Silage fermentation and bacterial community of bur clover, annual ryegrass and their mixtures prepared with microbial inoculant and chemical additive
Abstract
The objective of this study was to evaluate the silage fermentation and bacterial community of bur clover, annual ryegrass and their mixtures in south of China. Bur clover, annual ryegrass and their mixtures were ensiled without additive (control), or with lactic acid bacteria inoculant (LABi, 10⁶ cfu/g of fresh weight) and formic acid (FA, 3 mL/kg of fresh weight) for 60 d of fermentation. Untreated bur clover displayed high bacterial diversity resulting in a poor fermentation. LABi and FA reduced bacterial diversity and enriched the relative abundance of Lactobacillus, Pediococcus or Sporolactobacillus species during ensiling, resulting in improved silage fermentation. Annual ryegrass contained relative abundance of Proteobacteria and Clostridium species, and produced more butyric acid. This study confirmed that LABi and FA could reduce microbial diversity and improve silage quality; and Clostridium species produced butyric acid which caused a poor fermentation.
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Bacteria play an important role in the whole silage process.
“bacteria play an important role in the whole silage process [43].”
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“Silage is composed of a community of microorganisms in a very complex environment [42], and bacteria play an important role in the whole silage process [43].”
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- Authors
- Li, P., Zhang, Y., Gou, W., Cheng, Q., Bai, S., & Cai, Y.
- Affiliations
- Japan International Research Center for Agricultural Sciences (JP)
- Corresponding author
- Bai, Shiqie
- Cai, Yimin
- Venue
- Animal Feed Science and Technology
- Publisher
- Elsevier BV
- Published
- 2019-01
- Volume
- 247
- Pages
- 285-293
- ISSN
- 0377-8401 (ISSN-L)
- OpenAlex
- W2901379750
- Cited by
- 149
- References
- 33
- Citations by year
- 2026: 10 · 2025: 14 · 2024: 18 · 2023: 29 · 2022: 32 · 2021: 26 · 2020: 17 · 2019: 3
- FWCI
- 19.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Animal Feed Science and Technology (publishedVersion)
- Funders
- Sichuan Program for Key Research and Development (2017NZ0015, 18ZDYF1385)
- earmarked fund for China Agriculture Research System (CARS35-35)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Agronomy and Crop Science › Ruminant Nutrition and Digestive Physiology
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Nutrition and Physiology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Keywords
- silage fermentation, Medicago polymorpha, Lolium multiflorum, forage mixtures, lactic acid bacteria inoculant, formic acid additive, bacterial communities, microbial diversity, Lactobacillus, Pediococcus, Sporolactobacillus, Clostridium, butyrate, silage quality
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