Cited by 149Ruminant Nutrition and Digestive PhysiologyAnimal Nutrition and PhysiologyMycotoxins in Agriculture and Food

Silage fermentation and bacterial community of bur clover, annual ryegrass and their mixtures prepared with microbial inoculant and chemical additive

Li, Ping, Zhang, Yu, Gou, Wenlong, Cheng, Qiming, Bai, Shiqie, Cai, Yimin·2019·Animal Feed Science and Technology

Abstract

The objective of this study was to evaluate the silage fermentation and bacterial community of bur clover, annual ryegrass and their mixtures in south of China. Bur clover, annual ryegrass and their mixtures were ensiled without additive (control), or with lactic acid bacteria inoculant (LABi, 10⁶ cfu/g of fresh weight) and formic acid (FA, 3 mL/kg of fresh weight) for 60 d of fermentation. Untreated bur clover displayed high bacterial diversity resulting in a poor fermentation. LABi and FA reduced bacterial diversity and enriched the relative abundance of Lactobacillus, Pediococcus or Sporolactobacillus species during ensiling, resulting in improved silage fermentation. Annual ryegrass contained relative abundance of Proteobacteria and Clostridium species, and produced more butyric acid. This study confirmed that LABi and FA could reduce microbial diversity and improve silage quality; and Clostridium species produced butyric acid which caused a poor fermentation.

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Crossref · 10.1016/j.anifeedsci.2018.11.009

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Authors
Li, P., Zhang, Y., Gou, W., Cheng, Q., Bai, S., & Cai, Y.
Affiliations
Japan International Research Center for Agricultural Sciences (JP)
Corresponding author
  • Bai, Shiqie
  • Cai, Yimin
Venue
Animal Feed Science and Technology
Publisher
Elsevier BV
Published
2019-01
Volume
247
Pages
285-293
ISSN
0377-8401 (ISSN-L)
OpenAlex
W2901379750
Cited by
149
References
33
Citations by year
2026: 10 · 2025: 14 · 2024: 18 · 2023: 29 · 2022: 32 · 2021: 26 · 2020: 17 · 2019: 3
FWCI
19.44
Type
journal article
Language
en
Open access
closed
Locations
Animal Feed Science and Technology (publishedVersion)
Funders
  • Sichuan Program for Key Research and Development (2017NZ0015, 18ZDYF1385)
  • earmarked fund for China Agriculture Research System (CARS35-35)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
Keywords
silage fermentation, Medicago polymorpha, Lolium multiflorum, forage mixtures, lactic acid bacteria inoculant, formic acid additive, bacterial communities, microbial diversity, Lactobacillus, Pediococcus, Sporolactobacillus, Clostridium, butyrate, silage quality

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