1 and d 3 (P<0.05–0.012). Lambs fed Alf shed more E. coli O157:H7 than lambs fed Mix (P<0.001) or Mix-P (P<0.01) over the 28-d post-challenge period. Incoportaion of PPC in lamb diets at 60% of DM reduced faecal shedding in lambs challenged with E. coli O157:H7 as compared to Alf. The fact that shedding of E. coli O157:H7 was also reduced with Mix-P suggests that the anti-E. coli O157:H7 response of PPC is not solely due to CT.

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Authors
Huang, Q. Q., Jin, L., Xu, Z., Barbieri, L. R., Acharya, S., Hu, T. M., McAllister, T. A., Stanford, K., & Wang, Y.
Affiliations
  • Agriculture and Agri-Food Canada (CA)
  • Northwest A&F University (CN)
Corresponding author
Wang, Y.
ORCID
Venue
Animal Feed Science and Technology
Publisher
Elsevier BV
Published
2015-09
Volume
207
Pages
51-61
ISSN
0377-8401 (ISSN-L)
DOI
10.1016/j.anifeedsci.2015.06.009
OpenAlex
W1948351530
Cited by
26
References
67
Citations by year
2025: 3 · 2024: 2 · 2022: 6 · 2021: 4 · 2020: 2 · 2018: 2 · 2017: 3 · 2016: 4
FWCI
6.51
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
Animal Feed Science and Technology (publishedVersion)
Funders
  • Ontario Ministry of Agriculture, Food and Rural Affairs
  • AAFC
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
Keywords
purple prairie clover, Dalea purpurea, Escherichia coli O157:H7, lambs, fecal shedding, proanthocyanidins, polyethylene glycol, feed intake, nutrient digestibility, blood metabolites, antioxidant enzymes, superoxide dismutase, serum total antioxidant capacity, serum phosphorus, blood urea nitrogen, serum creatinine

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